RNAi Phenotypes and the Localization of a Protein::GUS Fusion Imply a Role for Medicago truncatula PIN Genes in Nodulation

The symbiosis between legumes and rhizobia results in the development of a new plant organ, the nodule. A role for polar auxin transport in nodule development in Medicago truncatula has been demonstrated using molecular genetic tools. The expression of a DR5::GUS auxin-responsive promoter in uninoculated M. truncatula roots mirrored that reported in Arabidopsis, and expression of the construct in nodulating roots confirmed results reported in white clover. The localization of a root-specific PIN protein (MtPIN2) in normal roots, developing lateral roots and nodules provided the first evidence that a PIN protein is expressed in nodules. Reduced levels of MtPIN2, MtPIN3, and MtPIN4 mRNAs via RNA interference demonstrated that plants with reduced expression of various MtPINs display a reduced number of nodules. The reported results show that in M. truncatula, PIN proteins play an important role in nodule development, and that nodules and lateral roots share some early auxin responses in common, but they rapidly differentiate with respect to auxin and MtPIN2 protein distribution

The European Union and democratization in Central and Southeastern Europe since 1989

A central tool of the European Union (EU) to promote the democratization of post-communist Europe have been the conditions it has attached to the offer of accession. Yet EU’s influence varies across countries, and over time between the periods before and after accession. A key factor limiting the EU’s democratizing impact are domestic costs of complying with the EU’s conditionality: the more governments rely on illiberal and undemocratic means to maintain power, the less influence the EU has. Moreover, even if the domestic adjustment costs are not prohibitively high, for EU conditionality to bring about, or lock in democratic change, the positive and negative incentives relating to the benefits of EU membership have to be credible. The limited credibility of sanctions against backsliding in new members and of the reward of accession for current candidate countries in Southeastern Europe is a key explanation for the setback in the EU’s democratizing role during this decade

Metabolic Changes Following a 1-Year Diet and Exercise Intervention in Patients With Type 2 Diabetes

Objective—To characterize the relationships among longterm improvements in peripheral insulin sensitivity (glucose disposal rate [GDR]), fasting glucose, and free fatty acids (FFAs) and concomitant changes in weight and adipose tissue mass and distribution induced by lifestyle intervention in obese individuals with type 2 diabetes. Research Design And Methods—We measured GDR, fasting glucose, and FFAs during a euglycemic clamp and adipose tissue mass and distribution, organ fat, and adipocyte size by dual-energy X-ray absorptiometry, CT scan, and adipose tissue biopsy in 26 men and 32 women in the Look-AHEAD trial before and after 1 year of diet and exercise aimed at weight loss. Results—Weight and fasting glucose decreased significantly (P _ 0.0001) and significantly more in men than in women (_12 vs. _8% and _16 vs. _7%, respectively; P _ 0.05), while FFAs during hyperinsulinemia decreased and GDR increased significantly (P _ 0.00001) and similarly in both sexes (_53 vs. _41% and 63 vs. 43%; P _ NS). Men achieved a more favorable fat distribution by losing more from upper compared with lower and from deeper compared with superficial adipose tissue depots (P _ 0.01). Decreases in weight and adipose tissue mass predicted improvements in GDR but not in fasting glucose or fasting FFAs; however, decreases in FFAs during hyperinsulinemia significantly determined GDR improvements. Hepatic fat was the only regional fat measure whose change contributed independently to changes in metabolic variables. Conclusions—Patients with type 2 diabetes undergoing a 1-year lifestyle intervention had significant improvements in GDR, fasting glucose, FFAs and adipose tissue distribution. However, changes in overall weight (adipose tissue mass) and hepatic fat were the most important determinants of metabolic improvements.Jeanine B. Albu, Leonie K. Heilbronn, David E. Kelley, Steven R. Smith, Koichiro Azuma, Evan S. Berk, F. Xavier Pi-Sunyer, Eric Ravussin, and the Look AHEAD Adipose Research Grou

SolarFocus: A Solar Tracking and Reflecting System

Final report and team photo for Project 25 of ME450, Winter 2009 semester.This project aims to develop a simple, scalable, and low-cost motorized mirror system for the G.G. Brown building renovations at the University of Michigan. The mirrors will be directed by a light-sensing control system to track and reflect solar energy. The sunlight will be reflected into the North-facing windows of G.G. Brown – windows that do not normally receive direct sunlight. Each square meter of mirror can provide up to 1000 watts of energy, thereby displacing the equivalent fossil fuel usage and emissions. After piloting this technology in new G.G. Brown building, it can be generalized to residential and commercial applications.Peter Woolf (Chemical Engineering, U of M)http://deepblue.lib.umich.edu/bitstream/2027.42/62477/2/ME450 Winter2009 Team Photo - Project 25 - Solar Tracking and Reflecting System.jpghttp://deepblue.lib.umich.edu/bitstream/2027.42/62477/1/ME450 Winter2009 Final Report - Project 25 - Solar Tracking and Reflecting System.pd

Seeing and holding baby: Systematic review of clinical management and parental outcomes following stillbirth

Background - In 2009 there were an estimated 2.6 million stillbirths worldwide. In the United States, a 2007 systematic review found little consensus about professional behaviours perceived by parents to be most helpful or most distressing. In the United Kingdom a bereaved parents’ organisation has highlighted discordance between parental views and clinical guidelines that recommend clinicians’ do not encourage parents to see and hold their baby. The objective of this review was to identify and synthesise available research reporting parental outcomes relating to seeing and holding. Method(s) - We undertook a systematic review. We included studies of any design, reporting parental experiences and outcomes. Electronic searches (PubMed, PsychINFO) were conducted in January 2014. Three authors independently screened and assessed the quality of the studies, before abstracting data and undertaking thematic analysis. Results - We reviewed 741 records and included 23 studies (10 quantitative,12 qualitative,1 mixed-method). Twenty-one studies suggested positive outcomes for parents who saw or held their baby. Increased psychological morbidity was associated with current pregnancy, choice not to see their baby, lack of time with their baby and/or insufficient mementos. Three themes were formulated “Positive effects of contact within a traumatic life event”, “Importance of role of health professionals”; and “Impact on Mothers and Fathers: Similarities and differences”. Conclusions - Stillbirth is a risk factor for increased psychological morbidity. Parents’ seeing and holding their stillborn baby can be beneficial to their future wellbeing. Since 2007, there has been a proliferation of studies that challenge clinical guidelines recommending clinicians do not encourage parental contact

Shallow stratigraphic control on pockmark distribution in north temperate estuaries

This paper is not subject to U.S. copyright. The definitive version was published in Marine Geology 329-331 (2012): 34-45, doi:10.1016/j.margeo.2012.09.006.Pockmark fields occur throughout northern North American temperate estuaries despite the absence of extensive thermogenic hydrocarbon deposits typically associated with pockmarks. In such settings, the origins of the gas and triggering mechanism(s) responsible for pockmark formation are not obvious. Nor is it known why pockmarks proliferate in this region but do not occur south of the glacial terminus in eastern North America. This paper tests two hypotheses addressing these knowledge gaps: 1) the region's unique sea-level history provided a terrestrial deposit that sourced the gas responsible for pockmark formation; and 2) the region's physiography controls pockmarks distribution. This study integrates over 2500 km of high-resolution swath bathymetry, Chirp seismic reflection profiles and vibracore data acquired in three estuarine pockmark fields in the Gulf of Maine and Bay of Fundy. Vibracores sampled a hydric paleosol lacking the organic-rich upper horizons, indicating that an organic-rich terrestrial deposit was eroded prior to pockmark formation. This observation suggests that the gas, which is presumably responsible for the formation of the pockmarks, originated in Holocene estuarine sediments (loss on ignition 3.5–10%), not terrestrial deposits that were subsequently drowned and buried by mud. The 7470 pockmarks identified in this study are non-randomly clustered. Pockmark size and distribution relate to Holocene sediment thickness (r2 = 0.60), basin morphology and glacial deposits. The irregular underlying topography that dictates Holocene sediment thickness may ultimately play a more important role in temperate estuarine pockmark distribution than drowned terrestrial deposits. These results give insight into the conditions necessary for pockmark formation in nearshore coastal environments.Graduate support for Brothers came from a Maine Economic Improvement Fund Dissertation Fellowship

What can we learn from COVID-19?: examining the resilience of primary care teams

IntroductionThe COVID-19 pandemic continues to place an unprecedented strain on the US healthcare system, and primary care is no exception. Primary care services have shifted toward a team-based approach for delivering care in the last decade. COVID-19 placed extraordinary stress on primary care teams at the forefront of the pandemic response efforts. The current work applies the science of effective teams to examine the impact of COVID-19—a crisis or adverse event—on primary care team resilience.MethodsLittle empirical research has been done testing the theory of team resilience during an extremely adverse crisis event in an applied team setting. Therefore, we conducted an archival study by using large-scale national data from the Veterans Health Administration to understand the characteristics and performance of 7,023 Patient Aligned Care Teams (PACTs) during COVID-19.ResultsOur study found that primary care teams maintained performance in the presence of adversity, indicating possible team resilience. Further, team coordination positively predicted team performance (B = 0.53) regardless of the level of adversity a team was experiencing.DiscussionThese findings in turn attest to the need to preserve team coordination in the presence of adversity. Results carry implications for creating opportunities for teams to learn and adjust to an adverse event to maintain performance and optimize team-member well-being. Teamwork can act as a protective factor against high levels of workload, burnout, and turnover, and should be studied further for its role in promoting team resilience

The evolution of patterning during movement in a large-scale citizen science game.

The motion dazzle hypothesis posits that high contrast geometric patterns can cause difficulties in tracking a moving target and has been argued to explain the patterning of animals such as zebras. Research to date has only tested a small number of patterns, offering equivocal support for the hypothesis. Here, we take a genetic programming approach to allow patterns to evolve based on their fitness (time taken to capture) and thus find the optimal strategy for providing protection when moving. Our 'Dazzle Bug' citizen science game tested over 1.5 million targets in a touch screen game at a popular visitor attraction. Surprisingly, we found that targets lost pattern elements during evolution and became closely background matching. Modelling results suggested that targets with lower motion energy were harder to catch. Our results indicate that low contrast, featureless targets offer the greatest protection against capture when in motion, challenging the motion dazzle hypothesis

Detection Technology for IVHS, Volume II: Final Report Addendum

DTFH61-91-C-00076This addendum contains the results of analyses for an additional 39 runs. These runs were selected to be representative of the widest possible range of traffic and weather conditions that were encountered. The number of runs for each site analyzed in this addendum was chosen giving consideration to the amount of data collected at each particular site. The goal of the additional analysis was to study more results and thus, have a larger base by which to judge the performance of the technologies represented in the detector field tests. Understanding the operation of the detectors under test and the various layouts and configurations employed are an integral part of interpreting the results contained in this addendum. The necessary background information is contained in the Task L final report in Chapters 9 and 10 and is not reproduced in this addendum. Indeed, this volume is not intended to be a stand-alone document, but a complement to the work already published. The Detection Technology for IVHS project identified traffic parameters and their required accuracies for characterizing traffic flow in conventional and newer intelligent transportation systems (ITS), obtained state-of-the-art detectors, installed and evaluated them in three states having diverse climates, and studied the need and feasibility of establishing a national detector evaluation facility. The other report in this series is FHWA-RD-95-100, Volume I: Final Report

Intertemporal excess burden, bequest motives, and the budget deficit

The author aims to empirically determine the significant factors that affect the levels of budget deficits of central governments across time and across countries. He empirically tests two prominent theories of budget deficits-the Barro (1979) tax-smoothing approach, and the still-untested theory of negative bequest motives advocated by Cukierman and Meltzer (1989). The author uses econometric techniques including fixed-effects (both country and time) panel regressions spanning 87 countries over the period 1975 to 1992, and the Griliches treatment of missing data. The author finds relatively stronger statistical support for the tax-smoothing approach among developing countries but not in industrial countries. The existence of empirical evidence supporting the theory of negative bequest motives is indeterminate. The author also conducted post-regression analyses to assess the proportion of observed differences in budget deficits the factors were actually able to explain. These reveal that both theories are generally weak in accounting for inter-temporal changes in budget deficit shares for both industrial and developing countries. The theories performed significantly better in accounting for cross-section differences. The author has many contributions to the literature. First, he analyzes the question of what determines the size of central government budget deficits using cross-country time series data leading into the 1990s. Second, he provides empirical tests of the still-untested Cukierman-Meltzer (1989) negative bequest motive theory of budget deficits. By using the panel data, the author attempts to determine the factors that influence not only the inter-temporal differences in budget deficits but also those factors that lead to cross-country differences. Last but not least, he provides some preliminary evidence that poverty reduction is necessary for long-term government budget deficit reduction.Public Sector Economics&Finance,Environmental Economics&Policies,Economic Theory&Research,Banks&Banking Reform,Municipal Financial Management,Public Sector Economics&Finance,Economic Theory&Research,Economic Stabilization,Banks&Banking Reform,National Governance