Characterization of potato parents based on combining ability and heterosis for searching resistance to Phytophthora infestans

Controlar efectivamente a Phytophthora infestans, deviene de seleccionar genotipos con capacidad de transmitir resistencia. Con el objeto de caracterizar progenitores de papa en base a aptitud combinatoria y heterosis para resistencia a P. infestans, se cruzaron seis variedades (Libertas, Jaspe, Chotañawi, Pollerita, Robusta e INRA 92T.114.76). Quince familias de tubérculos de segunda generación clonal, obtenidas, fueron inoculadas con P. infestans en Balcarce (Buenos Aires, Argentina) y evaluadas bajo diseño en bloques completos aleatorizados con dos repeticiones. Se midió área bajo la curva del progreso de la enfermedad (AUDPC). Se estimó Aptitud Combinatoria (AC) general y específica, heterosis media, heterobeltiosis, heterosis específica (Hs) y heredabilidad en sentido amplio (H2) y estricto (h2). La AC fue significativa. El progenitor Robusta disminuyó la enfermedad estimada por AUDPC, el valor negativo grande de AC específica señaló mejor híbrido a Robusta x Chotañawi. Un tercio de las cruzas expresaron aditividad en todos los niveles de heterosis. Los valores de Hs manifestaron equivalencia con el porcentaje de disminución del AUDPC. Las heredabilidades (H2=0,63 y h2=0,54) indicaron que la selección por bajo AUDPC puede ser efectiva. El progenitor Robusta y la cruza Robusta x Chotañawi, conformaron los genotipos superiores recomendados para transferir resistencia a P. infestans.The efficient control of Phytopthora infestans results from the selection of genotypes with the capacity of transferring resistance. In order to characterize potato parents based on combining ability and heterosis for their resistance to P. infestans, six parents were crossed (Libertas, Jaspe, Chotañawi, Pollerita, Robusta and INRA 92T.114.76). The 15 tuber families of second clonal generation obtained were inoculated with P. infestans in Balcarce (Buenos Aires, Argentina) and evaluated under randomized complete block design with two replicates. Area Under Disease Progress Curve (AUDPC) was recorded for each genotype; general and specific combining ability, average heterosis, heterobeltiosis, specific heterosis and broad-sense (H2) and narrow (h2) heritability were estimated. Combining ability was significant. The Robusta parent reduced the disease estimated by AUDPC; Robusta x Chotañawi was the best hybrid, as observed by the high negative value of specific combining ability. One third of the crosses expressed significant additive effects for all levels of heterosis. Hs values showed equivalence with the percent reduction of AUDPC. The obtained heritabilities (H2=0.63 and h2=0.54) indicated that selection based on the low AUDPC values can be effective. Robusta parent and Robusta x Chotañawi cross are good genotypes for transmitting resistance to P. infestans.Fil: Andrade, Alberto Juan. Universidad Nacional de JujuyFil: Capezio, Silvia Beatriz.Fil: Huarte, Marcelo Atilio

Caracterización de progenitores de papa en base aaptitud combinatoria y heterosis para la búsqueda deresistencia a Phytophthora infestans = Characterization of potato parents based on combining ability and heterosis for searching resistance to Phytophthora infestans

Controlar efectivamente a Phytophthora infestans, deviene de seleccionar genotipos con capacidad de transmitir resistencia. Con el objeto de caracterizar progenitores de papa en base a aptitud combinatoria y heterosis para resistencia a P. infestans, se cruzaron seis variedades (Libertas, Jaspe, Chotañawi, Pollerita, Robusta e INRA92T.114.76). Quince familias de tubérculos de segunda generación clonal, obtenidas, fueron inoculadas con P. infestans en Balcarce (Buenos Aires, Argentina) y evaluadas bajo diseño en bloques completos aleatorizados con dos repeticiones. Se midió área bajo la curva del progreso de la enfermedad (AUDPC). Se estimó Aptitud Combinatoria (AC) general y específica, heterosis media, heterobeltiosis, heterosis específica (Hs) y heredabilidad en sentido amplio (H2) y estricto (h2). La AC fue significativa. El progenitor Robusta disminuyó la enfermedad estimada por AUDPC, el valor negativo grande de AC específica señaló mejor híbrido a Robusta x Chotañawi. Un tercio de las cruzas expresaron aditividad en todos los niveles de heterosis. Los valores de Hs manifestaron equivalencia con el porcentaje de disminución del AUDPC. Las heredabilidades (H2=0,63 y h2=0,54) indicaron que la selección por bajo AUDPC puede ser efectiva. El progenitor Robusta y la cruza Robusta x Chotañawi, conformaron los genotipos superiores recomendados para transferir resistencia a P. infestans.The efficient control of Phytopthora infestans results from the selection of genotypes with the capacity of transferring resistance. In order to characterize potato parents based on combining ability and heterosis for their resistance to P. infestans, six parents were crossed (Libertas, Jaspe, Chotañawi, Pollerita, Robusta and INRA 92T.114.76). The 15 tuber families of second clonal generation obtained were inoculated with P. infestans in Balcarce (Buenos Aires, Argentina) and evaluated under randomized complete block design with two replicates. Area Under Disease Progress Curve (AUDPC) was recorded for each genotype; general and specific combining ability, average heterosis, heterobeltiosis, specific heterosis and broad-sense (H2) and narrow (h2) heritability were estimated. Combining ability was significant. The Robusta parent reduced the disease estimated by AUDPC; Robusta x Chotañawi was the best hybrid, as observed by the high negative value of specific combining ability. One third of the crosses expressed significant additive effects for all levels of heterosis. Hs values showed equivalence with the percent reduction of AUDPC. The obtained heritabilities (H2=0.63 and h2=0.54) indicated that selection based on the low AUDPC values can be effective. Robusta parent and Robusta x Chotañawi cross are good genotypes for transmitting resistance to P. infestans.EEA BalcarceFil: Andrade, Alberto Juan. Universidad Nacional de Jujuy. Instituto de Biología de la Altura; ArgentinaFil: Capezio, Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentin

Caracterización de progenitores de papa en base a aptitud combinatoria y heterosis para la búsqueda de resistencia a Phytophthora infestans

The efficient control of Phytopthora infestans results from the selection of genotypes with the capacity of transferring resistance. In order to characterize potato parents based on combining ability and heterosis for their resistance to P. infestans, six parents were crossed (Libertas, Jaspe, Chotañawi, Pollerita, Robusta and INRA 92T.114.76). The 15 tuber families of second clonal generation obtained were inoculated with P. infestans in Balcarce (Buenos Aires, Argentina) and evaluated under randomized complete block design with two replicates. Area Under Disease Progress Curve (AUDPC) was recorded for each genotype; general and specific combining ability, average heterosis, heterobeltiosis, specific heterosis and broad-sense (H2) and narrow (h2) heritability were estimated. Combining ability was significant. The Robusta parent reduced the disease estimated by AUDPC; Robusta x Chotañawi was the best hybrid, as observed by the high negative value of specific combining ability. One third of the crosses expressed significant additive effects for all levels of heterosis. Hs values showed equivalence with the percent reduction of AUDPC. The obtained heritabilities (H2=0.63 and h2=0.54) indicated that selection based on the low AUDPC values can be effective. Robusta parent and Robusta x Chotañawi cross are good genotypes for transmitting resistance to P. infestans.Controlar efectivamente a Phytophthora infestans, deviene de seleccionar genotipos con capacidad de transmitir resistencia. Con el objeto de caracterizar progenitores de papa en base a aptitud combinatoria y heterosis para resistencia a P. infestans, se cruzaron seis variedades (Libertas, Jaspe, Chotañawi, Pollerita, Robusta e INRA92T.114.76). Quince familias de tubérculos de segunda generación clonal, obtenidas, fueron inoculadas con P. infestans en Balcarce (Buenos Aires, Argentina) y evaluadas bajo diseño en bloques completos aleatorizados con dos repeticiones. Se midió área bajo la curva del progreso de la enfermedad (AUDPC). Se estimó Aptitud Combinatoria (AC) general y específica, heterosis media, heterobeltiosis, heterosis específica (Hs) y heredabilidad en sentido amplio (H2) y estricto (h2). La AC fue significativa. El progenitor Robusta disminuyó la enfermedad estimada por AUDPC, el valor negativo grande de AC específica señaló mejor híbrido a Robusta x Chotañawi. Un tercio de las cruzas expresaron aditividad en todos los niveles de heterosis. Los valores de Hs manifestaron equivalencia con el porcentaje de disminución del AUDPC. Las heredabilidades (H2=0,63 y h2=0,54) indicaron que la selección por bajo AUDPC puede ser efectiva. El progenitor Robusta y la cruza Robusta x Chotañawi, conformaron los genotipos superiores recomendados para transferir resistencia a P. infestans

Discriminant analysis of principal components and pedigree assessment of genetic diversity and population structure in a tetraploid potato panel using SNPs

The reported narrow genetic base of cultivated potato (Solanum tuberosum) can be expanded by the introgression of many related species with large genetic diversity. The analysis of the genetic structure of a potato population is important to broaden the genetic base of breeding programs by the identification of different genetic pools. A panel composed by 231 diverse genotypes was characterized using single nucleotide polymorphism (SNP) markers of the Illumina Infinium Potato SNP Array V2 to identify population structure and assess genetic diversity using discriminant analysis of principal components (DAPC) and pedigree analysis. Results revealed the presence of five clusters within the populations differentiated principally by ploidy, taxonomy, origin and breeding program. The information obtained in this work could be readily used as a guide for parental introduction in new breeding programs that want to maximize variability by combination of contrasting variability sources such as those presented here.Fil: Deperi, Sofía Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Tagliotti, Martin Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Bedogni, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Manrique Carpintero, Norma C.. Michigan State University; Estados UnidosFil: Coombs, Joseph. Michigan State University; Estados UnidosFil: Zhang, Ruofang. Inner Mongolia University; ChinaFil: Douches, David. Michigan State University; Estados UnidosFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentin

Use of easy measurable phenotypic traits as a complementary approach to evaluate the population structure and diversity in a high heterozygous panel of tetraploid clones and cultivars

Diversity in crops is fundamental for plant breeding efforts. An accurate assessment of genetic diversity, using molecular markers, such as single nucleotide polymorphism (SNP), must be able to reveal the structure of the population under study. A characterization of population structure using easy measurable phenotypic traits could be a preliminary and low-cost approach to elucidate the genetic structure of a population. A potato population of 183 genotypes was evaluated using 4859 high-quality SNPs and 19 phenotypic traits commonly recorded in potato breeding programs. A Bayesian approach, Minimum Spanning Tree (MST) and diversity estimator, as well as multivariate analysis based on phenotypic traits, were adopted to assess the population structure. Results: Analysis based on molecular markers showed groups linked to the phylogenetic relationship among the germplasm as well as the link with the breeding program that provided the material. Diversity estimators consistently structured the population according to a priori group estimation. The phenotypic traits only discriminated main groups with contrasting characteristics, as different subspecies, ploidy level or membership in a breeding program, but were not able to discriminate within groups. A joint molecular and phenotypic characterization analysis discriminated groups based on phenotypic classification, taxonomic category, provenance source of genotypes and genetic background. Conclusions: This paper shows the significant level of diversity existing in a parental population of potato as well as the putative phylogenetic relationships among the genotypes. The use of easily measurable phenotypic traits among highly contrasting genotypes could be a reasonable approach to estimate population structure in the initial phases of a potato breeding program.Fil: Tagliotti, Martin Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Deperi, Sofía Irene. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Bedogni, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Zhang, Ruofang. Inner Mongolia University; ChileFil: Manrique Carpintero, Norma C.. Michigan State University; Estados UnidosFil: Coombs, Joseph. Michigan State University; Estados UnidosFil: Douches, David. Michigan State University; Estados UnidosFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentin

Situación de las virosis del cultivo de papa en el Sudeste Bonaerense

En el sudeste de la provincia de Buenos Aires se encuentra la principal región productora de papa de la Argentina. La papa que se produce tiene tres destinos: el mercado fresco, la industria y la producción de semilla, la cual se realiza en un área diferenciada. El cultivo de papa se establece a través de la propagación vegetativa y, como consecuencia, los tubérculos infectados se convierten en una vía de transmisión de virus. El objetivo fue determinar la incidencia de Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus X (PVX) y Tomato spotted wilt virus (TSWV) y la ocurrencia de infecciones mixtas entre estos virus en áreas de producción de papa para consumo e industria y papa-semilla del sudeste de Buenos Aires durante las campañas 2013-2014 a la 2018-2019. Otro objetivo fue analizar el progreso de los niveles de infección para estos virus y la tendencia de las categorías de certificación de papa-semilla fiscalizadas por el Instituto Nacional de semillas (INASE) en el área diferenciada en los últimos 35 años. En este estudio se trabajó con datos procedentes de tubérculos de papa de 107 lotes de consumo e industria recolectados según protocolo y 41 lotes de papa-semilla muestreados al azar por técnicos oficiales del INASE para su fiscalización. Los tubérculos fueron brotados y analizados mediante DAS-ELISA para detección de PVY, PLRV, PVX y TSWV. Se estimó la incidencia de cada virus, incidencia relativa entre estos y frecuencias de infecciones simples y mixtas para ambas categorías y para cada campaña. Se analizaron los niveles de infección de PVY, PLRV, PVX y TSWV del área diferenciada de producción de papa-semilla y su relación con las categorías de certificación. PVY resultó el virus con mayor incidencia mientras que PLRV y TSWV fueron los de menor incidencia tanto para la producción de papa destinada a consumo e industria como para papa-semilla. Se observó fluctuación interanual de los valores de incidencia para PVY, PLRV y TSWV en ambas categorías. Se reportó por primera vez infecciones mixtas entre PVY, PLRV y TSWV. Las infecciones mixtas más frecuentes fueron entre TSWV con PVY o PLRV. Se observó una disminución de los niveles de infección para PVY, PLRV, PVX y TSWV y una mejora en la tendencia de las categorías de certificación de papa-semilla para todos los virus en los últimos 35 años. Actualmente, las categorías de papa-semilla son Inicial iii para PVY e Inicial i para PLRV, PVX y TSWV. Desde 2010 no se detecta PVX en papa-semilla. Cabe señalar que la acción integrada de entidades públicas, privadas y productores que adoptaron el protocolo de la Mesa Provincial de papa logró que esta área diferenciada del sudeste de Buenos Aires se destaque por su excelencia sanitaria. No obstante, para la región de producción de papa para consumo e industria se deberían fortalecer las medidas de control de PVY y TSWV.The main potato producing area of Argentina is located in the southeast of Buenos Aires province. Potato is cropped for three purposes: fresh market, and industry and seed production; the latter is performed in a differentiated area. Potato crop is established through vegetative propagation; consequently, infected tubers become a route of virus transmission. The objective of this work was to determine the incidence of Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus X (PVX) and Tomato spotted wilt virus (TSWV), and the occurrence of mixed infections among these viruses in areas of potato production for fresh market, industry, and potato-seed of the southeast of Buenos Aires between the 2013-2014 and the 2018-2019 crop seasons. Another objective was to analyze the progress of the infection levels of these viruses and the trend of the potato-seed categories regulated by the “Instituto Nacional de Semillas” (INASE), produced in the differentiated area in the last 35 years. Data were collected from potato tubers corresponding to 107 plots destined for fresh market and industry, following sampling protocols, and from 41 potatoseed plots, which were randomly sampled by official INASE technicians for their control. These tubers were then sprouted and analyzed by DAS-ELISA for the detection of PVY, PLRV, PVX and TSWV. The incidence of each virus, relative incidence between them and frequencies of single and mixed infections were estimated for both categories and each season. Infection level of PVY, PLRV, PVX and TSWV of the differentiated potato-seed production area was analyzed. PVY was the virus with the highest incidence, whereas PLRV and TSWV had the lowest incidence in the production for fresh market, industry and potato-seed categories. Incidence values of PVY, PLRV and TSWV fluctuated among years in both categories. PVY, PLRV and TSWV mixed infection percentages were low, with double infections being more frequent than triple ones for both categories. TSWV co-infecting with PVY or PLRV were the most frequent mixed infections. This is the first report of mixed infections between PVY, PLRV and TSWV in potato in Argentina. The infection level for PVY, PLRV, PVX and TSWV decreased in the potato-seed differentiated area throughout the seasons. At the same time, there was an improvement in the trend of potato-seed certification categories regulated by INASE for all viruses in the last 35 years. Currently, the potato-seed category is at Initial III infection level for PVY and Initial I for PLRV, PVX and TSWV. PVX has not been detected since 2010 in potato seeds. It should be noted that the integrated action of public and private organizations and producers that adopted the protocol of the Provincial Potato Board made this differentiated area of the southeast of Buenos Aries stand out for its sanitary excellence. In the region where potato is produced for the fresh market and industry, PVY and TSWV control measures should be strengthened.EEA BalcarceFil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Salvalaggio, Andrea Eugenia. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce). Ecofisiología de Cultivos y Productos Agrícolas BalcarceFil: Bruno, Cecilia Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra Estadística y Biometría; ArgentinaFil: Bruno, C. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Lopez Lambertini, Paola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentin

Use of chemicals to maintain quality potato minimally processed products

La demanda por el agregado de valor a los productos de origen agropecuario ha generado interés sobre la preservación de productos mínimamente procesados (PMP). La vida útil de los PMP es disminuida por la reacción de pardeamiento enzimático, la cual causa una pérdida en la calidad del alimento. En el presente trabajo se estudió el efecto de la adición de compuestos químicos sobre el contenido de ácido ascórbico, fenoles totales, la actividad de la enzima PPO y el color de los PMP de papa (Solanum tuberosum L.). Los tratamientos fueron combinaciones de diferentes concentraciones de metabisulfito de sodio (MS), ácido ascórbico (AA) y ácido cítrico (AC). El ensayo se realizó en dos variedades de papa, Spunta y Newen INTA. Los tratamientos con 1,5% de AA y 1% AC mantuvieron la luminosidad y aumentaron la concentración de ácido ascórbico y el contenido de fenoles totales en el producto durante los primeros 10 días de almacenamiento. Es posible disminuir la concentración de MS si se lo combina con AA para mantener los parámetros de calidad en papas cortadas refrigeradas.Demand for value addition to the products of agricultural origin has generated interest in the preservation of minimally processed products (PMP). The shelf life of the PMP is decreased by the enzymatic browning reaction, which causes a loss in the quality of food. In this study the effect of chemical addition on the content of ascorbic acid, total phenolics, the PPO enzyme activity and colour of PMP potato (Solanum tuberosum L.) was studied. Treatments were combinations of different concentrations of sodium metabisulfite (MS), ascorbic acid (AA) and citric acid (CA). The assay was performed in two potato varieties, Newen INTA and Spunta. Treatments with 1.5% AA and 1% AC kept the brightness and increased the concentration of ascorbic acid and total phenolic content in the product during the first 10 days of storage. May decrease the concentration of MS when combined with AA for maintain the quality parameters in refrigerated potatoes cuts.Fil: Garcia Procaccini, Luz Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Monti, M. Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentin

Genome-wide association analysis of agronomical and physiological traits linked to drought tolerance in a diverse potatoes (Solanum tuberosum) panel

Potatoes is often considered a drought-sensitive crop and its sustainable production is threatened due to frequent drought episodes. Drought tolerance is a complex trait of increasing importance in potatoes therefore its yield is threatened. The differential response of several physiological and agronomical traits was evaluated in a diverse potatoes panel under contrasting water regimes to identify quantitative trait loci (QTL) associated with drought tolerance using genome-wide association analysis with 4859 high-quality SNP markers. Phenotypic data were collected from multiple environments and years. QTL with diverse linkage disequilibrium blocks for proline concentration, water consumption and yield were detected on chromosomes 1, 4 and 10. These QTL detected were associated with known gene functions. The current study provides insights into the putative genes that controlled the responses to the drought tolerance in potatoes at physiological and agronomical levels. The QTL described in this work might be included in future marker-assisted selection programmes linked with drought tolerance in potatoes.EEA BalcarceFil: Tagliotti, Martin Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Tagliotti, Martin Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Deperi, Sofia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Bedogni, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Bedogni, Maria Cecilia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina

Association mapping to detect Phytophthora infestans Mont. De Bary resistance in tetraploid potato

Phytophthora infestans (Mont.) de Baryis found in all areas of potato (Solanum tuberosum L.) production in Argentine. Association mapping uses the existing diversity in a a population to identify loci responsible of phenotypic variation of a character. The studied population was formed by 159 genotypes of the germplasm collection of the Instituto Nacional de Tecnología Agropecuaria (INTA) Estación Experimental Agropecuaria (EEA) Balcarce potato breeding program. These genotypes are part of a larger population that was previously analyzed using single-nucleotide polymorphism (SNP). Phenotypic assessment was carried out in two locations over 3 yr. Environmental conditions allowed the presence of the disease in all year × location combination. Phenotypic variability was found for relative area under disease progress curve (RAUDPC) and yield parameters as, Total weight and Seed weight. Association mapping was carried out on 159 genotypes. Eight significant associations were found using Bonferroni methodology, with values of −log(p) ranging between 4.61 and 5.42 located at chromosomes 3 (solcap_snp_c2_16679), 4 (solcap_snp_c1_6126 and solcap_snp_c2_38243, solcap_snp_c2_54077), 5 (solcap_snp_c2_42374), 6 (solcap_snp_c2_33228), 7 (solcap_snp_c1_7407), and 10 (solcap_snp_c2_63) and eleven significant associations using permutation tests with values of −log(p) between 4.5 and 6.42 located at chromosomes 1(solcap_snp_c1_5150), 2 (solcap_snp_c1_13923), 4 (solcap_snp_c1_4178, and solcap_snp_c2_50004), 5 (solcap_snp_c2_11829 and solcap_snp_c2_50532), 7 (solcap_snp_c2_35057 and solcap_snp_c1_10011), and 9 (solcap_snp_c2_1915). Some of the SNPs detected are adjacent to genes that encode enzymes that participate in metabolic pathways that are activated in response to interaction with pathogens. Those markers might be included in breeding programs related to improve late blight resistance in potato cultivars.Fil: Deperi, Sofía Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Tagliotti, Martin Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Bedogni, María Cecilia. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentin

Genome‐wide association analysis of agronomical and physiological traits linked to drought tolerance in a diverse potatoes ( Solanum tuberosum ) panel

Potatoes is often considered a drought-sensitive crop and its sustainable production is threatened due to frequent drought episodes. Drought tolerance is a complex trait of increasing importance in potatoes therefore its yield is threatened. The differential response of several physiological and agronomical traits was evaluated in a diverse potatoes panel under contrasting water regimes to identify quantitative trait loci (QTL) associated with drought tolerance using genome-wide association analysis with 4859 high-quality SNP markers. Phenotypic data were collected from multiple environments and years. QTL with diverse linkage disequilibrium blocks for proline concentration, water consumption and yield were detected on chromosomes 1, 4 and 10. These QTL detected were associated with known gene functions. The current study provides insights into the putative genes that controlled the responses to the drought tolerance in potatoes at physiological and agronomical levels. The QTL described in this work might be included in future marker-assisted selection programmes linked with drought tolerance in potatoes.Fil: Tagliotti, Martin Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Deperi, Sofía Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Bedogni, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Huarte, Marcelo Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentin