A Case Study of the Perceptions of Character Education in a Large Urban Community

This case study seeks to provide insight into the community perception of character education in a large urban area that does not currently implement an explicit program in moral values. The community is established through adolescents in middle school, parents of the adolescents, school counselors and community members. Informants are selected in an attempt to ensure a diverse cross section of the middle school communities. Perceptions of character education are established through the process of interviews and a card sort involving a total N of 72 stakeholders. Each respondent is asked to provide definitions, synonyms and/or examples of a given moral value. This phase of the study is conducted to allow for clarification and identification of individual perceptions and definitions associated with a given moral value. This is followed by an open-ended questionnaire which permits respondents to validate the list of morals while at the same time offer additional input. In the final phase of the interview, respondents separate the moral values to distinguish the important from the less important. From the important values, they rank order the top 5. The results of the interviews indicate that there is no significant difference in the perception of character education among the various educational stakeholders in this urban community. Eighty four percent of the respondents participating in the interview feel that public education does have a role in teaching moral values. Ninety one percent of the respondents feel that there are common moral values regardless of ethnicity and religion. Based on the identification and rankings of the moral values, a pattern emerges that indicates possible community collaboration in character education

Visual Contact Pressure Estimation for Grippers in the Wild

Sensing contact pressure applied by a gripper can benefit autonomous and teleoperated robotic manipulation, but adding tactile sensors to a gripper's surface can be difficult or impractical. If a gripper visibly deforms, contact pressure can be visually estimated using images from an external camera that observes the gripper. While researchers have demonstrated this capability in controlled laboratory settings, prior work has not addressed challenges associated with visual pressure estimation in the wild, where lighting, surfaces, and other factors vary widely. We present a model and associated methods that enable visual pressure estimation under widely varying conditions. Our model, Visual Pressure Estimation for Robots (ViPER), takes an image from an eye-in-hand camera as input and outputs an image representing the pressure applied by a soft gripper. Our key insight is that force/torque sensing can be used as a weak label to efficiently collect training data in settings where pressure measurements would be difficult to obtain. When trained on this weakly labeled data combined with fully labeled data that includes pressure measurements, ViPER outperforms prior methods, enables precision manipulation in cluttered settings, and provides accurate estimates for unseen conditions relevant to in-home use.Comment: Accepted for presentation at the 2023 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2023

ForceSight: Text-Guided Mobile Manipulation with Visual-Force Goals

We present ForceSight, a system for text-guided mobile manipulation that predicts visual-force goals using a deep neural network. Given a single RGBD image combined with a text prompt, ForceSight determines a target end-effector pose in the camera frame (kinematic goal) and the associated forces (force goal). Together, these two components form a visual-force goal. Prior work has demonstrated that deep models outputting human-interpretable kinematic goals can enable dexterous manipulation by real robots. Forces are critical to manipulation, yet have typically been relegated to lower-level execution in these systems. When deployed on a mobile manipulator equipped with an eye-in-hand RGBD camera, ForceSight performed tasks such as precision grasps, drawer opening, and object handovers with an 81% success rate in unseen environments with object instances that differed significantly from the training data. In a separate experiment, relying exclusively on visual servoing and ignoring force goals dropped the success rate from 90% to 45%, demonstrating that force goals can significantly enhance performance. The appendix, videos, code, and trained models are available at https://force-sight.github.io/

Natalizumab affects T-cell phenotype in multiple sclerosis: implications for JCV reactivation

The anti-CD49d monoclonal antibody natalizumab is currently an effective therapy against the relapsing-remitting form of multiple sclerosis (RRMS). Natalizumab therapeutic efficacy is limited by the reactivation of the John Cunningham polyomavirus (JCV) and development of progressive multifocal leukoencephalopathy (PML). To correlate natalizumab-induced phenotypic modifications of peripheral blood T-lymphocytes with JCV reactivation, JCV-specific antibodies (serum), JCV-DNA (blood and urine), CD49d expression and relative abundance of peripheral blood T-lymphocyte subsets were longitudinally assessed in 26 natalizumab-treated RRMS patients. Statistical analyses were performed using GraphPad Prism and R. Natalizumab treatment reduced CD49d expression on memory and effector subsets of peripheral blood T-lymphocytes. Moreover, accumulation of peripheral blood CD8+ memory and effector cells was observed after 12 and 24 months of treatment. CD4+ and CD8+ T-lymphocyte immune-activation was increased after 24 months of treatment. Higher percentages of CD8+ effectors were observed in subjects with detectable JCV-DNA. Natalizumab reduces CD49d expression on CD8+ T-lymphocyte memory and effector subsets, limiting their migration to the central nervous system and determining their accumulation in peripheral blood. Impairment of central nervous system immune surveillance and reactivation of latent JCV, can explain the increased risk of PML development in natalizumab-treated RRMS subjects

Pre-Existing T- and B-Cell Defects in One Progressive Multifocal Leukoencephalopathy Patient

Progressive multifocal leukoencephalopathy (PML) usually occurs in patients with severe immunosuppression, hematological malignancies, chronic inflammatory conditions or receiving organ transplant. Recently, PML has also been observed in patients treated with monoclonal antibodies. By taking advantage of the availability of samples from a multiple sclerosis (MS) patient treated with natalizumab, the antibody anti-α4 integrin, who developed PML and was monitored starting before therapy initiation, we investigated the fate of T and B lymphocytes in the onset of PML. Real-time PCR was used to measure new T- and B-cell production by means of T-cell receptor excision circle (TREC) and K-deleting recombination excision circle (KREC) analysis and to quantify transcripts for CD34, terminal-deoxynucleotidyltransferase, and V pre-B lymphocyte gene 1. T- and B-cell subsets and T-cell heterogeneity were measured by flow cytometry and spectratyping. The data were compared to those of untreated and natalizumab-treated MS patients and healthy donors. Before therapy, a patient who developed PML had a low TREC and KREC number; TRECs remained low, while KRECs and pre-B lymphocyte gene 1 transcripts peaked at 6 months of therapy and then decreased at PML diagnosis. Flow cytometry confirmed the deficient number of newly produced T lymphocytes, counterbalanced by an increase in TEMRA cells. The percentage of naive B cells increased by approximately 70% after 6 months of therapy, but B lymphocyte number remained low for the entire treatment period. T-cell heterogeneity and immunoglobulins were reduced

Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity

Progressive multifocal leukoencephalopathy (PML) is an often fatal demyelinating disease caused by lytic infection of oligodendrocytes with JC virus (JCV). The development of PML in non-immunosuppressed individuals is a growing concern with reports of mortality in patients treated with mAb therapies. JCV can persist in the kidneys, lymphoid tissue and bone marrow. JCV gene expression is restricted by non-coding viral regulatory region sequence variation and cellular transcription factors. Because JCV latency has been associated with cells undergoing haematopoietic development, transcription factors previously reported as lymphoid specific may regulate JCV gene expression. This study demonstrates that one such transcription factor, Spi-B, binds to sequences present in the JCV promoter/enhancer and may affect early virus gene expression in cells obtained from human brain tissue. We identified four potential Spi-B-binding sites present in the promoter/enhancer elements of JCV sequences from PML variants and the non-pathogenic archetype. Spi-B sites present in the promoter/enhancers of PML variants alone bound protein expressed in JCV susceptible brain and lymphoid-derived cell lines by electromobility shift assays. Expression of exogenous Spi-B in semi- and non-permissive cells increased early viral gene expression. Strikingly, mutation of the Spi-B core in a binding site unique to the Mad-4 variant was sufficient to abrogate viral activity in progenitor-derived astrocytes. These results suggest that Spi-B could regulate JCV gene expression in susceptible cells, and may play an important role in JCV activity in the immune and nervous systems

Lymphocyte Subsets Show Different Response Patterns to In Vivo Bound Natalizumab—A Flow Cytometric Study on Patients with Multiple Sclerosis

Natalizumab is an effective monoclonal antibody therapy for the treatment of relapsing- remitting multiple sclerosis (RRMS) and interferes with immune cell migration into the central nervous system by blocking the α4 subunit of very-late activation antigen-4 (VLA-4). Although well tolerated and very effective, some patients still suffer from relapses in spite of natalizumab therapy or from unwanted side effects like progressive multifocal leukoencephalopathy (PML). In search of a routine-qualified biomarker on the effectiveness of natalizumab therapy we applied flow cytometry and analyzed natalizumab binding to α4 and α4 integrin surface levels on T-cells, B-cells, natural killer (NK) cells, and NKT cells from 26 RRMS patients under up to 72 weeks of therapy. Four-weekly infusions of natalizumab resulted in a significant and sustained increase of lymphocyte-bound natalizumab (p<0.001) which was paralleled by a significant decrease in detectability of the α4 integrin subunit on all lymphocyte subsets (p<0.001). We observed pronounced natalizumab accumulations on T and B cells at single measurements in all patients who reported clinical disease activity (n = 4). The natalizumab binding capacity of in vitro saturated lymphocytes collected during therapy was strongly diminished compared to treatment-naive cells indicating a therapy-induced reduction of α4. Summing up, this pilot study shows that flow cytometry is a useful method to monitor natalizumab binding to lymphocytes from RRMS patients under therapy. Investigating natalizumab binding provides an opportunity to evaluate the molecular level of effectiveness of natalizumab therapy in individual patients. In combination with natalizumab saturation experiments, it possibly even provides a means of studying the feasability of patient-tailored infusion intervals. A routine-qualified biomarker on the basis of individual natalizumab saturation on lymphocyte subsets might be an effective tool to improve treatment safety

Is Increased Spanish-Language Proficiency Associated with Increased Connectedness to Spanish-Speaking Patients Among Interdisciplinary Healthcare Providers?

Introduction and Objective. Language proficiency is a critical component of effective healthcare delivery, particularly in multilingual settings. This study investigates the relationship between Spanish language fluency and the volunteer experience of health profession volunteers at the Apopka Farmworkers Clinic, which serves a predominantly Spanish-speaking migrant farmworker community. The objective of this research is to discern how Spanish fluency influences volunteer educational experiences and perceived impact and effectiveness in caring for Spanish-speaking patients. Methods. Health profession volunteers from the University of Central Florida College of Medicine, Nursing, Physical Therapy, Social Work, Counselor Education, and University of Florida College of Pharmacy, as well as community healthcare providers who volunteered at the Apopka Farmworkers Clinic from Fall 2022 to Winter 2024, were enrolled in this study. Participants completed an electronic survey that gauged their experiences through a series of statements rated on a 5-point Likert scale. Those that did not select an affiliation with a school and those that did not complete any of the statements using the Likert scale were excluded from the study. The data was analyzed with descriptive statistics to summarize the data and will include comparative statistical analysis to explore correlations between reported Spanish language fluency and volunteer responses. Results. Survey responses with exclusion criteria applied yielded a total of 147 study participants (73 College of Medicine students, 12 counseling, 21 nursing, 9 physical therapy, 1 social work, 10 pharmacy, and 21 providers). Survey response medians were calculated for each question across different participant groups. The results demonstrated that volunteers with greater self-reported Spanish language fluency felt an increased ability to connect to Spanish-speaking patients (3.46/5 for no proficiency versus 4.75/5 for native proficiency). Spanish language proficiency also affected volunteers’ motivation to approach native speaker patients on their own (3.25/5 for no proficiency versus 4.48/5 for native proficiency). Furthermore, regardless of Spanish fluency level, nursing, counseling, and pharmacy students reported an overall lower connection to patients they were treating with median connection levels of 3.62, 3.47, and 3.15 (out of 5) respectively compared to healthcare professionals, medical students, physical therapy, and social work students who reported median connection levels of 4.5, 4.2, 4.44, and 4.5 (out of 5) respectively. Conclusions-Implications. Our findings further underscore the need for language education and support in health professional training across multiple health professions. The study will inform future curricular developments aimed at preparing healthcare students to work effectively in diverse linguistic environments, ultimately enhancing the quality of care for Spanish-speaking populations