Effectiveness of planned teaching programme on practice of post natal exercises among mothers who have undergone lower segment cesarean section

Background: To assess the effectiveness of planned teaching programme on practice of post natal exercises among mothers who have undergone Lower segment cesarean section (L.S.C.S) in selected hospital at Bangalore.Methods: compare the practice scores of postnatal exercises among LSCS mothers between control group and experimental group.Results: Results shows that in post test the control group had inadequate practice score with a mean of 16.33%, standard deviation of 9.44 and mean percentage of 19.60 % where as in post test the experimental group there was a significant mean practice score gain of 39.84 % and standard deviation of 12.58 and mean percentage 79.67 % with a differences of 60.07 %.Conclusions: Significant difference was found between control and experimental group practice score of mothers in all areas of post natal exercises

Comparative impact of platelet rich plasma and transforming growth factor-β on chondrogenic differentiation of human adipose derived stem cells

Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β. Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays. Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects

Efficacy of low-fat milk and yogurt fortified with vitamin D<inf>3</inf> on systemic inflammation in adults with abdominal obesity

Background The prevalence of vitamin D deficiency is increasing globally and is associated with an increased risk of metabolic syndrome, autoimmune disease, and cardiovascular disease. Vit D deficiency is also associated with increased systemic inflammation. The current study aimed to determine the efficacy of low-fat milk and yogurt fortified with 1500 IU nano-encapsulated vitamin D, on systemic inflammation in abdominal obese participants. Method This multi-center study was conducted using a 2.5-month parallel total-blind randomized clinical trial design. Two hundred and eighty nine subjects were allocated to four groups: low-fat milk fortified by 1500 IU nano-encapsulated vitamin D3 (200 mL/day). Simple milk (200 mL/day), low-fat yogurt fortified by 1500 IU nano-encapsulated vitamin D3 (150 g/day), and simple yogurt (150 g/day). Results The results showed that serum levels of neutrophils, lymphocytes, platelets and red blood cell distribution width (RDW) were significantly lower before and after the intervention in fortified dairy groups. The results showed that serum levels of neutrophils, lymphocytes, platelets, and RDW before and after intervention in the fortified dairy groups were significantly lower (p < 0.05). The values of = neutrophil to lymphocyte ratio (NLR), platelets to lymphocyte ratio, and RDW to platelets ratio (RPR) reduced significantly in the fortification group (p < 0.05). Conclusion Fortification with nano-encapsulated vitamin D3 of dairy products may decrease inflammation in individuals with abdominal obesity

A Newton method for capturing Pareto optimal solutions of fuzzy multiobjective optimization problems

In this study, a Newton method is developed to obtain (weak) Pareto optimal solutions of an unconstrained multiobjective optimization problem (MOP) with fuzzy objective functions. For this purpose, the generalized Hukuhara differentiability of fuzzy vector functions and fuzzy max-order relation on the set of fuzzy vectors are employed. It is assumed that the objective functions of the fuzzy MOP are twice continuously generalized Hukuhara differentiable. Under this assumption, the relationship between weakly Pareto optimal solutions of a fuzzy MOP and critical points of the related crisp problem is discussed. Numerical examples are provided to demonstrate the efficiency of the proposed methodology. Finally, the convergence analysis of the method under investigation is discussed

Platelet-derived Microparticles Increase the Expression of hTERT Gene in Umbilical Cord Mesenchymal Stem Cells

Background: Mesenchymal stem cells have been widely considered in clinical researches because of their self-renewality and differentiation into various tissues. Nevertheless, their limited in vitro life span, which occurs only after several divisions, makes some changes in these cells, which affects all of their characteristics and remarkably reduces their application. In this study, the effect of platelet-derived microparticles, a rich source of growth factors, proteins, enzymes and microRNAs, was evaluated on the expression of hTERT gene as one of the main factors, involved in aging process and cell longevity. Materials and methods: Umbilical cord-mesenchymal stem cells were used for this study. The cells were confirmed by evaluating their morphology and surface markers using inverted microscope and flow cytometry, respectively. Platelet microparticles were prepared by centrifuging platelet bags with different speeds, and their concentration was determined by Bradford assay. When confluency of cultured MSCs was 30%, cells were treated with 50 &micro;g/mL of microparticles for 5 days, Then, RNA was extracted and cDNA was synthesized. The quantitative expression of hTERT gene was assessed using Real-Time PCR. Results: The fibroblast-like cells were isolated from umbilical cord tissue, and their mesenchymal markers were approved by flowcytometry. The results of Real-Time PCR showed that the expression of hTERT gene was increased more than 3 times compared with control group. Conclusion: It can be concluded that platelet-derived microparticles can potentially be recognized as a suitable, safe and effective method in increasing the hTERT gene expression and maybe life span of mesenchymal stem cells; however, further investigations is needed. &nbsp