The characteristic of the complete chloroplast genome of Lithocarpus konishii (Fagaceae), a rare and endemic species in South China

Lithocarpus konishii, a rare species endemic to islands in South China, was evaluated as a vulnerable species (VU) by the ‘China Species Red List.’ Here, we first presented the complete chloroplast genome sequence of L. konishii. The chloroplast genome was 161,059 bp in length with 36.76% GC content, containing a small single-copy region (SSC, 18,967 bp), a large single-copy region (LSC, 90,250 bp), and a pair of inverted repeats (IRs, 25,921 bp each). A total of 139 genes were predicted, including 87 protein-coding genes (CDS), 8 rRNAs, and 44 tRNAs. Based on the concatenated shared unique CDS sequence dataset, maximum-likelihood and Bayesian inference methods were used to build the phylogenetic trees of 18 species from the Fagaceae family. Results indicated that L. konishii is closely related to L. longnux and L. pachyphyllus var. fruticosus, and forms a monophyly of the subfamily Castaneoideae with Castanopsis and Castanea. This study provides a theoretical basis for the conservation genomics of this endangered plant

Research and Application of In Situ Sample-Processing Methods for Rapid Simultaneous Detection of Pyrethroid Pesticides in Vegetables

A novel rapid and cost-effective pre-processing method for the simultaneous determination of pyrethroid pesticides in vegetables has been developed and validated. The process of pesticide extraction was carried out by the QuEChERS (quick, easy, cheap, effective, rugged and safe) method combined with filtration by filter paper, and cleanup was carried out by the multi-plug-filtration-cleanup (m-PFC) method with no centrifuge program during the whole process. The pre-processing method is optimized for gas chromatography (GC). The process is convenient and time saving, requiring just a few seconds per sample. The recovery rate (70–120%), limit of detection (0.0001–0.007 mg/kg), precision (0.2–9.3%) and accuracy for each analyte were determined in 10 representative vegetables with good results. Finally, the feasibility of the developed method was further confirmed by the successful determination of pyrethroid-pesticide residues in pyrethroid-containing practical samples within the processing method coupled with thin-layer chromatography and a colloidal-gold test strip

6G Near-field Technologies White Paper

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