2,007 research outputs found
Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells
We have investigated the properties of nerve cell precursors in hydra by analyzing the differentiation and proliferation capacity of interstitial cells in the peduncle of Hydra oligactis, which is a region of active nerve cell differentiation. Our results indicate that about 50% of the interstitial cells in the peduncle can grow rapidly and also give rise to nematocyte precursors when transplanted into a gastric environment. If these cells were committed nerve cell precursors, one would not expect them to differentiate into nematocytes nor to proliferate apparently without limit. Therefore we conclude that cycling interstitial cells in peduncles are not intermediates in the nerve cell differentiation pathway but are stem cells. The remaining interstitial cells in the peduncle are in G1 and have the properties of committed nerve cell precursors (Holstein and David, 1986). Thus, the interstitial cell population in the peduncle contains both stem cells and noncycling nerve precursors. The presence of stem cells in this region makes it likely that these cells are the immediate targets of signals which give rise to nerve cells
Parity Violation and the Nucleon-Nucleon System
The status of the field of nuclear parity violation---both experimental and
theoretical---is reviewed, with special emphasis on those results which have
been obtained since the 1985 review article by Adelberger and Haxton.Comment: 52 page standard Latex file, contribution to the volume "Symmetries
in Nuclear Physics," ed. W.C. Haxton and E. Henle
The properties of nerve cell precursors in hydra
Two signals, the head activator and an injury stimulus, control differentiation of nerve cells from uncommitted stem cells in hydra [Th. Holstein, H. C. Schaller, and C. N. David, (1986) Dev. Biol. 115, 9–17]. The time of action of these signals in the precursor cell cycle was determined. Methanol extracts of hydra containing 10−13 M head activator cause nerve cell commitment in S phase of the precursor cell cycle. Committed precursors complete the cell cycle, divide, and arrest in G1. Injury relieves the G1 block and precursors differentiate nerve cells. Under these conditions the time from commitment to nerve differentiation is 12 hr, the time from the end of S phase to nerve differentiation is 9 hr, and the time from the G1 block to nerve differentiation is 4 hr. Committed precursors blocked in G1 are unstable, decaying with a half-life of 12 hr if not stimulated to differentiate by an injury stimulus
Tentacle morphogenesis in hydra
Stimulation of tentacle-specific cell differentiation by the neuropeptide head activator was investigated in Hydra magnipapillata. Tentacle-specific sensory nerve cells were identified by a monoclonal antibody, NV1. Treatment of hydra with 1pM head activator for 18h stimulated differentiation of NV1+ nerve cells and tentacle epithelial cells in tissue from the distal gastric region. Head tissue and tissue from the proximal gastric region did not respond to head activator treatment with increased NV1+ differentiation. Hence the distal gastric region appears to be the site of tentacle formation in hydra. Tentacle precursors in head tissue seem to be committed since they fail to respond to head activator or to changes in tissue size with altered amounts of tentacle formation. We suggest that NV1 precursors form a complex with tentacle epithelial cell precursors, which then moves distally through the head region into the tentacles. The signal for NV1+ differentiation appears to be formation of this complex
Extracting Electric Polarizabilities from Lattice QCD
Charged and neutral, pion and kaon electric polarizabilities are extracted
from lattice QCD using an ensemble of anisotropic gauge configurations with
dynamical clover fermions. We utilize classical background fields to access the
polarizabilities from two-point correlation functions. Uniform background
fields are achieved by quantizing the electric field strength with the proper
treatment of boundary flux. These external fields, however, are implemented
only in the valence quark sector. A novel method to extract charge particle
polarizabilities is successfully demonstrated for the first time.Comment: 17 pages, 6 figures, a few clarifications added, published versio
Mini-Collagens in Hydra Nematocytes
We have isolated and characterized four collagen-related c-DNA clones (N-COL 1, N-COL 2, N-COL 3, N-COL 4) that are highly expressed in developing nematocytes in hydra. All four c-DNAs as well as their corresponding transcripts are small in size (600-1,000 bp). The deduced amino acid sequences show that they contain a central region consisting of 14 to 16 Gly-X-Y triplets. This region is flanked amino-terminal by a stretch of 14-23 proline residues and carboxy-terminal by a stretch of 6-9 prolines. At the NH2- and COOH-termini are repeated patterns of cysteine residues that are highly conserved between the molecules. A model is proposed which consists of a central stable collagen triple helix of 12-14 nm length from which three 9-22 nm long polyproline II type helices emerge at both ends. Disulfide linkage between cysteine- rich segments in these helices could lead to the formation of oligomeric network structures. Electrophoretic characterization of nematocyst extracts allows resolution of small proline-rich polypeptides that correspond in size to the cloned sequences
Influence of electron-phonon interaction on superexchange
We investigate the influence of electron-phonon coupling on the superexchange
interaction of magnetic insulators. Both the Holstein-Hubbard model where the
phonons couple to the electron density, as well as an extended Su, Schrieffer,
Heeger model where the coupling arises from modulation of the overlap integral
are studied using exact diagonalization and perturbative methods. In all cases
for both the adiabatic (but non-zero frequency) and anti-adiabatic parameter
regions the electron-phonon coupling is found to enhance the superexchange.Comment: 14 pages+4 postscript figure
Two-mode entanglement in two-component Bose-Einstein condensates
We study the generation of two-mode entanglement in a two-component
Bose-Einstein condensate trapped in a double-well potential. By applying the
Holstein-Primakoff transformation, we show that the problem is exactly solvable
as long as the number of excitations due to atom-atom interactions remains low.
In particular, the condensate constitutes a symmetric Gaussian system, thereby
enabling its entanglement of formation to be measured directly by the
fluctuations in the quadratures of the two constituent components [Giedke {\it
et al.}, Phys. Rev. Lett. {\bf 91}, 107901 (2003)]. We discover that
significant two-mode squeezing occurs in the condensate if the interspecies
interaction is sufficiently strong, which leads to strong entanglement between
the two components.Comment: 22 pages, 4 figure
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