Inflammation and lung injury in an ovine model of fluid resuscitated endotoxemic shock

Background Sepsis is a multi-system syndrome that remains the leading cause of mortality and critical illness worldwide, with hemodynamic support being one of the cornerstones of the acute management of sepsis. We used an ovine model of endotoxemic shock to determine if 0.9% saline resuscitation contributes to lung inflammation and injury in acute respiratory distress syndrome, which is a common complication of sepsis, and investigated the potential role of matrix metalloproteinases in this process. Methods Endotoxemic shock was induced in sheep by administration of an escalating dose of lipopolysaccharide, after which they subsequently received either no fluid bolus resuscitation or a 0.9% saline bolus. Lung tissue, bronchoalveolar fluid (BAL) and plasma were analysed by real-time PCR, ELISA, flow cytometry and immunohistochemical staining to assess inflammatory cells, cytokines, hyaluronan and matrix metalloproteinases. Results Endotoxemia was associated with decreased serum albumin and total protein levels, with activated neutrophils, while the glycocalyx glycosaminoglycan hyaluronan was significantly increased in BAL. Quantitative real-time PCR studies showed higher expression of IL-6 and IL-8 with saline resuscitation but no difference in matrix metalloproteinase expression. BAL and tissue homogenate levels of IL-6, IL-8 and IL-1β were elevated. Conclusions This data shows that the inflammatory response is enhanced when a host with endotoxemia is resuscitated with saline, with a comparatively higher release of inflammatory cytokines and endothelial/glycocalyx damage, but no change in matrix metalloproteinase levels

Cultiver le capital psychologique pour apprendre et rebondir d'un échec entrepreneurial

Bien que l’échec entrepreneurial soit perçu négativement dans la société, plusieurs chercheurs estiment qu’il offre une réelle opportunité d’apprentissage. Cependant, il est parfois difficile d’apprendre d’un échec compte tenu des différents coûts financiers, psychologiques et sociaux qu’il occasionne. Au travers d’un modèle conceptuel, nous proposons un angle d’approche plus positif de l’échec entrepreneurial. Nous suggérons qu’un niveau de capital psychologique élevé joue un rôle modérateur dans la relation entre les conséquences négatives de l’échec et l’apprentissage de l’échec. Cet apprentissage et ce capital psychologique élevé aideraient l’entrepreneur ayant échoué à poursuivre sa carrière entrepreneuriale