Reproducing the general through the local: Lessons from poverty research

Central to research into the conduct of everyday life are issues of generalisation. This chapter focuses on three interrelated forms or manifestations of generalisation, which invoke issues around how macro-level structures and intergroup relations are reproduced through micro-level situations. First, theoretical generalisation constitutes our efforts to enlarge the significance of small-scale exemplars through research by relating local insights to the broader body of academic knowledge. Second, referential generalisation involves relating everyday artefacts produced by our research participants to the broader social context and intergroup relations at play. Third, empathetic generalisation involves promoting witnessing, recognition, and empathy towards people experiencing poverty by people who are not living in poverty. These three forms or elements of generalisation are central to the development of action strategies to address issues of poverty

Cortical AAV-CNTF gene therapy combined with intraspinal mesenchymal precursor cell transplantation promotes functional and morphological outcomes after spinal cord injury in adult rats

Ciliary neurotrophic factor (CNTF) promotes survival and enhances long-distance regeneration of injured axons in parts of the adult CNS. Here we tested whether CNTF gene therapy targeting corticospinal neurons (CSN) in motor-related regions of the cerebral cortex promotes plasticity and regrowth of axons projecting into the female adult F344 rat spinal cord after moderate thoracic (T10) contusion injury (SCI). Cortical neurons were transduced with a bicistronic adeno-associated viral vector (AAV1) expressing a secretory form of CNTF coupled to mCHERRY (AAV-CNTFmCherry) or with control AAV only (AAV-GFP) two weeks prior to SCI. In some animals, viable or nonviable F344 rat mesenchymal precursor cells (rMPCs) were injected into the lesion site two weeks after SCI to modulate the inhibitory environment. Treatment with AAV-CNTFmCherry, as well as with AAV-CNTFmCherry combined with rMPCs, yielded functional improvements over AAV-GFP alone, as assessed by open-field and Ladderwalk analyses. Cyst size was significantly reduced in the AAV-CNTFmCherry plus viable rMPC treatment group. Cortical injections of biotinylated dextran amine (BDA) revealed more BDA-stained axons rostral and alongside cysts in the AAV-CNTFmCherry versus AAV-GFP groups. After AAV-CNTFmCherry treatments, many sprouting mCherry-immunopositive axons were seen rostral to the SCI, and axons were also occasionally found caudal to the injury site. These data suggest that CNTF has the potential to enhance corticospinal repair by transducing parent CNS populations

MRI Protocols Comparison of In-Vivo Spinal Cord Imaging Of the Rats for Segmentation Purposes

We are developing a three-dimensional (3D) model of the rat spinal cord to act as a reliable tool for investigating mechanisms of spinal injury. Segmentation and meshing, by which the spinal geometry is extracted into a model, requires both considerably clear boundaries and high-contrast representation of the cord components: white matter, grey matter, meninges, CSF and IVD. Unfortunately, despite the advancement of MRI technology, obtaining high-resolution and high-contrast spinal cord of the rat is not an easy task; tedious protocols set up are required. This work comparedtwoprotocols that are commonly used for in-vivo MRI acquisitions; FLASH (T1 weighted) and TurboRare(T2 weighted). The software packages MicroDICOM and Mimics were used to assess the outcome of each protocol. As further comparison, we also examined ex-vivo datasets obtaining with MSME (T2 and proton density weighted) acquisition. The assessment was made based on the clarity of canny edgecontrast detection and the amount of unintended objects adjacent to the spinal cord components on the 3D model. We found that compared to TurboRare (T2 weighted), FLASH (T1 weighted) visually produced higher contrast between white and grey matter. This visual assessment was confirmed by the canny edge boundary detection on MicroDICOM. On Mimics, this difference was not detectable. Although the amount of unintended structures of the 3D modelof FLASH (T1 weighted) acquisitions less than that of the TurboRare (T2 weighted), it was more likely due to the signal uniformity rather than the effect of acquisition selection. Additionally, since this work only involved 3 datasets, whereby the statistical validation is not sufficient, we could not suggesta conclusive finding. However, this work is important as the basis further MRI protocol comparison studies

Trace.space: a psychogeographical community project with members of an arts and health organisation

In this paper we theorise a situationist psychogeographical community group work project, conducted with members of an arts and health organization. Using creative ways to improve the mental health and well-being of individuals, we draw on the concepts of trace and spaces both to map relationships between researchers and group members and project implications. This project was driven by three aims which were: to do community group-work in order to produce contributions both inside and outside of the University; to use a psychogeographical approach to playfully critique everyday life in consumer capitalist society and finally, to consider the extent to which wider personal and political changes could be enabled. To realise these aims, we put into practice a range of architectural, critical community psychological and psychogeographical methods including photo-elicitation, dice walking and site specific investigations. We also facilitated participatory workshops via the creation of artistic and reflective maps and writing poems and stories. Reflections from all the stakeholders, conclusions and implications of this work are considered in terms of individual, group and societal changes. We argue for more psychogeographically inspired work

Work education and educational developments around sustainable livelihoods for sustainable career development and well-being

Covid-19, Artificial Intelligence (AI), and Climate Change, have disrupted work education, rendering sustainability of careers and livelihoods a concern. This paper outlines a collaborative response to that challenge, offering opportunities for sustainable livelihoods in a work education cloud collaboration, Project SLiC (Sustainable Livelihoods Work education and educational developments around sustainable livelihoods for sustainable career development and well-being Collaboration). We have joined forces across nation states in the Global South/North to share cloud resources, focused on teaching a postgraduate course, Sustainable Livelihoods. Online modules are stored in a secure cloud site, from which local courses draw-down, autochthonously, whichever resources fit workforce development in context. We outline modules, and an evaluative process, in a proof-of-concept trial. Finally, we envisage how this initial collaboration may morph into a whole degree, including research supervision. We close with a call to career development professionals to share their unique expertise and experiences at the work education frontline, on how to develop this sustainable careers project, for thegreater good

Does It Matter Who Writes Medical News Stories?

David Henry and colleagues review Australian news stories over a five-year period to assess whether quality is associated with who wrote the story: a specialist health journalist or a non-specialist

Immunohistochemical, ultrastructural and functional analysis of axonal regeneration through peripheral nerve grafts containing Schwann cells expressing BDNF, CNTF or NT3

Objective  To establish reference values for activated coagulation time (ACT) in normal cats and dogs, by visual assessment of clot formation using the MAX-ACTTM tube. Subjects  We recruited 43 cats and 50 dogs for the study; 11 cats and 4 dogs were excluded from the statistical analysis because of abnormalities on clinical examination or laboratory testing including anaemia, prolonged prothrombin time (PT) or activated partial thromboplastin time (APTT), or insufficient plasma volume for comprehensive laboratory coagulation testing. Procedure  Blood samples were collected via direct venipuncture for MAX-ACT, packed cell volume/total solids, manual platelet estimation and PT/APTT measurement. Blood (0.5 mL) was mixed gently in the MAX-ACT tube at 37°C for 30 s, then assessed for clot formation every 5 to 10 s by tipping the tube gently on its side and monitoring for magnet movement. The endpoint was defined as the magnet lodging in the clot. The technique was tested with 10 dogs by collecting two blood samples from the same needle insertion and running a MAX-ACT on each simultaneously. Results  In normal cats the mean MAX-ACT was 66 s (range 55–85 s). In normal dogs the mean was 71 s (range 55–80 s). There was no statistical difference between the first and second samples collected from the same needle insertion. Conclusions and Clinical Relevance  In both cats and dogs, a MAX-ACT result >85 s should be considered abnormal and further coagulation testing should be performed. Additionally, failure to discard the first few drops of the sample does not appear to significantly affect results

An annotated checklist of bryophytes of Europe, Macaronesia and Cyprus

Introduction. Following on from work on the European bryophyte Red List, the taxonomically and nomenclaturally updated spreadsheets used for that project have been expanded into a new checklist for the bryophytes of Europe. Methods. A steering group of ten European bryologists was convened, and over the course of a year, the spreadsheets were compared with previous European checklists, and all changes noted. Recent literature was searched extensively. A taxonomic system was agreed, and the advice and expertise of many European bryologists sought. Key results. A new European checklist of bryophytes, comprising hornworts, liverworts and mosses, is presented. Fifteen new combinations are proposed. Conclusions. This checklist provides a snapshot of the current European bryophyte flora in 2019. It will already be out-of-date on publication, and further research, particularly molecular work, can be expected to result in many more changes over the next few years.Peer reviewe

Setting a national consensus for managing mild and blast traumatic brain injury: post-meeting consensus report

A meeting was held on Wednesday 15 January 2020 to examine the current evidence for non-routine imaging and for neuroendocrine screening in the management of military personnel with brain injury and overlapping symptom domains. The Summit aimed to specifically address the relative utility of magnetoencephalography (MEG), diffusion tensor imaging (DTI) and susceptibility weighted imaging (SWI) in the UK context. This Consensus Report discusses points of consensus, points for further discussion/points of equipoise and recommendations that arose during, and following, the meeting

Psychology and poverty reduction: a global special Issue

A “global special issue” on poverty brought together 9 international psychology journals during 2010 through 2013. The purpose was to highlight psychology’s contribution toward the Millennium Development Goals (MDGs). These goals are rooted in the “capabilities approach” and highlight the importance of fostering environments that support 3 core domains: health, basic education, and income. Here, we analyze what the global special issue contributed. As a whole the global special issue provided an account of “how” psychology engages with poverty and poverty reduction. First, the global special issue, more than other research on poverty, was focused on lower- and middle-income settings. Second, while the content of the articles could be coded into 3 specific domains (health/well-being, education/development, and society/work), the vast majority of the articles straddled more than 1 category. Third, the contents of the global special issue could be organized in terms of the type of contribution: that is, practicality, theory, description, and advocacy. We highlight the importance of addressing wider situational and sociopolitical structures that constrain capability and potential, without losing sight of the person. Psychology might (a) concentrate resources on finding out what actually works to enable poverty reduction; and (b) apply what we know to ensure that research on poverty reduction is more informative and compelling to community stakeholders, organizations, and policymakers. Such an “implementation science” could advance poverty reduction and human development. (PsycINFO Database Record (c) 2014 APA, all rights reserved