84 research outputs found
N-type calcium current, Cav2.2, is enhanced in small diameter sensory neurons isolated from Nf1+/− mice
Major aspects of neuronal function are regulated by Ca2+ including neurotransmitter release, excitability, developmental plasticity, and gene expression. We reported previously that sensory neurons isolated from a mouse model with a heterozygous mutation of the Nf1 gene (Nf1+/−) exhibited both greater excitability and evoked release of neuropeptides compared to wildtype mice. Furthermore, augmented voltage-dependent sodium currents but not potassium currents contribute to the enhanced excitability. To determine the mechanisms giving rise to the enhanced release of substance P and calcitonin gene-related peptide in the Nf1+/− sensory neurons, the potential differences in the total voltage-dependent calcium current (ICa) as well as the contributions of individual Ca2+ channel subtypes were assessed. Whole-cell patch-clamp recordings from small diameter capsaicin-sensitive sensory neurons demonstrated that the average peak ICa densities were not different between the two genotypes. However, by using selective blockers of channel subtypes, the current density of N-type (Cav2.2) ICa was significantly larger in Nf1+/− neurons compared to wildtype neurons. In contrast, there were no significant differences in L-, P/Q- and R-type currents between the two genotypes. Quantitative real-time PCR measurements made from the isolated but intact dorsal root ganglia indicated that N-type (Cav2.2) and P/Q-type (Cav2.1) Ca2+ channels exhibited the highest mRNA expression levels although there were no significant differences in the levels of mRNA expression between the genotypes. These results suggest that the augmented N-type (Cav2.2) ICa observed in the Nf1+/− sensory neurons does not result from genomic differences but may reflect post-translational or some other non-genomic modifications. Thus, our results demonstrate that sensory neurons from Nf1+/− mice, exhibit increased N-type ICa and likely account for the increased release of substance P and calcitonin gene-related peptide that occurs in Nf1+/− sensory neurons
Dynamics and Instabilities of Planar Tensile Cracks in Heterogeneous Media
The dynamics of tensile crack fronts restricted to advance in a plane are
studied. In an ideal linear elastic medium, a propagating mode along the crack
front with a velocity slightly less than the Rayleigh wave velocity, is found
to exist. But the dependence of the effective fracture toughness on
the crack velocity is shown to destabilize the crack front if
. Short wavelength radiation due to weak random
heterogeneities leads to this instability at low velocities. The implications
of these results for the crack dynamics are discussed.Comment: 12 page
Stable propagation of an ordered array of cracks during directional drying
We study the appearance and evolution of an array of parallel cracks in a
thin slab of material that is directionally dried, and show that the cracks
penetrate the material uniformly if the drying front is sufficiently sharp. We
also show that cracks have a tendency to become evenly spaced during the
penetration. The typical distance between cracks is mainly governed by the
typical distance of the pattern at the surface, and it is not modified during
the penetration. Our results agree with recent experimental work, and can be
extended to three dimensions to describe the properties of columnar polygonal
patterns observed in some geological formations.Comment: 8 pages, 4 figures, to appear in PR
Solid 4He and the Supersolid Phase: from Theoretical Speculation to the Discovery of a New State of Matter? A Review of the Past and Present Status of Research
The possibility of a supersolid state of matter, i.e., a crystalline solid
exhibiting superfluid properties, first appeared in theoretical studies about
forty years ago. After a long period of little interest due to the lack of
experimental evidence, it has attracted strong experimental and theoretical
attention in the last few years since Kim and Chan (Penn State, USA) reported
evidence for nonclassical rotational inertia effects, a typical signature of
superfluidity, in samples of solid 4He. Since this "first observation", other
experimental groups have observed such effects in the response to the rotation
of samples of crystalline helium, and it has become clear that the response of
the solid is extremely sensitive to growth conditions, annealing processes, and
3He impurities. A peak in the specific heat in the same range of temperatures
has been reported as well as anomalies in the elastic behaviour of solid 4He
with a strong resemblance to the phenomena revealed by torsional oscillator
experiments. Very recently, the observation of unusual mass transport in hcp
solid 4He has also been reported, suggesting superflow. From the theoretical
point of view, powerful simulation methods have been used to study solid 4He,
but the interpretation of the data is still rather difficult; dealing with the
question of supersolidity means that one has to face not only the problem of
the coexistence of quantum coherence phenomena and crystalline order, exploring
the realm of spontaneous symmetry breaking and quantum field theory, but also
the problem of the role of disorder, i.e., how defects, such as vacancies,
impurities, dislocations, and grain boundaries, participate in the phase
transition mechanism.Comment: Published on J. Phys. Soc. Jpn., Vol.77, No.11, p.11101
A Flight Research Overview of WSPR, a Pilot Project for Sonic Boom Community Response
In support of NASAs ongoing effort to bring supersonic commercial travel to the public, NASA Dryden Flight Research Center and NASA Langley Research Center, in cooperation with other industry organizations, conducted a flight research experiment to identify the methods, tools, and best practices for a large-scale quiet (or low) sonic boom community human response test. The name of the effort was Waveforms and Sonic boom Perception and Response. Such tests will go towards building a dataset that governing agencies like the Federal Aviation Administration and International Civil Aviation Organization will use to establish regulations for acceptable sound levels of overland sonic booms. Until WSPR, there had never been an effort that studied the response of people in their own homes and performing daily activities to non-traditional, low sonic booms.WSPR was a NASA collaborative effort with several industry partners, in response to a NASA Aeronautics Research Mission Directorate Research Opportunities in Aeronautics. The primary contractor was Wyle. Other partners included Gulfstream Aerospace Corporation, Pennsylvania State University, Tetra Tech, and Fidell Associates, Inc.A major objective of the effort included exposing a community with the sonic boom magnitudes and occurrences expected in high-air traffic regions with a network of supersonic commercial aircraft in place. Low-level sonic booms designed to simulate those produced by the next generation of commercial supersonic aircraft were generated over a small residential community. The sonic boom footprint was recorded with an autonomous wireless microphone array that spanned the entire community. Human response data was collected using multiple survey methods. The research focused on essential elements of community response testing including subject recruitment, survey methods, instrumentation systems, flight planning and operations, and data analysis methods.This paper focuses on NASAs role in the efforts logistics and operations including human response subject recruitment, the operational processes involved in implementing the surveys throughout the community, instrumentation systems, logistics, flight planning, and flight operations. Findings discussed in this paper include critical lessons learned in all of those areas. The paper also discusses flight operations results. Analysis of the accuracy and repeatability of planning and executing the unique aircraft maneuver used to generate low sonic booms concluded that the sonic booms had overpressures within 0.15 pounds-per-square-feet of the planned values for 76 of t he attempts. Similarly, 90 of the attempts to generate low sonic booms within the community were successful
Diffusion and viscosity in a supercooled polydisperse system
We have carried out extensive molecular dynamics simulations of a supercooled
polydisperse Lennard-Jones liquid with large variations in temperature at a
fixed pressure. The particles in the system are considered to be polydisperse
both in size and mass. The temperature dependence of the dynamical properties
such as the viscosity () and the self-diffusion coefficients () of
different size particles is studied. Both viscosity and diffusion coefficients
show super-Arrhenius temperature dependence and fit well to the well-known
Vogel-Fulcher-Tammann (VFT) equation. Within the temperature range
investigated, the value of the Angell's fragility parameter (D )
classifies the present system into a strongly fragile liquid. The critical
temperature for diffusion () increases with the size of the
particles. The critical temperature for viscosity () is larger than
that for the diffusion and a sizeable deviations appear for the smaller size
particles implying a decoupling of translational diffusion from viscosity in
deeply supercooled liquid. Indeed, the diffusion shows markedly non-Stokesian
behavior at low temperatures where a highly nonlinear dependence on size is
observed. An inspection of the trajectories of the particles shows that at low
temperatures the motions of both the smallest and largest size particles are
discontinuous (jump-type). However, the crossover from continuous Brownian to
large length hopping motion takes place at shorter time scales for the smaller
size particles.Comment: Revtex4, 7 pages, 8 figure
Thermal fracture as a framework for quasi-static crack propagation
We address analytically and numerically the problem of crack path prediction
in the model system of a crack propagating under thermal loading. We show that
one can explain the instability from a straight to a wavy crack propagation by
using only the principle of local symmetry and the Griffith criterion. We then
argue that the calculations of the stress intensity factors can be combined
with the standard crack propagation criteria to obtain the evolution equation
for the crack tip within any loading configuration. The theoretical results of
the thermal crack problem agree with the numerical simulations we performed
using a phase field model. Moreover, it turns out that the phase-field model
allows to clarify the nature of the transition between straight and oscillatory
cracks which is shown to be supercritical.Comment: 19 pages, 8 figure
Designing for interaction immediacy to enhance social skills of children with autism
Children with Autism Spectrum Disorder often require therapeutic interventions to support engagement in effective social interactions. In this paper, we present the results of a study conducted in three public schools that use an educational and behavioral intervention for the instruction of social skills in changing situational contexts. The results of this study led to the concept of interaction immediacy to help children maintain appropriate spatial boundaries, reply to conversation initiators, disengage appropriately at the end of an interaction, and identify potential communication partners. We describe design principles for Ubicomp technologies to support interaction immediacy and present an example design. The contribution of this work is twofold. First, we present an understanding of social skills in mobile and dynamic contexts. Second, we introduce the concept of interaction immediacy and show its effectiveness as a guiding principle for the design of Ubicomp applications
Conspecific Aggression by Beavers (Castor canadensis) in the Sangamon River Basin in Central Illinois: Correlates with Habitat, Age, Sex and Season
A systematic literature review of pediculosis due to head lice in the Pacific Island Countries and Territories: what country specific research on head lice is needed?
- …