Rapid, sensitive and accurate method for determination of Lafutidine hydrochloride in human plasma by RP-HPLC system

ABSTRACT Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using Phenomenex Gemini c18 (4.6 x 250 mm, 5 µ) reverse phase column for the determination of LAF in human plasma, Solid Phase Extraction (SPE) technique was used for the extraction of analyte, detection was carried out by Photo Diode Array detector at 216 nm. Chromatographic resolution of the LAF was achieved within 4.6 min by using mobile phase Methanol and 5 mM Di-Potassium Hydrogen Phosphate Buffer (pH 9.5) (80:20, v/v), flow rate was 1.0 mL/min. Calibration curve was linear with correlation coefficient of 0.9996 in the range of 50-1000 ng/mL, Limit of Detection (LOD) and Limit of Quantitation (LOQ) were 10 ng/mL and 30 ng/mL respectively, intra and inter-day deviations were lower than 3.92 % and 3.98 % respectively. The overall mean recovery of LAF was 94.57 %. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC method was successfully validated and may be applied to conduct bioavailability & bioequivalence studies of LAF

Diisopropyl 1-(4-meth­oxy­phen­yl)-2,6-dimethyl-4-(3-nitro­phen­yl)-1,4-dihydro­pyridine-3,5-dicarboxyl­ate

In the title compound, C28H32N2O7, the 1,4-dihydro­pyridine ring adopts a flattened boat conformation. The two benzene rings are approximately perpendicular to the dihydro­pyridine ring, forming dihedral angles of 84.29 (9) and 82.96 (9)° with the mean plane of the 1,4-dihydro­pyridine unit, whereas the ester groups are only slightly twisted relative to this plane, with dihedral angles of 10.6 (1) and 9.0 (1)°

(E)-3-(Furan-2-yl)-1-(4-meth­oxy­phen­yl)prop-2-en-1-one

In the title mol­ecule, C14H12O3, the prop-2-en-1-one unit forms dihedral angles of 12.96 (5) and 7.89 (7)° with the 4-meth­oxy­phenyl group and the furan ring, respectively. The furan and benzene rings form a dihedral angle of 8.56 (5)°. In the crystal, C—H⋯π and π–π inter­actions are observed between the benzene and heterocyclic rings [centroid–centroid distance = 3.760 (1) Å]

Synthesis and characterization of some thiazolidinone derivatives possessing benzimidazole nucleus

ABSTRACT Synthesis of new thiazolidinone derivatives possessing benzimidazole nucleus by condensation reaction of various substituted mercapto acids in presence of anhydrous ZnCl 2. In our present study we have also synthesized Schiff bases of benzimidazole derivatives. All synthesized compounds were characterized by IR, 1 H NMR and Mass spectroscopy