Characterization of Elastic and Plastic Behaviors in Steel Plate Based on Eddy Current Technique Using a Portable Impedance Analyzer

A portable impedance analyzer (PIA) was developed based on a TiePie-HS3 device to provide the comparable impedance measurement accuracy of the Agilent 4294a impedance analyzer in the frequency range of 0~250 kHz. Then the PIA was applied to monitor the tensile stress-induced variation of the eddy current sensor’s impedance in a medium-carbon steel sample. A model of equivalent magnetic field induced by the elastic stress and the number of pinning sites indicated that the inductance of the eddy current loop firstly increased with the increase in the tensile stress and then decreased at the yield point of the material. The experimental results testified that the variation of impedance amplitude, the variation of phase angle, and the shift of two featured frequencies demonstrated opposite variation trends before and after the yield point, as predicated by the model. A new parameter, which combined the impedance variation information of the selected two frequencies, was found to exhibit nearly monotonous dependency on the tensile stress in elastic and plastic stages. The new parameter together with the developed portable impedance analyzer provided the solution to identify the elastic and plastic behaviors in ferromagnetic materials in practical applications with an eddy current technique

β-Asarone Increases Chemosensitivity by Inhibiting Tumor Glycolysis in Gastric Cancer

β-asarone is the main active ingredient of the Chinese herb Rhizoma Acori Tatarinowii, which exhibits a wide range of biological activities. It was confirmed to be an efficient cytotoxic agent against gastroenteric cancer cells. However, the exact mechanism of β-asarone in gastric cancer (GC) remains to be elucidated. The present study showed the inhibitory effect of β-asarone on three types of different differentiation stage GC cell lines (MGC803, SGC7901, and MKN74) in a dose-dependent manner. Meanwhile, the synergistic sensitivity of β-asarone and cisplatin was confirmed by using the median-effect principle. Flow cytometry assay revealed that under both normoxia and CoCl2-induced hypoxia conditions, β-asarone can induce apoptosis of GC cells, which can block GC cells in the cell cycle G2/M phase, showing obvious subdiploid peak. Moreover, the activity of lactic dehydrogenase (LDH), an enzyme that plays an important role in the final step of tumor glycolysis, was significantly decreased in GC cells following treatment with β-asarone. Mechanistically, β-asarone can reduce pyruvate dehydrogenase kinase (PDK) 1, phospho(p)-PDK1, PDK4, hypoxia-inducible factor 1-α (HIF1α), c-myc, STAT5, and p-STAT5 expression, which revealed how β-asarone affects tumor glycolysis. In conclusion, the present study provided evidence in support of the hypothesis that the increase of chemotherapy sensitization by β-asarone is associated with the inhibition of tumor glycolysis

Chemical Profiling, Quantitation, and Bioactivities of Ginseng Residue

Ginseng residue is a by-product stemming from the commercial extraction of ginsenosides. To assess the disparities between ginseng residue and ginseng tablet, we employed the ultra-high-performance liquid chromatography–quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) technique for sample analysis. The analyses revealed the presence of 39 compounds in both ginseng residue and ginseng tablets. Subsequently, the contents of total ginsenosides and total ginseng polysaccharides in the ginseng residue and ginseng tablet were determined. The results indicate that while only a small fraction of ginsenosides remained in the ginseng residue, a significant amount of polysaccharides was retained. Furthermore, our evaluation encompassed the antioxidant activities of both ginseng residue and ginseng tablets. Notably, ginseng residue exhibited robust antioxidant effects, thereby showcasing its potential for recycling as a functional food raw material

Improvement of uridine production of <i>Bacillus subtilis</i> by atmospheric and room temperature plasma mutagenesis and high-throughput screening

<div><p>In the present study, a novel breeding strategy of atmospheric and room temperature plasma (ARTP) mutagenesis was used to improve the uridine production of engineered <i>Bacillus subtilis</i> TD12np. A high-throughput screening method was established using both resistant plates and 96-well microplates to select the ideal mutants with diverse phenotypes. Mutant F126 accumulated 5.7 and 30.3 g/L uridine after 30 h in shake-flask and 48 h in fed-batch fermentation, respectively, which represented a 4.4- and 8.7-fold increase over the parent strain. Sequence analysis of the pyrimidine nucleotide biosynthetic operon in the representative mutants showed that proline 1016 and glutamate 949 in the large subunit of <i>B</i>. <i>subtilis</i> carbamoyl phosphate synthetase were of importance for the allosteric regulation caused by uridine 5′-monophosphate. The proposed mutation method with efficient high-throughput screening assay was proved to be an appropriate strategy to obtain uridine-overproducing strain.</p></div

Pyrimidine nucleoside produced by parent and mutant strains in 30 h shake-flask fermentation.

<p>Pyrimidine nucleoside produced by parent and mutant strains in 30 h shake-flask fermentation.</p

Relative uridine titer of representative mutants after different rounds of ARTP mutagenesis.

<p>(a) Mutants isolated from 200 mg/L, 300 mg/L and 400 mg/L 2-thiouracil-resistance plate; (b) Mutants isolated from 100 mg/L 6-azauracil-resistance plate; (c) Mutants isolated from 3 g/L 6-azauracil-resistance plate; (d) Mutants isolated from 200 mg/L 5-fluorouracil-resistance plate.</p

Procedure of high-throughput screening of <i>B</i>. <i>subtilis</i> mutants generated by ARTP.

<p>2-TU represents 2-thiouracil; 6-AU represents 6-azauracil; 5-FU represents 5-fluorouracil.</p

Time course of uridine production by mutant A219, A260, A566, F126 and parent strain TD12np in 48 h fed-batch fermentation.

<p>(a) Uridine titer; (b) Biomass; (c) Glucose consumption. Each plot represents the average of three samples.</p