Perencanaan dan pembuatan programming console plc omron dengan level otorisasi yang berbasis mikrokontroler mcs-51

Programming console adalah salah satu alat yang digunakan untuk memrogram PLC OMRON. Namun kelemahan programming console milik Omron ini ialah sistem keamanan yang tidak terjamin yaitu dengan menggunakan password yang bersifat umum. Kelemahan yang lain adalah penggunaan kunci dalam pemilihan mode selector sehingga membuat pengaksesan program PLC menjadi tidak praktis. Oleh karena itu, dalam tugas akhir ini dibuatlah programming console yang dapat membuka dan mengubah program dalam PLC dengan memasukkan nomor identitas dan password pengakses sebagai sistem keamanannya yang berbasis mikrokontroler MCS-51. Alat tugas akhir ini dilengkapi 39 keypad yang terdiri dari instruction keys, operational keys, dan numerical keys yang digunakan untuk memberi input ke mikrokontroler; Mikrokontroler tipe AT89C55WD digunakan sebagai tempat penyimpanan program; LCD sebagai sarana tampilan data; serial EEPROM AT24C08A untuk menyimpan data pengakses dan data instruksi yang didapat dari PLC; dan MAX232 sebagai sarana transfer data. Sedangkan alat pendukungnya adalah PLC OMRON sebagai tempat menyimpan dan menjalankan program; serta Host Link Communication sebagai sarana komunikasi antara mikrokontroler dengan PLC. Alat tugas akhir ini memiliki 3 mode pilihan. Mode pertama ialah monitor, mode kedua ialah program dan mode ketiga ialah database; yang terbagi dalam 5 level pengaksesan. Berdasarkan pengujian, semua mode tersebut dapat berfungsi dengan baik dan rata-rata waktu yang dibutuhkan untuk melakukan transfer data antara mikroprosesor dengan PLC pada proses monitor area memori yaitu di bawah 20ms

Transcriptomic Insights into the Insect Immune Response to Nematode Infection

Insects in nature interact with a wide variety of microbial enemies including nematodes. These include entomopathogenic nematodes that contain mutualistic bacteria and together are able to infect a broad range of insects in order to complete their life cycle and multiply, filarial nematodes which are vectored by mosquitoes, and other parasitic nematodes. Entomopathogenic nematodes are commonly used in biological control practices and they form excellent research tools for understanding the genetic and functional bases of nematode pathogenicity and insect anti-nematode immunity. In addition, clarifying the mechanism of transmission of filarial nematodes by mosquitoes is critical for devising strategies to reduce disease transmission in humans. In all cases and in order to achieve these goals, it is vital to determine the number and type of insect host genes which are differentially regulated during infection and encode factors with anti-nematode properties. In this respect, the use of transcriptomic approaches has proven a key step for the identification of insect molecules with anti-nematode activity. Here, we review the progress in the field of transcriptomics that deals with the insect response to nematode infection. This information is important because it will expose conserved pathways of anti-nematode immunity in humans

Culturing and Genetically Manipulating Entomopathogenic Nematodes

Entomopathogenic nematodes in the genera Heterorhabditis and Steinernema are obligate parasites of insects that live in the soil. The main characteristic of their life cycle is the mutualistic association with the bacteria Photorhabdus and Xenorhabdus, respectively. The nematode parasites are able to locate and enter suitable insect hosts, subvert the insect immune response, and multiply efficiently to produce the next generation that will actively hunt new insect prey to infect. Due to the properties of their life cycle, entomopathogenic nematodes are popular biological control agents, which are used in combination with insecticides to control destructive agricultural insect pests. Simultaneously, these parasitic nematodes represent a research tool to analyze nematode pathogenicity and host anti-nematode responses. This research is aided by the recent development of genetic techniques and transcriptomic approaches for understanding the role of nematode secreted molecules during infection. Here, a detailed protocol on maintaining entomopathogenic nematodes and using a gene knockdown procedure is provided. These methodologies further promote the functional characterization of entomopathogenic nematode infection factors

Pre-exposure to non-pathogenic bacteria does not protect Drosophila against the entomopathogenic bacterium Photorhabdus.

Immune priming in insects involves an initial challenge with a non-pathogenic microbe or exposure to a low dose of pathogenic microorganisms, which provides a certain degree of protection against a subsequent pathogenic infection. The protective effect of insect immune priming has been linked to the activation of humoral or cellular features of the innate immune response during the preliminary challenge, and these effects might last long enough to promote the survival of the infected animal. The fruit fly Drosophila melanogaster is a superb model to dissect immune priming processes in insects due to the availability of molecular and genetic tools, and the comprehensive understanding of the innate immune response in this organism. Previous investigations have indicated that the D. melanogaster immune system can be primed efficiently. Here we have extended these studies by examining the result of immune priming against two potent entomopathogenic bacteria, Photorhabdus luminescens and P. asymbiotica. We have found that rearing D. melanogaster on diet containing a non-pathogenic strain of Escherichia coli alone or in combination with Micrococcus luteus upregulates the antibacterial peptide immune response in young adult flies, but it does not prolong fly life span. Also, subsequent intrathoracic injection with P. luminescens or P. asymbiotica triggers the Immune deficiency and Toll signaling pathways in flies previously exposed to a live or heat-killed mix of the non-pathogenic bacteria, but the immune activation fails to promote fly survival against the pathogens. These findings suggest that immune priming in D. melanogaster, and probably in other insects, is determined by the type of microbes involved as well as the mode of microbial exposure, and possibly requires a comprehensive and precise alteration of immune signaling and function to provide efficient protection against pathogenic infection

A large deletion at the cortex locus eliminates butterfly wing patterning

AbstractAs the genetic basis of natural and domesticated variation has been described in recent years, a number of hotspot genes have been repeatedly identified as the targets of selection, HeliconiusivorycortexH. melpomeneHeliconiusivorycortexcortexivory-H. melpomeneivorycortexivor

Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78

As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for the production of Nannochloropsis sp. CCAP211/78 spanning four months of operation from July to October. Illumina sequencing of 16S rRNA gene amplicons demonstrated that a wide variety of bacteria were present in all reactor types, with predominance of Bacteroidetes and Alphaproteobacteria. Bacterial communities were significantly different between all reactor types (except between the horizontal tubular reactor and the vertical tubular reactor) and also between runs in each reactor. Bacteria common to the majority of samples included one member of the Saprospiraceae family and one of the NS11-12_marine group (both Bacteroidetes). Hierarchical clustering analysis revealed two phases during the cultivation period separated by a major shift in bacterial community composition in the horizontal tubular reactor, the vertical tubular reactor and the raceway pond with a strong decrease of the Saprospiraceae and NS11-12_marine group that initially dominated the bacterial communities. Furthermore, we observed a less consistent pattern of bacterial taxa appearing in different reactors and runs, most of which belonging to the classes Deltaproteobacteria and Flavobacteriia. In addition, canonical correspondence analysis showed that the bacterial community composition was significantly correlated with the nitrate concentration. This study contributes to our understanding of bacterial diversity and composition in different types of outdoor reactors exposed to a range of dynamic biotic and abiotic factors.Key points• Reactor types had significantly different bacterial communities except HT and VT• The inoculum source and physiochemical factors together affect bacterial community• The bacterial family Saprospiraceae is positively correlated to microalgal growt