Additional file 6: Table S5. of Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Results of unpaired t-test for comparisons indicated in Fig. 5b. (PDF 45 kb

Additional file 5: Table S4. of Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Results of unpaired t-test for comparisons indicated in Fig. 5a. (PDF 44 kb

Additional file 3: Table S2. of Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Estimated protein amounts, rate constants and specific activities for the first set of NbXIP1;1α mutants. (PDF 88 kb

Additional file 2: Figure S1. of Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Expression of N-terminally His-tagged NbXIP1;1α mutants in P. pastoris. Western blots showing the expression levels of N-terminally His-tagged NbXIP1;1 mutants in P. pastoris X-33 clones. Blots were developed by enhanced chemiluminiscence in a Syngene PXi touch instrument. a. The first set of NbXIP1;1 α mutants. NbXIP1;1αst is an N-terminally truncated construct of NbXIP1;1α used as control for the western blot. b. The second set of NbXIP1;1α mutants. The control is 2 μg of purified NbXIP1;1αwt. (PDF 207 kb

Additional file 7: Figure S2. of Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable

Cartoon representations of the homology models of NbXIP1;1αwt and NbXIP1;1α mutants. The pores in the models are shown in mesh representation from blue (widest) to red (narrowest). The HOLE program [38] was used to estimate the radius of the pore in the models. Starting from the top left to the bottom right; NbXIP1;1αwt, NbXIP1;1αL79G/I102H/V242I (mutant 1), NbXIP1;1αL79G/I102H/T246I (mutant 2), NbXIP1;1αL79G (mutant 3), NbXIP1;1αI102H (mutant 4), NbXIP1;1αV242I (mutant 5), NbXIP1;1αL79G/I102H (mutant 6), NbXIP1;1αI102H/V242I (mutant 7) and NbXIP1;1αL79G/V242I (mutant 8), respectively. See also Fig. 7. (PDF 187 kb