Low frequency transcranial electrical stimulation does not entrain sleep rhythms measured by human intracranial recordings

Transcranial electrical stimulation has widespread clinical and research applications, yet its effect on ongoing neural activity in humans is not well established. Previous reports argue that transcranial alternating current stimulation (tACS) can entrain and enhance neural rhythms related to memory, but the evidence from non-invasive recordings has remained inconclusive. Here, we measure endogenous spindle and theta activity intracranially in humans during low-frequency tACS and find no stable entrainment of spindle power during non-REM sleep, nor of theta power during resting wakefulness. As positive controls, we find robust entrainment of spindle activity to endogenous slow-wave activity in 66% of electrodes as well as entrainment to rhythmic noise-burst acoustic stimulation in 14% of electrodes. We conclude that low-frequency tACS at common stimulation intensities neither acutely modulates spindle activity during sleep nor theta activity during waking rest, likely because of the attenuated electrical fields reaching the cortical surface

Circulating adrenomedullin estimates survival and reversibility of organ failure in sepsis: the prospective observational multinational Adrenomedullin and Outcome in Sepsis and Septic Shock-1 (AdrenOSS-1) study

Background: Adrenomedullin (ADM) regulates vascular tone and endothelial permeability during sepsis. Levels of circulating biologically active ADM (bio-ADM) show an inverse relationship with blood pressure and a direct relationship with vasopressor requirement. In the present prospective observational multinational Adrenomedullin and Outcome in Sepsis and Septic Shock 1 (, AdrenOSS-1) study, we assessed relationships between circulating bio-ADM during the initial intensive care unit (ICU) stay and short-term outcome in order to eventually design a biomarker-guided randomized controlled trial. Methods: AdrenOSS-1 was a prospective observational multinational study. The primary outcome was 28-day mortality. Secondary outcomes included organ failure as defined by Sequential Organ Failure Assessment (SOFA) score, organ support with focus on vasopressor/inotropic use, and need for renal replacement therapy. AdrenOSS-1 included 583 patients admitted to the ICU with sepsis or septic shock. Results: Circulating bio-ADM levels were measured upon admission and at day 2. Median bio-ADM concentration upon admission was 80.5 pg/ml [IQR 41.5-148.1 pg/ml]. Initial SOFA score was 7 [IQR 5-10], and 28-day mortality was 22%. We found marked associations between bio-ADM upon admission and 28-day mortality (unadjusted standardized HR 2.3 [CI 1.9-2.9]; adjusted HR 1.6 [CI 1.1-2.5]) and between bio-ADM levels and SOFA score (p < 0.0001). Need of vasopressor/inotrope, renal replacement therapy, and positive fluid balance were more prevalent in patients with a bio-ADM > 70 pg/ml upon admission than in those with bio-ADM ≤ 70 pg/ml. In patients with bio-ADM > 70 pg/ml upon admission, decrease in bio-ADM below 70 pg/ml at day 2 was associated with recovery of organ function at day 7 and better 28-day outcome (9.5% mortality). By contrast, persistently elevated bio-ADM at day 2 was associated with prolonged organ dysfunction and high 28-day mortality (38.1% mortality, HR 4.9, 95% CI 2.5-9.8). Conclusions: AdrenOSS-1 shows that early levels and rapid changes in bio-ADM estimate short-term outcome in sepsis and septic shock. These data are the backbone of the design of the biomarker-guided AdrenOSS-2 trial. Trial registration: ClinicalTrials.gov, NCT02393781. Registered on March 19, 2015

The Metabolic Consequences of Hepatic AMP-Kinase Phosphorylation in Rainbow Trout

AMP-activated protein kinase (AMPK), a phylogenetically conserved serine/threonine protein kinase, is proposed to function as a “fuel gauge” to monitor cellular energy status in response to nutritional environmental variations. However, in fish, few studies have addressed the metabolic consequences related to the activation of this kinase. This study demonstrates that the rainbow trout (Oncorhynchus mykiss) possesses paralogs of the three known AMPK subunits that co-diversified, that the AMPK protein is present in the liver and in isolated hepatocytes, and it does change in response to physiological (fasting-re-feeding cycle) and pharmacological (AICAR and metformin administration and incubations) manipulations. Moreover, the phosphorylation of AMPK results in the phosphorylation of acetyl-CoA carboxylase, a main downstream target of AMPK in mammals. Other findings include changes in hepatic glycogen levels and several molecular actors involved in hepatic glucose and lipid metabolism, including mRNA transcript levels for glucokinase, glucose-6-phosphatase and fatty acid synthase both in vivo and in vitro. The fact that most results presented in this study are consistent with the recognized role of AMPK as a master regulator of energy homeostasis in living organisms supports the idea that these functions are conserved in this piscine model

Les lésions pigmentées vulvaires (de la théorie à la pratique, réalisation d'un CD-ROM)

PARIS-BIUM (751062103) / SudocCentre Technique Livre Ens. Sup. (774682301) / SudocSudocFranceF

Nineteen seventy-three national demographic survey of Tanzania : a new approach to national demographic surveys in Africa

Meeting: Population Association of Africa Meeting, 10-14 May 1974, Ibadan, NGPage 10 missingReport of the methodology used in a demographic survey designed to determine the effect of living conditions on fertility and mortality levels in Tanzania - examines survey areas ; methods of data collecting and data analysis; format of questionnaire; recruitment of researchers involved in field activity

Evidence for a Volcanic Cooling Signal in a 335-Year Coral Record from New Caledonia

Although volcanic cooling events have been detected in tree ring records, their occurrence in marine records has received much less attention, Herein we report results from a 335-year oxygen isotope record (1657-1992) from a New Caredonia coral indicating that as many as 16 interannual-scale cooling events occur within 1 year of a volcanic eruption as determined by ice core records, There are also pentadal/decadal-scale cooling events beginning in 1675, 1813, and 1903 that immediately postdate volcanic eruptions. However, the interannual correspondences are complicated by the fact that some of the cooling events also coincide with El Ninos, which cause cooling in this part of the western South Pacific. If our conclusions are substantiated by further work, occurrence of distinct volcanic cooling signals may enable refinement of coral chronologies by use of the \u27\u27event stratigraphic\u27\u27 approach, with the most promising correlation horizons being associated with the following eruptions. 1808 (unknown); 1813-1821 (several eruptions), 1835 (Coseguina), 1883 (Krakatau), and possibly 1963 (Agung)

A Multicentury Stable Isotope Record from a New Caledonia Coral: Interannual and Decadal Sea Surface Temperature Variability in the Southwest Pacific Since 1657 AD

A 335 year stable isotope record from a New Caledonia coral (22°S, 166°E) helps fill a large gap in historical climate reconstructions. Although the long‐term coral δ18O‐based sea surface temperature (SST) trend is one of warming, there are notable decadal fluctuations, especially in the early 18th and early 19th centuries. Mean annual SSTs between 1658 and 1900 are estimated to be ∼0.3°C lower than the 20th century average, with interdecadal excursions of 0.5°–0.8°C. Time series analyses of the coral isotope record reveals significant concentrations of variance in the El Niño band; an inderdecadal spectral peak is present, but its robustness requires additional statistical evaluation. A secular but irregular decrease in coral δ13C values begins in the mid‐1800s and may reflect the anthropogenic perturbation of the carbon reservoir. These and other results indicate that the New Caledonia coral isotope record is a valuable source of information on southwest Pacific climate history