Sol-gel synthesis of lithium doped mesoporous bioactive glass nanoparticles and tricalcium silicate for restorative dentistry: Comparative investigation of physico-chemical structure, antibacterial susceptibility and biocompatibility

Introduction: The sol-gel method for production of mesoporous bioactive glass nanoparticles (MBGNs) has been adapted to synthesize tricalcium silicate (TCS) particles which, when formulated with other additives, form the gold standard for dentine-pulp complex regeneration. Comparison of TCS and MBGNs obtained by sol-gel method is critical considering the results of the first ever clinical trials of sol-gel BAG as pulpotomy materials in children. Moreover, although lithium (Li) based glass ceramics have been long used as dental prostheses materials, doping of Li ion into MBGNs for targeted dental applications is yet to be investigated. The fact that lithium chloride benefits pulp regeneration in vitro also makes this a worthwhile undertaking. Therefore, this study aimed to synthesize TCS and MBGNs doped with Li by sol-gel method, and perform comparative characterizations of the obtained particles.Methods: TCS particles and MBGNs containing 0%, 5%, 10% and 20% Li were synthesized and particle morphology and chemical structure determined. Powder concentrations of 15mg/10 mL were incubated in artificial saliva (AS), Hank’s balanced saline solution (HBSS) and simulated body fluid (SBF), at 37°C for 28 days and pH evolution and apatite formation, monitored. Bactericidal effects against S. aureus and E. coli, as well as possible cytotoxicity against MG63 cells were also evaluated through turbidity measurements.Results: MBGNs were confirmed to be mesoporous spheres ranging in size from 123 nm to 194 nm, while TCS formed irregular nano-structured agglomerates whose size was generally larger and variable. From ICP-OES data, extremely low Li ion incorporation into MBGNs was detected. All particles had an alkalinizing effect on all immersion media, but TCS elevated pH the most. SBF resulted in apatite formation for all particle types as early as 3 days, but TCS appears to be the only particle to form apatite in AS at a similar period. Although all particles had an effect on both bacteria, this was pronounced for undoped MBGNs. Whereas all particles are biocompatible, MBGNs showed better antimicrobial properties while TCS particles were associated with greater bioactivity.Conclusion: Synergizing these effects in dental biomaterials may be a worthwhile undertaking and realistic data on bioactive compounds targeting dental application may be obtained by varying the immersion media

Factors that influence the quality of final impressions for fixed dental prostheses in Nairobi, Kenya

Background: Good quality dental impressions free of air bubbles, voids, steps, drags, streaks and tears are a pre-requisite for the fabrication of well-fitting fixed dental prostheses (FDP). The quality of impressions is dependent on clinician and material factors. Aim: To evaluate factors that influence the quality of final impressions for FDP in Nairobi, Kenya.  Methods: In this cross-sectional study, 234 impressions received by five dental laboratories were analyzed. The study collected information on the type of tray, impression material, technique, type of prostheses, and clinically detectable errors, including voids, inadequate material at margins, tears, steps, drags, and streaks. Impression quality was the outcome assessed as good, fair, or poor by two investigators. The independent variables influencing impression quality included clinician specialty, experience, impression material, technique, and tray type. Results: Inter-rater agreement was 96.8% (p<0.001). Clinician experience ranged between 1-45yrs, median 13.5yrs and mean 8.39±11.96yrs. The majority were GPs, 80.8% while restorative dentists were 11.5% and other specialists, 7.7%. Most impressions were non-aqueous elastomers, 97.9% employing dual-viscosity technique, 75.6%. Impression trays included stock metal, 60.3%, stock plastic, 34.2%, and custom, 5.5%. Impression quality was good, 24.8%, fair, 37.2% or poor, 38.0%. Cumulatively, 74.5% impressions had bubbles/voids, 53.0% tears and 43.2% poor margins. Clarity of margins was associated with clinician specialty, (Fisher’s exact=9.372, p=0.047), and impression technique with impression quality, (Pearson’s ?2 = 6.385, p=0.041). Compared to restorative specialists, estimated odds of other specialists producing poor margins was 5.71, 95%CI 1.55,21.06, Wald ?2=5.24, p=0.009 while for GPs, the estimated odds was 2.19, 95%CI 0.88, 5.43, Wald ?2 = 2.86, p=0.09. Compared to dual viscosity, estimated odds of monophase giving a poor-quality impression was 1.52, 95%CI 0.83,2.78, Wald ?2 = 1.52, p=0.18. Conclusion: Most impressions were good or fair hence acceptable. Quality of impressions was influenced by clinician specialty and impression technique

Developing a bioactive glass coated dental floss: antibacterial and mechanical evaluations

Abstract In the present study, we investigated commercial dental floss coated with chitosan or chitosan + mesoporous bioactive glass nanoparticles (MBGNs) in order to determine the antimicrobial and mechanical properties of the newly fabricated flosses. Whereas these coatings showed notable ability to inhibit growth of both Gram (+) and Gram (−) bacteria after 24 h, the impact was negligible at 3 h. Furthermore, the tensile strength of the floss was improved by the addition of these layers, making it more durable and effective for cleaning between teeth. We therefore propose enhanced investigations of these composites since they demonstrate enormous potential in promoting oral health. Graphical Abstrac