1,207 research outputs found
Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle:E5 and the HPV18 life cycle
Diffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis.
In most organisms, the number and distribution of crossovers that occur during meiosis are tightly controlled. All chromosomes must receive at least one 'obligatory crossover' and crossovers are prevented from occurring near one another by 'crossover interference'. However, the mechanistic basis of this phenomenon of crossover interference has remained mostly mysterious. Using quantitative super-resolution cytogenetics and mathematical modelling, we investigate crossover positioning in the Arabidopsis thaliana wild-type, an over-expressor of the conserved E3 ligase HEI10, and a hei10 heterozygous line. We show that crossover positions can be explained by a predictive, diffusion-mediated coarsening model, in which large, approximately evenly-spaced HEI10 foci grow at the expense of smaller, closely-spaced clusters. We propose this coarsening process explains many aspects of Arabidopsis crossover positioning, including crossover interference. Consistent with this model, we also demonstrate that crossover positioning can be predictably modified in vivo simply by altering HEI10 dosage, with higher and lower dosage leading to weaker and stronger crossover interference, respectively. As HEI10 is a conserved member of the RING finger protein family that functions in the interference-sensitive pathway for crossover formation, we anticipate that similar mechanisms may regulate crossover positioning in diverse eukaryotes
The response of microphytobenthos to physical disturbance, herbicide, and titanium dioxide nanoparticles exposure
The research leading to these results received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 730984, ASSEMBLE Plus project. AJW was funded by the John Templeton Grant 60501, “Putting the Extended Evolutionary Synthesis to the Test”. DMP and AJB were supported by the NERC Blue-coast award (NE/N016009/1).The microphytobenthos that form transient biofilms are important primary producers in intertidal, depositional habitats, yet we have only a limited understanding of how they respond to the cumulative impacts of the growing range of anthropogenic stressors to which they are exposed. We know even less about how the temporal alignment of exposure – such as duration and exposure sequence – may affect the response. Estuarine biofilms were cultured in mesocosms and exposed to the herbicide glyphosate and titanium dioxide (TiO2) nanoparticles in different sequences (glyphosate-first or TiO2-first), as well as in the presence and absence of physical disturbance. We found that at environmentally realistic chemical concentrations, the order of exposure was less important than the total stressor scenario in terms of impacts on key functional attributes and diatom community structure. Physical disturbance did not have an impact on functional attributes, regardless of exposure sequence.Publisher PDFPeer reviewe
Accreting coral reefs in a highly urbanized environment
Globally, many coral reefs have fallen into negative carbonate budget states, where biological erosion exceeds carbonate production. The compounding effects of urbanization and climate change have caused reductions in coral cover and shifts in community composition that may limit the ability of reefs to maintain rates of vertical accretion in line with rising sea levels. Here we report on coral reef carbonate budget surveys across seven coral reefs in Singapore, which persist under chronic turbidity and in highly disturbed environmental conditions, with less than 20% light penetration to 2 m depth. Results show that mean net carbonate budgets across Singapore’s reefs were relatively low, at 0.63 ± 0.27 kg CaCO3 m−2 yr−1 (mean ± 1 SE) with a range from − 1.56 to 1.97, compared with the mean carbonate budgets across the Indo-Pacific of 1.4 ± 0.15 kg CaCO3 m−2 yr−1, and isolated Indian Ocean reefs pre-2016 bleaching (~ 3.7 kg CaCO3 m−2 yr−1). Of the seven reefs surveyed, only one reef had a net negative, or erosional budget, due to near total loss of coral cover (\u3c 5% remaining coral). Mean gross carbonate production on Singapore’s reefs was dominated by stress-tolerant and generalist species, with low-profile morphologies, and was ~ 3 kg m−2 yr−1 lower than on reefs with equivalent coral cover elsewhere in the Indo-Pacific. While overall these reefs are maintaining and adding carbonate structure, their mean vertical accretion potential is below both current rates of sea level rise (1993–2010), and future predictions under RCP 4.5 and RCP 8.5 scenarios. This is likely to result in an increase of 0.2–0.6 m of water above Singapore’s reefs in the next 80 yr, further narrowing the depth range over which these reefs can persist
Growth and carbonate production of crustose coralline algae on a degraded turbid reef system
Crustose coralline algae (CCA) and other encrusting calcifiers drive carbonate production on coral reefs. However, little is known about the rates of growth and calcification of these organisms within degraded turbid reef systems. Here we deployed settlement cards (N = 764) across seven reefs in Singapore for two years to examine spatio-temporal variation in encrusting community composition and CCA carbonate production. Our results showed that CCA was the dominant encrusting taxa (63.7% ± 18.3SD) across reefs. CCA carbonate production rates (0.009–0.052 g cm−2 yr−1) were less than half of those reported for most Indo-Pacific reefs, but similar to other turbid reef systems. Highest CCA carbonate production rates were observed furthest from Singapore\u27s main shipping port, due to a relative increase in CCA cover on the offshore reefs. Our results suggest that proximity to areas of high industrialisation and ship traffic may reduce the cover of encrusting calcifying organisms and CCA production rates which may have negative, long-term implications for the stabilisation of nearshore reefs in urbanised settings
Determining the thermal histories of Apollo 15 mare basalts using diffusion modelling in olivine
Mare basalts collected at the Apollo 15 landing site can be classified into two groups. Based on differing whole-rock major element chemistry, these groups are the quartz-normative basalt suite and the olivine-normative basalt suite. In this study we use modelling of Fe-Mg interdiffusion in zoned olivine crystals to investigate the magmatic environments in which the zonation was formed, be that within the lunar crust or during cooling within a surficial lava flow, helping to understand the thermal histories of the two basalt suites. Interdiffusion of Fe-Mg in olivine was modelled in 29 crystals in total, from six olivine-normative basalt thin sections and from three quartz-normative basalt thin sections. We used a dynamic diffusion model that includes terms for both crystal growth and intracrystalline diffusion during magma cooling. Calculated diffusion timescales range from 5 to 24 days for quartz-normative samples, and 6 to 91 days for olivine-normative samples. Similarities in diffusion timescales point to both suites experiencing similar thermal histories and eruptive processes. The diffusion timescales are short (between 5 and 91 days), and compositional zonation is dominated by crystal growth, which indicates that the diffusion most likely took place during cooling and solidification within lava flows at the lunar surface. We used a simple conductive cooling model to link our calculated diffusion timescales with possible lava flow thicknesses, and from this we estimate that Apollo 15 lava flows are a minimum of 3–6 m thick. This calculation is consistent with flow thickness estimates from photographs of lava flows exposed in the walls of Hadley Rille at the Apollo 15 landing site. Our study demonstrates that diffusion modelling is a valuable method of obtaining information about lunar magmatic environments recorded by individual crystals within mare basalt samples
The fog of work: the necessity for black and white, and grey rules to ensure safe workplace behaviour
The research outlined here is the result of a multi-disciplinary project which brought together engineers, ergonomists, ethnographers, sociologists and psychologists, entitled “Management of OSH in Networked Systems of Production or Service Delivery: Comparisons between Healthcare, Construction and Logistics”. The project aims to identify what types of OSH knowledge and evidence are in circulation and how they interact with each other in networked organisation. More specifically, investigating how workers interpret the multifaceted information they are exposed to and how this interpretation, in dynamic work contexts, influences their behaviour. The data obtained illuminate how top-down rules (explicit information) and socially constructed knowledge manifest and combine in different types of organisations
Time scales of magma transport and mixing at Kīlauea Volcano, Hawai'i
Modelling of volcanic processes is strongly limited by a poor knowledge of the timescales of storage, mixing and final ascent of magmas into the shallowest portions of volcanic 'plumbing' systems immediately prior to eruption. It is impossible to measure these timescales directly; however, micro-analytical techniques provide indirect estimates based on the extent of diffusion of species through melts and crystals. Here, diffusion in olivine phenocrysts from the 1959 Kīlauea Iki eruption is used to constrain the timing of mixing events in the crustal plumbing system on timescales of months to years before eruption. The timescales derived from zonation of Fe-Mg in olivines, combined with contemporaneous geophysical data suggests mixing occurred on 3 timescales: (1) up to 2 years prior to eruption in the deep storage system, (2), in a shallow reservoir, between incoming hot melts and cooler, resident melt for several weeks to months prior to eruption, and (3), in the conduit and summit reservoir, between the resident magma and cooled surface lava, draining back into the vent on timescales of hours to several days during pauses between episodes. Synchronous inflation of the shallow reservoir with deep earthquake swarms and mixing suggests a fitfully open transcrustal magmatic system prior to and during eruption.We acknowledge NERC studentship funds (I. Sides) and a United States Geological Survey Jack Kleinman grant, which allowed samples for this study to be collected.This is the final version of the article. It first appeared from the Geological Society of America via https://doi.org/10.1130/G37800.
Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle:E5 and the HPV18 life cycle
A combination of mutations in AKR1D1 and SKIV2L in a family with severe infantile liver disease.
Infantile cholestatic diseases can be caused by mutations in a number of genes involved in different hepatocyte molecular pathways. Whilst some of the essential pathways have a well understood function, such as bile biosynthesis and transport, the role of the others is not known. Here we report the findings of a clinical, biochemical and molecular study of a family with three patients affected with a severe infantile cholestatic disease. A novel homozygous frameshift germline mutation (c.587delG) in the AKR1D1 gene; which encodes the enzyme Δ 4-3-oxosteroid 5β-reductase that is required for synthesis of primary bile acids and is crucial for establishment of normal bile flow, was found in all 3 patients. Although the initial bile acid analysis was inconclusive, subsequent testing confirmed the diagnosis of a bile acid biogenesis disorder. An additional novel homozygous frameshift mutation (c.3391delC) was detected in SKIV2L in one of the patients. SKIV2L encodes a homologue of a yeast ski2 protein proposed to be involved in RNA processing and mutations in SKIV2L were recently described in patients with Tricohepatoenteric syndrome (THES). A combination of autozygosity mapping and whole-exome-sequencing allowed the identification of causal mutations in this family with a complex liver phenotype. Although the initial 2 affected cousins died in the first year of life, accurate diagnosis and management of the youngest patient led to successful treatment of the liver disease and disease-free survival.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are
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