6,338 research outputs found
Scattering amplitudes from finite-volume spectral functions
A novel proposal is outlined to determine scattering amplitudes from
finite-volume spectral functions. The method requires extracting smeared
spectral functions from finite-volume Euclidean correlation functions, with a
particular complex smearing kernel of width which implements the
standard -prescription. In the limit these smeared
spectral functions are therefore equivalent to Minkowskian correlators with a
specific time ordering to which a modified LSZ reduction formalism can be
applied. The approach is presented for general scattering amplitudes
(above arbitrary inelastic thresholds) for a single-species real scalar field,
although generalization to arbitrary spins and multiple coupled channels is
likely straightforward. Processes mediated by the single insertion of an
external current are also considered. Numerical determination of the
finite-volume smeared spectral function is discussed briefly and the interplay
between the finite volume, Euclidean signature, and time-ordered
-prescription is illustrated perturbatively in a toy example.Comment: 22 pages, 2 figures, CERN-TH-2019-035, CP3-Origins-2019-006 DNRF9
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Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System.
The variable lymphocyte receptors (VLRs) consist of leucine rich repeats (LRRs) and comprise the humoral antibodies produced by lampreys and hagfishes. The diversity of the molecules is generated by stepwise genomic rearrangements of LRR cassettes dispersed throughout the VLRB locus. Previously, target-specific monovalent VLRB antibodies were isolated from sea lamprey larvae after immunization with model antigens. Further, the cloned VLR cDNAs from activated lamprey leukocytes were transfected into human cell lines or yeast to select best binders. Here, we expand on the overall utility of the VLRB technology by introducing it into a filamentous phage display system. We first tested the efficacy of isolating phage into which known VLRB molecules were cloned after a series of dilutions. These experiments showed that targeted VLRB clones could easily be recovered even after extensive dilutions (1 to 109). We further utilized the system to isolate target-specific "lampribodies" from phage display libraries from immunized animals and observed an amplification of binders with relative high affinities by competitive binding. The lampribodies can be individually purified and ostensibly utilized for applications for which conventional monoclonal antibodies are employed
Cool Customers in the Stellar Graveyard IV: Spitzer Search for Mid-IR excesses Around Five DAs
Hydrogen atmosphere white dwarfs with metal lines, so-called DAZs, require
external accretion of material to explain the presence of weak metal line
absorption in their photospheres. The source of this material is currently
unknown, but could come from the interstellar medium, unseen companions, or
relic planetesimals from asteroid belt or Kuiper belt analogues. Accurate
mid-infrared photometry of these white dwarfs provide additional information to
solve the mystery of this accretion and to look for evidence of planetary
systems that have survived post main sequence evolution. We present {\em
Spitzer} IRAC photometry accurate to 3% for four DAZs and one DA with
circumstellar absorption lines in the UV. We search for excesses due to unseen
companions or circumstellar dust disks. We use {\em Hubble Space Telescope}
NICMOS imaging of these white dwarfs to gauge the level of background
contamination to our targets as well as rule out common proper motion
companions to WD 1620-391. All of our targets show no excesses due to
companions 20 M, ruling out all but very low mass companions to these
white dwarfs at all separations. No excesses due to circumstellar disks are
observed, and we place limits on what types of disks may still be present.Comment: 18 pages, 8 figures, Accepted to A
Virtual histology of transgenic mouse embryos for high-throughput phenotyping.
Journal ArticleA bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos. The datasets generated by this method are immediately amenable to state-of-the-art computational methods of organ patterning analysis. This technique to assess embryo anatomy represents a significant improvement in resolution, time, and expense for the quantitative, three-dimensional analysis of developmental patterning defects attributed to genetically engineered mutations and chemically induced embryotoxicity
Evaluation of the Importance of Time-Frequency Contributions to Speech Intelligibility in Noise
Recent studies on binary masking techniques make the assumption that each time-frequency (T-F) unit contributes an equal amount to the overall intelligibility of speech. The present study demonstrated that the importance of each T-F unit to speech intelligibility varies in accordance with speech content. Specifically, T-F units are categorized into two classes, speech-present T-F units and speech-absent T-F units. Results indicate that the importance of each speech-present T-F unit to speech intelligibility is highly related to the loudness of its target component, while the importance of each speech-absent T-F unit varies according to the loudness of its masker component. Two types of mask errors are also considered, which include miss and false alarm errors. Consistent with previous work, false alarm errors are shown to be more harmful to speech intelligibility than miss errors when the mixture signal-to-noise ratio (SNR) is below 0 dB. However, the relative importance between the two types of error is conditioned on the SNR level of the input speech signal. Based on these observations, a mask-based objective measure, the loudness weighted hit-false, is proposed for predicting speech intelligibility. The proposed objective measure shows significantly higher correlation with intelligibility compared to two existing mask-based objective measures
Multivariate Cepstral Feature Compensation on Band-limited Data for Robust Speech Recognition
Proceedings of the 16th Nordic Conference
of Computational Linguistics NODALIDA-2007.
Editors: Joakim Nivre, Heiki-Jaan Kaalep, Kadri Muischnek and Mare Koit.
University of Tartu, Tartu, 2007.
ISBN 978-9985-4-0513-0 (online)
ISBN 978-9985-4-0514-7 (CD-ROM)
pp. 144-151
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