A Comparison Pilot Study of Motor-level Electrical Stimulations for Lowering Postprandial Glucose Levels

Abstract Purpose: The purpose of this study is to compare the effects of three Motor-level Electrical Stimulation (MES) parameters, NMES, Russian current, and low-rate transcutaneous electric nerve stimulation (TENS) on non-diabetic healthy subjects’ postprandial glucose levels, heart rate, and oxygen consumption (VO2). Background: MES has been shown to improve glucose tolerance and glucose uptake in both animals and humans. The effects of MES include increasing the following: heart rate, blood pressure, oxygen uptake, ventilatory capacity, muscle bulk, muscle oxidative process, and muscle glycogen depletion. Recently, MES has been shown to improve blood glucose control in people with type 2 diabetes. However, limited research is available that comprehensively compares varying MES parameters on the effects of postprandial glucose levels. Methods: Twenty-eight healthy student subjects were randomly assigned to either the NMES, Russian current, TENS, or control group without any MES. Subjects participated in an overnight fast of at least 8 hours and had their fasting blood glucose measured. Subjects were given a glucose supplement to drink within 10 minutes, rested in supine for 30 minutes then the second glucose level was taken. Subjects received a 30 minute treatment intervention followed by the third blood glucose measurement. Subjects then rested an additional 30 minutes followed by obtaining the final blood glucose measurement. VO2 levels were monitored every 30 seconds, and heart rate was monitored every 3 minutes throughout the 90 minute study. Results: There were no significant differences between groups on glucose levels and heart rates throughout the study. The Russian group demonstrated a statistically significant increase up to 10% in VO2 compared to the control group. Conclusion: MES seems to have no effects on postprandial glucose levels in non-diabetic healthy subjects. While 30-minute MES treatment did not change the heart rate, Russian current significantly increased the VO2. Our findings indicate Russian current may have the potential to be applied to mimic exercise better than NMES or TENS. Further research is required to explore the effects of Russian current on blood glucose levels in people with T2D

A comparative study of S/MAR prediction tools

BACKGROUND: S/MARs are regions of the DNA that are attached to the nuclear matrix. These regions are known to affect substantially the expression of genes. The computer prediction of S/MARs is a highly significant task which could contribute to our understanding of chromatin organisation in eukaryotic cells, the number and distribution of boundary elements, and the understanding of gene regulation in eukaryotic cells. However, while a number of S/MAR predictors have been proposed, their accuracy has so far not come under scrutiny. RESULTS: We have selected S/MARs with sufficient experimental evidence and used these to evaluate existing methods of S/MAR prediction. Our main results are: 1.) all existing methods have little predictive power, 2.) a simple rule based on AT-percentage is generally competitive with other methods, 3.) in practice, the different methods will usually identify different sub-sequences as S/MARs, 4.) more research on the H-Rule would be valuable. CONCLUSION: A new insight is needed to design a method which will predict S/MARs well. Our data, including the control data, has been deposited as additional material and this may help later researchers test new predictors

Neutralisation of uPA with a Monoclonal Antibody Reduces Plasmin Formation and Delays Skin Wound Healing in tPA-Deficient Mice

Background: Proteolytic degradation by plasmin and metalloproteinases is essential for epidermal regeneration in skin wound healing. Plasminogen deficient mice have severely delayed wound closure as have mice simultaneously lacking the two plasminogen activators, urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA). In contrast, individual genetic deficiencies in either uPA or tPA lead to wound healing kinetics with no or only slightly delayed closure of skin wounds. Methodology/Principal Findings: To evaluate the therapeutic potential in vivo of a murine neutralizing antibody directed against mouse uPA we investigated the efficacy in skin wound healing of tPA-deficient mice. Systemic administration of the anti-mouse uPA monoclonal antibody, mU1, to tPA-deficient mice caused a dose-dependent delay of skin wound closure almost similar to the delayed kinetics observed in uPA;tPA double-deficient mice. Analysis of wound extracts showed diminished levels of plasmin in the mU1-treated tPA-deficent mice. Immunohistochemistry revealed that fibrin accumulated in the wounds of such mU1-treated tPA-deficent mice and that keratinocyte tongues were aberrant. Together these abnormalities lead to compromised epidermal closure. Conclusions/Significance: Our findings demonstrate that inhibition of uPA activity with a monoclonal antibody in adult tPA-deficient mice mimics the effect of simultaneous genetic ablation of uPA and tPA. Thus, application of the murin

Melkas innhold av fytoøstrogener påvirkes av engdriftsmåte og produksjonssystem

Engdriftsmåte som gir høyt innhold av rødkløver i grovfôret gir høyt innhold av equol i melk. Produksjonssystem hadde mindre betydning i denne studien

Viewpoints. 75 Sides to Freie Universität Berlin

Das Buch mit 75 Illustrationen und Kurztexten ist eine Reise durch die Geschichte und Welt der Freien Universität Berlin und entstand im April 2023 anlässlich ihres 75-jährigen Gründungsjubiläums. Die Bilder zeigen, ohne Anspruch auf Vollständigkeit, mit Augenzwinkern, eine vernetzte und internationale Universität, die sich seit ihren Anfängen und fortwährend ihren Leitwerten Freiheit, Gerechtigkeit und Wahrheit verpflichtet fühlt und zu einem bedeutenden Teil der Stadt und des Wissenschaftsraums Berlin geworden ist. Aspekte ihrer Geschichte und Gegenwart wurden künstlerisch interpretiert von Yves Haltner, einem Schweizer Illustrator und Grafiker, der in Berlin lebt und arbeitet.The book containing 75 illustrations and short texts is a journey through the history and world of Freie Universität Berlin. It was created in April 2023 to mark the occasion of the 75th anniversary of the university’s founding. With a hint of irony and pointed fragmentary playfulness, the images depict a connected, international university deeply committed to its core values: freedom, justice, truth. The university has become an important part of Berlin as a city and research hub. Various aspects of the past and present of the university were artistically interpreted by Yves Haltner, a Swiss illustrator and graphic designer who lives and works in Berlin

Weather risk models for prediction of septoria tritici blotch

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