Holistic Approach to Addressing Community Needs in Rural Communities

The purpose of this study was to assess health of a rural Texas community in efforts to better understand and develop a plan of action for developing community intervention for increasing availability of human resources. Researchers asked various questions to understand the availability and accessibility of resources within a rural community using a community needs assessment approach. Participants were recruited by the researchers directly within the community using purposive and snowball sampling techniques. The sample size included 361 participants in a rural community. The overall quality of life for the rural participants was slightly lower than what researchers considered to be healthy. Participants also indicated that the current resources within the rural community did not meet the needs for mental health (54%; n = 195), physical health (46.8%; n = 169), or social services (55.4%; n = 200). Implications for such findings suggest the need for strength-based collaboration and services informed by the communities themselves

The Efficacy of Dandelion Root Extract in Inducing Apoptosis in Drug-Resistant Human Melanoma Cells

Notoriously chemoresistant melanoma has become the most prevalent form of cancer for the 25–29 North American age demographic. Standard treatment after early detection involves surgical excision (recurrence is possible), and metastatic melanoma is refractory to immuno-, radio-, and most harmful chemotherapies. Various natural compounds have shown efficacy in killing different cancers, albeit not always specifically. In this study, we show that dandelion root extract (DRE) specifically and effectively induces apoptosis in human melanoma cells without inducing toxicity in noncancerous cells. Characteristic apoptotic morphology of nuclear condensation and phosphatidylserine flipping to the outer leaflet of the plasma membrane of A375 human melanoma cells was observed within 48 hours. DRE-induced apoptosis activates caspase-8 in A375 cells early on, demonstrating employment of an extrinsic apoptotic pathway to kill A375 cells. Reactive Oxygen Species (ROS) generated from DRE-treated isolated mitochondria indicates that natural compounds in DRE can also directly target mitochondria. Interestingly, the relatively resistant G361 human melanoma cell line responded to DRE when combined with the metabolism interfering antitype II diabetic drug metformin. Therefore, treatment with this common, yet potent extract of natural compounds has proven novel in specifically inducing apoptosis in chemoresistant melanoma, without toxicity to healthy cells

Avaliação da cinética de secagem de sementes de azevém (Lolium multiflorum L.).

SIEPE

Energetics of the primary electron transfer reaction revealed by ultrafast spectroscopy on modified bacterial reaction centers

The modification of reaction centers from Rhodobacter sphaeroides by the introduction of pheophytins instead of bacteriopheophytins leads to interesting changes in the primary photosynthetic reaction: long-living populations of the excited electronic state of the special pair P* and the bacteriochlorophyll anion B−A show up. The data allow the determination of the energetics in the reaction center. The free energy of the first intermediate P+B−A, where the electron has reached the accessory bacteriochlorophyll BA lies ≈ 450 cm−1 below the initially excited special pair P*