Cytotoxic and Genotoxic Effects of Sulfonamide-Aldehyde Derivative in Allium cepa Root Tip Cells

The continuous production and release into the environment of chemicals has revealed the need to determine their cytotoxicity and genotoxicity. Sulfonamide-aldehyde (SA) derivatives, whose biological activity properties vary in a wide spectrum, are frequently used in agriculture, medicine, pharmacy and many other fields. These compounds have an important cycle in the ecological system due to their use and diversity. In the present study, the potential cytotoxic and genotoxic effects of sulfonamide-aldehyde derivative were investigated using Allium test system with the concentrations of 6.25, 12.5, 25, 50, and 100 μM. In A. cepa cells, the increasing concentrations of SA caused cytotoxic effects by inducing nuclear lesions and inhibition of mitotic index. In addition, the increasing concentrations of SA caused genotoxic effects by inducing micronucleus and chromosome aberrations, which the most common ones are C-mitosis, sticky metaphase, and anaphase bridge. The results indicate that the concentration of 25 μM is EC50 in micronucleus, nuclear lesions, and chromosome aberrations; and 50 μM is EC50 in mitotic index (p lt; 0.05)

New chromosomal data, karyotype asymmetry and polyploid variations of some Gundelia (Asteraceae) species from Turkey

The genus Gundelia is currently represented with 18 species, of which 16 are in Turkey. In genus Gundelia, the chromosomal data were reported from 12 species. In the present study, it is aimed to eliminate the deficiencies in the knowledge about chromosomal data of Gundelia species. In Genus Gundelia, only a single chromosome number had been detected as 2n=18 so far. The chromosome numbers of four species were reported here for the first time: G. armeniaca, G. cappadocica, G. siirtica, and G. tehranica. In addition, the polyploidy in the genus was rare and G. anatolica was identified as the first polyploid species. All karyotypes except G. tehranica were symmetrical, consisting of metacentric and submetacentric chromosomes. Secondary constrictions were observed in the distal regions of the long arms of the longest metacentric and submetacentric chromosomes. Thus, the chromosomal data of all Turkish Gundelia species were completed. In conclusion, the present study presented new data into the karyological records relating the karyotype evolution and interspecific relations of genus Gundelia

Artiodactyla takımının familya ve türlerinin Karyotip Simetri/Asimetri indeksi (S/AI) ile karşılaştırılması

The S/AI formula is a parameter used to determine the karyotype asymmetry in higher animals and humans. The formula was performed to the 47 Artiodactyla species. According to the S/AI values between 1.0000 and 3.0000, the dendrograms were drawn demonstrating the interspecies and interfamilies relationships in Artiodactyla. The female karyotype types are between symmetric and asymmetric in the eight families, 18 genera and 36 species; symmetric in the five families, nine genera and 10 species; full symmetric in the only one family, genus and species. The male karyotype types are between symmetric and asymmetric in the 8 families, 18 genera and 34 species; symmetric in the five families, eight genera and nine species; full symmetric in the only one family, genus and species.The S/AI formula is a parameter used to determine the karyotype asymmetry in animals and humans. The formula was performed to the 47 Artiodactyla species. According to the S/AI values between 1.0000 and 3.0000, the dendrograms were drawn demonstrating the interspecies and interfamilies relationships in Artiodactyla. The female karyotype types are between symmetric and asymmetric in the eight families, 18 genera and 36 species; symmetric in the five families, nine genera and 10 species; full symmetric in the only one family, genus and species. The male karyotype types are between symmetric and asymmetric in the 8 families, 18 genera and 34 species; symmetric in the five families, eight genera and nine species; full symmetric in the only one family, genus and species

In vitro cytogenetic effects of Hypericum heterophyllum in human peripheral blood lymphocytes

Hypericum species have been used in traditional medicine in Turkey for thousands of years. In order to determine the cytogenetic effects of Hypericum heterophyllum, human lymphocytes were incubated with the aqueous extracts. Mitotic index frequencies and replication index values were increased with increasing extract concentrations of H. heterophyllum. The results indicate the cytotoxic effects as well as proliferative effects and suggest that the extracts of the compounds exhibit cytotoxic properties as well as mitotic and proliferative properties

Effects of Dosages and Application Periods of EDTA on Morphological and Cytogenetic Characters of Barley (Hordeum vulgare L.) Seedlings

Abstract:&nbsp;Ethylenediaminetetraacetic acid (EDTA) has many applications in plant research in addition to other applications, such as chelation and medical therapy. The morphological and cytogenetic effects of EDTA on barley seedlings were investigated in the present study. Seeds of Hordeum vulgare L. were treated with different doses of EDTA (30, 60, 90, 120 and 150 mmol/l) and 2 different application periods (20 and 22 h). Except mitotic index, all of the measurements for the investigated characters were carried out on days 7 and 14. EDTA treatments affected germination rates in nearly all treatments. In general, EDTA treatments decreased the root, leaf and coleoptile lengths, but it had the greatest effect on coleoptile length. Increasing the EDTA dosages and treatment periods also decreased the mitotic index compared to the untreated control. The inhibitory effects of EDTA dosages on the mitotic index indicate that EDTA can have genotoxic and mutagenic effects on barley seedlings.Keywords:&nbsp;EDTA, Hordeum vulgare, mitotic index</p

Genotoxic Effects of Hypericum heterophyllum Vent. in Human Lymphocytes Cultures

Abstract Hypericum species, growing wild in Anatolia, widely used in traditional medicine in Turkey. In order to determine the genotoxic effect of Hypericum heterophyllum, human lymphocytes were incubated with the aqueous extracts. The increasing extract concentrations of Hypericum heterophyllum induced micronucleus. The rise of micronucleus shows that Hypericum heterophyllum at high concentrations may become carcinogenic and genotoxic. The positive correlation was observed between micronucleus and age. Also, the positive correlation was observed between micronucleus rates of female and male. Further studies will be needed to determine the effects of the main bioactive components isolated from this species on micronucleus

Effects of the application of different concentrations of NaN3 for different times on the morphological and cytogenetic characteristics of barley (Hordeum vulgare L.) seedlings

Sodium azide (NaN3) has been used in many biological studies as a mutagen. In the present study, the morphological and cytogenetic effects of NaN3 on barley (Hordeum vulgare L.) seedlings were investigated. Seeds of barley were treated with different concentrations of NaN3 (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mmol/L) and applied for different periods of time Q and 4 h). Parameters investigated, except for the mitotic index, were determined on Days 7 and 14. Increasing concentrations of NaN3 affected germination rates on Days 7 and 14 following application for 3 and 4 h. Although the length of the roots and leaves was affected by treatment with NaN3 on Day 14 of the germination period, coleoptile length was affected by NaN3 treatment on Day 7. Increasing concentrations of NaN3 and increased treatment period decreased the mitotic index compared with the untreated control. The inhibitory effects of NaN3 on the mitotic index indicate that NaN3 can have genotoxic and mutagenic effects on barley seedlings