Zero order and area under curve spectrophotometric methods for determination of aspirin in pharmaceutical formulation

Objective: - A simple, accurate, precise and specific zero order and area under curve spectrophotometric methods has been developed for determination of Aspirin in its tablet dosage form by using methanol as a solvent.  Methods: - (1) Derivative Spectrophotometric Methods: - The amplitudes in the zero order derivative of the resultant spectra at 224 nm was selected to find out Aspirin in its tablet dosage form by using methanol as a solvent.(2) Area under curve (Area calculation): -The proposed area under curve method involves measurement of area at selected wavelength ranges. Two wavelength ranges were selected 218-227 nm for estimation of Aspirin.Result & Discussion: -The linearity was found to be 5-25 μg/ml for Aspirin. The mean % recoveries were found to be 99.47% and 100.43% of zero order derivative and area under curve method of Aspirin. For Repeatability, Intraday precision, Interday precision, % RSD were found to be 0.6416, 0.0046 and 0.9819, 0.8112 for zero order and 0.7257, 0.8731 and 0.7528, 1.7943 for area under curve method respectively. Limit of Detection and Quantitation were found to be 0.4823μg/ml and 1.5182μg/ml for zero order and 0.8272μg/ml and 2.5351μg/ml for area under curve method respectively. Assay results of market formulation were found to be 100.54% for zero order and 100.89% area under curve method respectively. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of Aspirin in its Tablet dosage form.Conclusion: - The developed methods can be concluded as accurate, sensitive and precise and can be easily applied to the pharmaceutical formulation