20 research outputs found
The origin of human pathogenicity and biological interactions in Chaetothyriales
Fungi in the order Chaetothyriales are renowned for their ability to cause human infections. Nevertheless, they are not
regarded as primary pathogens, but rather as opportunists with a natural habitat in the environment. Extremotolerance is
a major trend in the order, but quite diferent from black yeasts in Capnodiales which focus on endurance, an important
additional parameter is advancing toxin management. In the ancestral ecology of rock colonization, the association with
metabolite-producing lichens is signifcant. Ant-association, dealing with pheromones and repellents, is another mainstay
in the order. The phylogenetically derived family, Herpotrichiellaceae, shows dual ecology in monoaromatic hydrocarbon
assimilation and the ability to cause disease in humans and cold-blooded vertebrates. In this study, data on ecology, phylogeny, and genomics were collected and analyzed in order to support this hypothesis on the evolutionary route of the species of
Chaetothyriales. Comparing the ribosomal tree with that of enzymes involved in toluene degradation, a signifcant expansion
of cytochromes is observed and the toluene catabolism is found to be complete in some of the Herpotrichiellaceae. This
might enhance human systemic infection. However, since most species have to be traumatically inoculated in order to cause
disease, their invasive potential is categorized as opportunism. Only in chromoblastomycosis, true pathogenicity might be
surmised. The criterion would be the possible escape of agents of vertebrate disease from the host, enabling dispersal of
adapted genotypes to subsequent generations.info:eu-repo/semantics/publishedVersio
Comparative effectiveness of initial computed tomography and invasive coronary angiography in women and men with stable chest pain and suspected coronary artery disease: multicentre randomised trial
To assess the comparative effectiveness of computed tomography and invasive coronary angiography in women and men with stable chest pain suspected to be caused by coronary artery disease
Endoscopic third ventriculostomy in patients with slit ventricle syndrome or altered anatomy of the third ventricle
Hand in Hand: Experimental and Theoretical Investigations into the Reactions of Copper(I) Mono- and Bis(guanidine) Complexes with Dioxygen
Zinc Chloride Complexes with Aliphatic and Aromatic Guanidine Hybrid Ligands and Their Activity in the Ring-Opening Polymerisation of d,l-Lactide
Thermodynamic parameters at bio-nano interface and nanomaterial toxicity: A case study on BSA interaction with ZnO, SiO2 and TiO2
Understanding nanomaterial (NM)–protein interactions is a key issue in defining the bioreactivity of NMs with great impact for nanosafety. In the present work, the complex phenomena occurring at the bio/nano interface were evaluated in a simple case study focusing on NM–protein binding thermodynamics and protein stability for three representative metal oxide NMs, namely, zinc oxide (ZnO; NM-110), titanium dioxide (TiO2; NM-101), and silica (SiO2; NM-203). The thermodynamic signature associated with the NM interaction with an abundant protein occurring in most cell culture media, bovine serum albumin (BSA), has been investigated by isothermal titration and differential scanning calorimetry. Circular dichroism spectroscopy offers additional information concerning adsorption-induced protein conformational changes. The BSA adsorption onto NMs is enthalpy-controlled, with the enthalpic character (favorable interaction) decreasing as follows: ZnO (NM-110) > SiO2 (NM-203) > TiO2 (NM-101). The binding of BSA is spontaneous, as revealed by the negative free energy, ΔG, for all systems. The structural stability of the protein decreased as follows: TiO2 (NM-101) > SiO2 (NM-203) > ZnO (NM-110). As protein binding may alter NM reactivity and thus the toxicity, we furthermore assessed its putative influence on DNA damage, as well as on the expression of target genes for cell death (RIPK1, FAS) and oxidative stress (SOD1, SOD2, CAT, GSTK1) in the A549 human alveolar basal epithelial cell line. The enthalpic component of the BSA–NM interaction, corroborated with BSA structural stability, matched the ranking for the biological alterations, i.e., DNA strand breaks, oxidized DNA lesions, cell-death, and antioxidant gene expression in A549 cells. The relative and total content of BSA in the protein corona was determined using mass-spectrometry-based proteomics. For the present case study, the thermodynamic parameters at bio/nano interface emerge as key descriptors for the dominant contributions determining the adsorption processes and NMs toxicological effect
The HEP@NET study: an internet-based registry examining the efficacy of heparin coating in patients undergoing coronary stent implantation
Thermodynamic Parameters at BioNano Interface and Nanomaterial Toxicity: A Case Study on BSA Interaction with ZnO SiO2, and TiO2
Name Changes in Medically Important Fungi and Their Implications for Clinical Practice
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