A study of school plant needs and a new high school for Revere, Massachusetts

Thesis (M.Arch.) Massachusetts Institute of Technology. Dept. of Architecture, 1954.Accompanying drawings held by MIT Museum.Bibliography: leaves [134]-136.by Thorkel Myron W. Haaland.M.Arch

Hyperspectral microarray scanning: impact on the accuracy and reliability of gene expression data

BACKGROUND: Commercial microarray scanners and software cannot distinguish between spectrally overlapping emission sources, and hence cannot accurately identify or correct for emissions not originating from the labeled cDNA. We employed our hyperspectral microarray scanner coupled with multivariate data analysis algorithms that independently identify and quantitate emissions from all sources to investigate three artifacts that reduce the accuracy and reliability of microarray data: skew toward the green channel, dye separation, and variable background emissions. RESULTS: Here we demonstrate that several common microarray artifacts resulted from the presence of emission sources other than the labeled cDNA that can dramatically alter the accuracy and reliability of the array data. The microarrays utilized in this study were representative of a wide cross-section of the microarrays currently employed in genomic research. These findings reinforce the need for careful attention to detail to recognize and subsequently eliminate or quantify the presence of extraneous emissions in microarray images. CONCLUSION: Hyperspectral scanning together with multivariate analysis offers a unique and detailed understanding of the sources of microarray emissions after hybridization. This opportunity to simultaneously identify and quantitate contaminant and background emissions in microarrays markedly improves the reliability and accuracy of the data and permits a level of quality control of microarray emissions previously unachievable. Using these tools, we can not only quantify the extent and contribution of extraneous emission sources to the signal, but also determine the consequences of failing to account for them and gain the insight necessary to adjust preparation protocols to prevent such problems from occurring

Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102

Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array. A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium. Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology. Due in part to the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains. One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition

Strengthening patient-centred communication in rural Ugandan health centres: A theory-driven evaluation within a cluster randomized trial.

This article describes a theory-driven evaluation of one component of an intervention to improve the quality of health care at Ugandan public health centres. Patient-centred services have been advocated widely, but such approaches have received little attention in Africa. A cluster randomized trial is evaluating population-level outcomes of an intervention with multiple components, including 'patient-centred services.' A process evaluation was designed within this trial to articulate and evaluate the implementation and programme theories of the intervention. This article evaluates one hypothesized mechanism of change within the programme theory: the impact of the Patient Centred Services component on health-worker communication. The theory-driven approach extended to evaluation of the outcome measures. The study found that the proximal outcome of patient-centred communication was rated 10 percent higher (p < 0.008) by care seekers consulting with the health workers who were at the intervention health centres compared with those at control health centres. This finding will strengthen interpretation of more distal trial outcomes

Laser acceleration of ion beams

We consider methods of charged particle acceleration by means of high-intensity lasers. As an application we discuss a laser booster for heavy ion beams provided, e.g. by the Dubna nuclotron. Simple estimates show that a cascade of crossed laser beams would be necessary to provide additional acceleration to gold ions of the order of GeV/nucleon.Comment: 4 pages, 4 figures, Talk at the Helmholtz International Summer School "Dense Matter in heavy Ion Collisions and Astrophysics", August 21 - September 1, 2006, JINR Dubna, Russia; v2, misprints correcte

Estimation of cold plasma outflow during geomagnetic storms

Low-energy ions of ionospheric origin constitute a significant contributor to the magnetospheric plasma population. Measuring cold ions is difficult though. Observations have to be done at sufficiently high altitudes and typically in regions of space where spacecraft attain a positive charge due to solar illumination. Cold ions are therefore shielded from the satellite particle detectors. Furthermore, spacecraft can only cover key regions of ion outflow during segments of their orbit, so additional complications arise if continuous longtime observations, such as during a geomagnetic storm, are needed. In this paper we suggest a new approach, based on a combination of synoptic observations and a novel technique to estimate the flux and total outflow during the various phases of geomagnetic storms. Our results indicate large variations in both outflow rates and transport throughout the storm. Prior to the storm main phase, outflow rates are moderate, and the cold ions are mainly emanating from moderately sized polar cap regions. Throughout the main phase of the storm, outflow rates increase and the polar cap source regions expand. Furthermore, faster transport, resulting from enhanced convection, leads to a much larger supply of cold ions to the near-Earth region during geomagnetic storms. ©2015. The Authors

Prediction and understanding of soft proton contamination in XMM-Newton: a machine learning approach

One of the major and unfortunately unforeseen sources of background for the current generation of X-ray telescopes are few tens to hundreds of keV (soft) protons concentrated by the mirrors. One such telescope is the European Space Agency's (ESA) X-ray Multi-Mirror Mission (XMM-Newton). Its observing time lost due to background contamination is about 40\%. This loss of observing time affects all the major broad science goals of this observatory, ranging from cosmology to astrophysics of neutron stars and black holes. The soft proton background could dramatically impact future large X-ray missions such as the ESA planned Athena mission (http://www.the-athena-x-ray-observatory.eu/). Physical processes that trigger this background are still poorly understood. We use a Machine Learning (ML) approach to delineate related important parameters and to develop a model to predict the background contamination using 12 years of XMM observations. As predictors we use the location of satellite, solar and geomagnetic activity parameters. We revealed that the contamination is most strongly related to the distance in southern direction, $Z$, (XMM observations were in the southern hemisphere), the solar wind radial velocity and the location on the magnetospheric magnetic field lines. We derived simple empirical models for the first two individual predictors and an ML model which utilizes an ensemble of the predictors (Extra Trees Regressor) and gives better performance. Based on our analysis, future missions should minimize observations during times associated with high solar wind speed and avoid closed magnetic field lines, especially at the dusk flank region in the southern hemisphere.Comment: 20 pages, 11 figure

Heavy Metal and Rock in Space: Cluster RAPID Observations of Fe and Si

Metallic and silicate ions carry essential information about the evolution of the Earth and near-Earth small bodies. Despite this, there has so far been very little focus on ions with atomic masses higher than oxygen in the terrestrial magnetosphere. In this paper, we report on abundances and properties of energetic ions with masses corresponding to that of silicon (Si) and iron (Fe) in Earth's geospace. The results are based on a newly derived data product from the Research with Adaptive Particle Imaging Detectors on Cluster. We find traces of both Si and Fe in all of the regions covered by the spacecraft, with the highest occurrence rates and highest intensities in the inner magnetosphere. We also find that the Fe and Si abundances are modulated by solar activity. During solar maximum, the probability of observing Fe and Si in geospace increases significantly. On the other hand, we find little or no direct correlation between geomagnetic activity and Si and Fe abundance in the magnetosphere. Both Si and Fe in the Earth's magnetosphere are inferred to be primarily of solar wind origin.publishedVersio

High‐density magnetospheric He+ at the dayside magnetopause and its effect on magnetic reconnection

Observations from the Magnetospheric Multiscale (MMS) mission are used to quantify the maximum effect of magnetospheric H+ and He+ on dayside magnetopause reconnection. A data base of current-sheet crossings from the first 2 years of the MMS mission is used to identify magnetopause crossings with the highest He+ concentrations. While all of these magnetopause crossings exhibit evidence of plasmaspheric plume material, only half of the crossings are directly associated with plasmaspheric plumes. The He+ density varies dramatically within the magnetosphere adjacent to the magnetopause, with density variations of an order of magnitude on timescales as short as 10 s, the time resolution of the composition instrument on MMS. Plasma wave observations are used to determine the total electron density, and composition measurements are used to determine the mass density in the magnetosheath and magnetosphere. These mass densities are then used with the magnetic field observations to determine the theoretical reduction in the reconnection rate at the magnetopause. The presence of high-density plasmaspheric plume material at the magnetopause causes transient reductions in the reconnection rate of up to ∼40%.publishedVersio