36 research outputs found
A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles
AbstractExosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that do not consider the heterogeneity found within exosomal subpopulations. Here, we present a nanoscale cytometry platform NanoEPIC, enabling phenotypic sorting and exoPD-L1 profiling from blood plasma. We highlight the efficacy of NanoEPIC in monitoring anti-PD-1 immunotherapy through the interrogation of exoPD-L1. NanoEPIC generates signature exoPD-L1 patterns in responders and non-responders. In mice treated with PD1-targeted immunotherapy, exoPD-L1 is correlated with tumor growth, PD-L1 burden in tumors, and the immune suppression of CD8+ tumor-infiltrating lymphocytes. Small extracellular vesicles (sEVs) with different PD-L1 expression levels display distinctive inhibitory effects on CD8 + T cells. NanoEPIC offers robust, high-throughput profiling of exosomal markers, enabling sEV subpopulation analysis. This platform holds the potential for enhanced cancer screening, personalized treatment, and therapeutic response monitoring.</jats:p
Temperature effect on performance of nanoparticle/surfactant flooding in enhanced heavy oil recovery
Capillary-Assisted Molecular Pendulum Bioanalysis
The development of robust biosensing strategies that
can be easily
implemented in everyday life remains a challenge for the future of
modern biosensor research. While several reagentless approaches have
attempted to address this challenge, they often achieve user-friendliness
through sacrificing sensitivity or universality. While acceptable
for certain applications, these trade-offs hinder the widespread adoption
of reagentless biosensing technologies. Here, we report a novel approach
to reagentless biosensing that achieves high sensitivity, rapid detection,
and universality using the SARS-CoV-2 virus as a model target. Universality
is achieved by using nanoscale molecular pendulums, which enables
reagentless electrochemical biosensing through a variable antibody
recognition element. Enhanced sensitivity and rapid detection are
accomplished by incorporating the coffee-ring phenomenon into the
sensing scheme, allowing for target preconcentration on a ring-shaped
electrode. Using this approach, we obtained limits of detection of
1 fg/mL and 20 copies/mL for the SARS-CoV-2 nucleoproteins and viral
particles, respectively. In addition, clinical sample analysis showed
excellent agreement with Ct values from PCR-positive SARS-CoV-2 patients