Demasqué der diversificatie

De recente geruchtmakende opsplitsingen van enkele vooraanstaande concerns zijn vooral gepresenteerd als acties die voortkomen uit zorg voor de aandeelhouderswaarde. In deze bijdrage wordt gesteld, dat zij eerder het gevolg zijn van het spaak lopen van al te uitbundige acquisitiestrategieën uit het verleden. De negatieve effecten daarvan konden gedurende lange tijd worden gecompenseerd door een flinke dosis marktmacht. Door toenemende mondiale concurrentie is deze marktmacht afgenomen. Aan de noodzakelijk geworden herstructureringen wordt helaas opnieuw vorm gegeven door middel van fusies en acquisities

Use of genome-scale metabolic models for plant metabolism

Genome-scale metabolic models (GSMM) have been extensively applied for a wide variety of organisms. However, for plants genome-scale modeling is still in its infancy. It has been successfully developed for Arabidopsis in the last few years. In this study we appraise the existing GSMM for Arabidopsis by computing biomass accumulation using flux balance analysis (FBA) and attempt to outline potential applications for horticultural crops in the future. The predicted growth rates for the Poolman et al. (2009) and Dal’Molin et al. (2010) model were quite similar, despite different carbon sources were provided (glucose and sucrose,100 mmol/g*h DW). The exchange fluxes for respiration in nonphotosynthetic cells confirmed that plants prefer NH3 in the process of nitrogen assimilation. When carbohydrates were used as substrate for respiration the Respiratory quotient (RQ) of Poolman’s model approached 1, which is consistent with experimental data. For the Dal’Molin’s model the RQ is less than 1. H2S is utilized in the Dal’Molin model as sulfur source, whereas sulfate (SO42-) is consumed in the Poolman’s model. The Poolman’s model seems to reflect biological reality more closely than the Dal’Molin model. This could be due to the subcellular compartmentation included in the Dal’Molin model, adding subcellular compartmentation of reactions was done manually. In conclusion, to apply constraints-based reconstruction and analysis (COBRA) methods, including FBA, to plant metabolism requires careful analysis and possibly curation of existing GSMM’s