Detection of Temper Embrittlement in Steel by Magnetoacoustic Emssion Technique

A bulk ferromagnet possesses two types of domain walls: 180° and non-180° [1]. In the case of iron-like ferromagnets, the latter type of walls are 90° domain walls. As a result of the magnetoelastic interaction, unit cells of a ferromagnet deform slightly in a way that is unique to particular types of domains [2]. Such a spontaneous deformation, called magnetostriction, causes local lattice strains at domain walls with the strain fields being particularly strong for 90° domain walls [3]. The motion of the 90° domain walls is followed by a redistribution of local lattice strain fields. Elastic energy is being released by this process and propagates through material as acoustic waves. Acoustic emission (AE) generated due to magnetic domain wall motion is thus defined as magnetoacoustic emission (MAE)

Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis

Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.link_to_subscribed_fulltex