Streptococcus pneumoniae Serotype 1 Capsular Polysaccharide Induces CD8+CD28− Regulatory T Lymphocytes by TCR Crosslinking

Zwitterionic capsular polysaccharides (ZPS) of commensal bacteria are characterized by having both positive and negative charged substituents on each repeating unit of a highly repetitive structure that has an α-helix configuration. In this paper we look at the immune response of CD8+ T cells to ZPSs. Intraperitoneal application of the ZPS Sp1 from Streptococcus pneumoniae serotype 1 induces CD8+CD28− T cells in the spleen and peritoneal cavity of WT mice. However, chemically modified Sp1 (mSp1) without the positive charge and resembling common negatively charged polysaccharides fails to induce CD8+CD28− T lymphocytes. The Sp1-induced CD8+CD28− T lymphocytes are CD122lowCTLA-4+CD39+. They synthesize IL-10 and TGF-β. The Sp1-induced CD8+CD28− T cells exhibit immunosuppressive properties on CD4+ T cells in vivo and in vitro. Experimental approaches to elucidate the mechanism of CD8+ T cell activation by Sp1 demonstrate in a dimeric MHC class I-Ig model that Sp1 induces CD8+ T cell activation by enhancing crosslinking of TCR. The expansion of CD8+CD28− T cells is independent, of direct antigen-presenting cell/T cell contact and, to the specificity of the T cell receptor (TCR). In CD8+CD28− T cells, Sp1 enhances Zap-70 phosphorylation and increasingly involves NF-κB which ultimately results in protection versus apoptosis and cell death and promotes survival and accumulation of the CD8+CD28− population. This is the first description of a naturally occurring bacterial antigen that is able to induce suppressive CD8+CD28− T lymphocytes in vivo and in vitro. The underlying mechanism of CD8+ T cell activation appears to rely on enhanced TCR crosslinking. The data provides evidence that ZPS of commensal bacteria play an important role in peripheral tolerance mechanisms and the maintenance of the homeostasis of the immune system

Acquired and congenital disorders of sung performance: A review.

Many believe that the majority of people are unable to carry a tune. Yet, this widespread idea underestimates the singing abilities of the layman. Most occasional singers can sing in tune and in time, provided that they perform at a slow tempo. Here we characterize proficient singing in the general population and identify its neuronal underpinnings by reviewing behavioral and neuroimaging studies. In addition, poor singing resulting from a brain injury or neurogenetic disorder (i.e., tone deafness or congenital amusia) is examined. Different lines of evidence converge in indicating that poor singing is not a monolithic deficit. A variety of poor-singing "phenotypes" are described, with or without concurrent perceptual deficits. In addition, particular attention is paid to the dissociations between specific abilities in poor singers (e.g., production of absolute vs. relative pitch, pitch vs. time accuracy). Such diversity of impairments in poor singers can be traced to different faulty mechanisms within the vocal sensorimotor loop, such as pitch perception and sensorimotor integration

Noise Annoyance Caused by Amplitude Modulated Sounds Resembling the Main Characteristics of Temporal Wind Turbine Noise

Annoyance ratings for artificially created noises, resembling the main characteristics of temporal wind turbine noise, were studied by means of a listening experiment involving 21 participants with normal hearing. Three types of stimuli were examined: broadband noise (−4 dB/octave), noise generated by moving cars, and narrowband noise. All stimuli had the sound level fluctuations typical for wind turbine noise. The magnitude of the sound level fluctuations was measured in a quantitative way, by using the characteristics of amplitude modulated sound: modulation rate and modulation depth. Our aim was to examine how the modulation rate and the modulation depth influence the noise annoyance assessment of broadband and narrowband amplitude modulated noises. Three different modulation rates, 1, 2 and 4 Hz, and sound level fluctuations (a measure of the modulation depth), 3, 6, 9 dB, were applied to each type of stimuli (with exception of noise generated by the moving cars) and investigated. The participants in the listening experiment were presented with sound stimuli in laboratory conditions and asked to rate their annoyance on a numerical scale. The results have shown a significant difference between the investigated conditions. The effect was particularly strong between the annoyance judgments of different types of noise (narrow and broadband), and modulated versus unmodulated noises. Temporal fluctuations occurring in wind turbine noise are very pertinent to the perception of annoyance and could be responsible for its being a relatively annoying noise source. The obtained results were discussed and compared to the typical modulation rates and level changes that occur in recordings of real wind turbine noise

Pitch Processing of Speech : Comparison of Psychoacoustic and Electrophysiological Data

The present study consisted of two experiments. The goal of the first experiment was to establish the just noticeable differences for the fundamental frequency of the vowel /u/ by using the 2AFC method. We obtained the threshold value for 27 cents. This value is larger than the motor reaction values which had been observed in previous experiments (e.g. 9 or 19 cents). The second experiment was intended to provide neurophysiological confirmation of the detection of shifts in a frequency, using event-related potentials (ERPs). We concentrated on the mismatch negativity (MMN) - the component elicited by the change in the pattern of stimuli. Its occurrence is correlated with the discrimination threshold. In our study, MMN was observed for changes greater than 27 cents - shifts of ±50 and 100 cents (effect size - Cohen’s d = 2.259). MMN did not appear for changes of ±10 and 20 cents. The results showed that the values for which motor responses can be observed are indeed lower than those for perceptual thresholds

Equal autophonic level curves under different room acoustics conditions

The indirect auditory feedback from one's own voice arises from sound reflections at the room boundaries or from sound reinforcement systems. The relative variations of indirect auditory feedback are quantified through room acoustic parameters such as the room gain and the voice support, rather than the reverberation time. Fourteen subjects matched the loudness level of their own voice (the autophonic level) to that of a constant and external reference sound, under different synthesized room acoustics conditions. The matching voice levels are used to build a set of equal autophonic level curves. These curves give an indication of the amount of variation in voice level induced by the acoustic environment as a consequence of the sidetone compensation or Lombard effect. In the range of typical rooms for speech, the variations in overall voice level that result in a constant autophonic level are on the order of 2 dB, and more than 3 dB in the 4 kHz octave band. By comparison of these curves with previous studies, it is shown that talkers use acoustic cues other than loudness to adjust their voices when speaking in different rooms. (C) 2011 Acoustical Society of America. [DOI: 10.1121/1.3598429]status: publishe

Sieve Element Ca2+ Channels as Relay Stations between Remote Stimuli and Sieve Tube Occlusion in Vicia faba[W]

Damage induces remote occlusion of sieve tubes in Vicia faba by forisome dispersion, triggered during the passage of an electropotential wave (EPW). This study addresses the role of Ca2+ channels and cytosolic Ca2+ elevation as a link between EPWs and forisome dispersion. Ca2+ channel antagonists affect the initial phase of the EPW as well as the prolonged plateau phase. Resting levels of sieve tube Ca2+ of ∼50 nM were independently estimated using Ca2+-selective electrodes and a Ca2+-sensitive dye. Transient changes in cytosolic Ca2+ were observed in phloem tissue in response to remote stimuli and showed profiles similar to those of EPWs. The measured elevation of Ca2+ in sieve tubes was below the threshold necessary for forisome dispersion. Therefore, forisomes need to be associated with Ca2+ release sites. We found an association between forisomes and endoplasmic reticulum (ER) at sieve plates and pore-plasmodesma units where high-affinity binding of a fluorescent Ca2+ channel blocker mapped an increased density of Ca2+ channels. In conclusion, propagation of EPWs in response to remote stimuli is linked to forisome dispersion through transiently high levels of parietal Ca2+, release of which depends on both plasma membrane and ER Ca2+ channels