182 research outputs found
Reupholstering at home
28 pages; includes photograph, drawings and plans. This archival publication may not reflect current scientific knowledge or recommendations. Current information available from the University of Minnesota Extension: https://www.extension.umn.edu
Real-Gas Effects and Phase Separation in Underexpanded Jets at Engine-Relevant Conditions
A numerical framework implemented in the open-source tool OpenFOAM is
presented in this work combining a hybrid, pressure-based solver with a
vapor-liquid equilibrium model based on the cubic equation of state. This
framework is used in the present work to investigate underexpanded jets at
engine-relevant conditions where real-gas effects and mixture induced phase
separation are probable to occur. A thorough validation and discussion of the
applied vapor-liquid equilibrium model is conducted by means of general
thermodynamic relations and measurement data available in the literature.
Engine-relevant simulation cases for two different fuels were defined. Analyses
of the flow field show that the used fuel has a first order effect on the
occurrence of phase separation. In the case of phase separation two different
effects could be revealed causing the single-phase instability, namely the
strong expansion and the mixing of the fuel with the chamber gas. A comparison
of single-phase and two-phase jets disclosed that the phase separation leads to
a completely different penetration depth in contrast to single-phase injection
and therefore commonly used analytical approaches fail to predict the
penetration depth.Comment: Preprint submitted to AIAA Scitech 2018, Kissimmee, Florid
PELATIHAN CODING MENGGUNAKAN SCRATCH KEPADA SISWA-SISWI SD NEGERI 100 MALUKU TENGAH
The purpose of this activity is to explore the effectiveness of coding training using Scratch for students of Elementary School (SD) Negeri 100 Central Maluku. The training method is carried out through a series of sessions designed to introduce basic programming concepts and the use of Scratch as a visual programming tool. This research involved students of SD Negeri 100 Central Maluku as the subject of this activity. Data was collected through pre and post training tests, as well as participatory observation during training sessions. The results showed that coding training using Scratch was effective in increasing students' understanding of programming concepts and their ability to create interactive projects using Scratch. In addition, this training also improves students' critical thinking skills, creativity, and cooperation. These findings provide strong support for the introduction of coding using Scratch at the elementary education level and emphasize the importance of integrating programming into the school curriculu
Tear fluid biomarkers in ocular and systemic disease: potential use for predictive, preventive and personalised medicine
In the field of predictive, preventive and personalised medicine, researchers are keen to identify novel and reliable ways to predict and diagnose disease, as well as to monitor patient response to therapeutic agents. In the last decade alone, the sensitivity of profiling technologies has undergone huge improvements in detection sensitivity, thus allowing quantification of minute samples, for example body fluids that were previously difficult to assay. As a consequence, there has been a huge increase in tear fluid investigation, predominantly in the field of ocular surface disease. As tears are a more accessible and less complex body fluid (than serum or plasma) and sampling is much less invasive, research is starting to focus on how disease processes affect the proteomic, lipidomic and metabolomic composition of the tear film. By determining compositional changes to tear profiles, crucial pathways in disease progression may be identified, allowing for more predictive and personalised therapy of the individual. This article will provide an overview of the various putative tear fluid biomarkers that have been identified to date, ranging from ocular surface disease and retinopathies to cancer and multiple sclerosis. Putative tear fluid biomarkers of ocular disorders, as well as the more recent field of systemic disease biomarkers, will be shown
Unravelling enzymatic discoloration in potato through a combined approach of candidate genes, QTL, and expression analysis
Enzymatic discoloration (ED) of potato tubers was investigated in an attempt to unravel the underlying genetic factors. Both enzyme and substrate concentration have been reported to influence the degree of discoloration and as such this trait can be regarded as polygenic. The diploid mapping population C × E, consisting of 249 individuals, was assayed for the degree of ED and levels of chlorogenic acid and tyrosine. Using this data, Quantitative Trait Locus (QTL) analysis was performed. Three QTLs for ED have been found on parental chromosomes C3, C8, E1, and E8. For chlorogenic acid a QTL has been identified on C2 and for tyrosine levels, a QTL has been detected on C8. None of the QTLs overlap, indicating the absence of genetic correlations between these components underlying ED, in contrast to earlier reports in literature. An obvious candidate gene for the QTL for ED on Chromosome 8 is polyphenol oxidase (PPO), which was previously mapped on chromosome 8. With gene-specific primers for PPO gene POT32 a CAPS marker was developed. Three different alleles (POT32-1, -2, and -3) could be discriminated. The segregating POT32 alleles were used to map the POT32 CAPS marker and QTL analysis was redone, showing that POT32 coincides with the QTL peak. A clear correlation between allele combinations and degree of discoloration was observed. In addition, analysis of POT32 gene expression in a subset of genotypes indicated a correlation between the level of gene expression and allele composition. On average, genotypes having two copies of allele 1 had both the highest degree of discoloration as well as the highest level of POT32 gene expression
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Multiphase phenomena in Diesel fuel injection systems
Fuel Injection Equipment (FIE) are an integral component of modern Internal Combustion Engines (ICE), since they play a crucial role in the fuel atomization process and in the formation of a fuel/air combustible mixture, consequently affecting efficiency and pollutant formation. Advancements and improvements of FIE systems are determined by the complexity of the physical mechanisms taking place; the spatial scales are in the order of millimetres, flow may become locally highly supersonic, leading to very small temporal scales of microseconds or less. The operation of these devices is highly unsteady, involving moving geometries such as needle valves. Additionally, extreme pressure changes imply that many assumptions of traditional fluid mechanics, such as incompressibility, are no longer valid. Furthermore, the description of the fuel properties becomes an issue, since fuel databases are scarce or limited to pure components, whereas actual fuels are commonly hydrocarbon mixtures. Last but not least, complicated phenomena such as phase change or transition from subcritical to transcritical/supercritical state of matter further pose complications in the understanding of the operation of these devices
Cancer Biomarker Discovery: The Entropic Hallmark
Background: It is a commonly accepted belief that cancer cells modify their transcriptional state during the progression of the disease. We propose that the progression of cancer cells towards malignant phenotypes can be efficiently tracked using high-throughput technologies that follow the gradual changes observed in the gene expression profiles by employing Shannon's mathematical theory of communication. Methods based on Information Theory can then quantify the divergence of cancer cells' transcriptional profiles from those of normally appearing cells of the originating tissues. The relevance of the proposed methods can be evaluated using microarray datasets available in the public domain but the method is in principle applicable to other high-throughput methods. Methodology/Principal Findings: Using melanoma and prostate cancer datasets we illustrate how it is possible to employ Shannon Entropy and the Jensen-Shannon divergence to trace the transcriptional changes progression of the disease. We establish how the variations of these two measures correlate with established biomarkers of cancer progression. The Information Theory measures allow us to identify novel biomarkers for both progressive and relatively more sudden transcriptional changes leading to malignant phenotypes. At the same time, the methodology was able to validate a large number of genes and processes that seem to be implicated in the progression of melanoma and prostate cancer. Conclusions/Significance: We thus present a quantitative guiding rule, a new unifying hallmark of cancer: the cancer cell's transcriptome changes lead to measurable observed transitions of Normalized Shannon Entropy values (as measured by high-throughput technologies). At the same time, tumor cells increment their divergence from the normal tissue profile increasing their disorder via creation of states that we might not directly measure. This unifying hallmark allows, via the the Jensen-Shannon divergence, to identify the arrow of time of the processes from the gene expression profiles, and helps to map the phenotypical and molecular hallmarks of specific cancer subtypes. The deep mathematical basis of the approach allows us to suggest that this principle is, hopefully, of general applicability for other diseases
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