The influence of cadmium stress on the content of mineral nutrients and metal-binding proteins in arabidopsis halleri

We investigated the influence of cadmium stress on zinc hyperaccumulation, mineral nutrient uptake, and the content of metal-binding proteins in Arabidopsis halleri. The experiments were carried out using plants subjected to long-term cadmium exposure (40 days) in the concentrations of 45 and 225 μM Cd2+. Inductively coupled plasma-mass spectrometry, size exclusion chromatography coupled with plasma-mass spectrometry, and laser ablation inductively coupled plasma-mass spectrometry used for ablation of polyacylamide gels were employed to assess the content of investigated elements in plants as well as to identify metal-binding proteins. We found that A. halleri is able to translocate cadmium to the aerial parts in high amounts (translocation index >1). We showed that Zn content in plants decreased significantly with the increase of cadmium content in the growth medium. Different positive and negative correlations between Cd content and mineral nutrients were evidenced by our study. We identified more than ten low-molecular-weight (<100 kDa) Cd-binding proteins in Cd-treated plants. These proteins are unlikely to be phytochelatins or metallothioneins. We hypothesize that low-molecular-weight Cd-binding proteins can be involved in cadmium resistance in A. halleri

Is metal extraction by Arabidopsis halleri related to exchangeable metal rates in soils amended with different metal-bearing solids?

International audienc

Strategies of heavy metal uptake by three plant species growing near a metal smelter

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Etude de trois especes vegetales metallophytes : quantification, localisation et speciation d'elements metalliques (Zn, Pb, Cu, Cd) a differentes echelles Evaluation de la faisabilite d'application a la rehabilitation de sols pollues

Available from INIST (FR), Document Supply Service, under shelf-number : RP 400 (2360) / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEAgence de l'Environnement et de la Maitrise de l'Energie (ADEME), 75 - Paris (France)FRFranc

Plants growing in abandoned mines of Portugal are useful for biogeochemical exploration of arsenic, antimony, tungsten and mine reclamation

Several plants across taxonomic hierarchy have evolved heavy metal tolerance strategies and detoxification mechanisms that enable them to survive, grow and reproduce in metal contaminated and polluted sites. Plants growing on the abandoned Portuguese mines, highly contaminated with arsenic (As), antimony (Sb) and tungsten (W), have been studied for their biogeochemical prospecting and mine stabilization potential. The results of soil analysis show relevant anomalies of As, Sb and W. We have observed that the plant species accumulating tungsten are Digitalis purpurea, Chamaespartium tridentatum, Cistus ladanifer, Pinus pinaster, Erica umbellata, and Quercus ilex subsp. ballota. Accumulators of antimony are D. purpurea, E. umbellata, Calluna vulgaris and C. ladanifer. Accumulations of arsenic are found in the old needles of P. pinaster, Calluna vulgaris and C. tridentatum and leaves of C. ladanifer, E. umbellate and Q. ilex subsp. ballota. These are the key stone species allowing biogeochemical delineation of areas of anomalous soil composition.http://www.sciencedirect.com/science/article/B6VCP-4F2B7FJ-1/1/c84dc459b909e6a87cb30a17d940fac

Do Arabidopsis halleri from nonmetallicolous populations accumulate zinc and cadmium more effectively than those from metallicolous populations?

The ability of metallicolous and nonmetallicolous populations of Arabidopsis halleri to accumulate zinc (Zn), cadmium (Cd) and lead (Pb) is compared here in order to explore the extent and variability of this trait in wild A. halleri plants. Aerial plant parts and the soil around the harvested plants were collected and analysed for metal concentrations or total and extractable metal concentrations, respectively, for 20 metallicolous and 13 nonmetallicolous populations. Results show that metallicolous and nonmetallicolous populations have the same ability to accumulate Zn and Cd but that neither population type is able to accumulate Pb. Between populations within type, an homogenous accumulating response is observed for Zn, whereas the ability to accumulate Cd is variable. Zn and Cd accumulation to very high concentrations is a constitutive property of the species. The Zn and Cd hyperaccumulator trait of A. halleri from contaminated sites was confirmed. Interestingly, nonmetallicolous plants are Zn and Cd hyperaccumulators. The possibility of using A. halleri in phytoremediation is discussed.SCOPUS: ar.jFLWINinfo:eu-repo/semantics/publishe