5 research outputs found
The complete chloroplast genome of Cinnamomum camphora (L.) Presl., a unique economic plant to China
Cinnamomum camphora (Lauraceae) Presl. is a unique economic plant to China. The complete chloroplast (cp) genome was sequenced and assembled by using Illumina paired-end reads data. The circular cp genome is 152,729 bp in size, including a pair of inverted repeat (IRs) regions of 20,074 bp, a large single copy (LSC) region of 93,688 bp and a small single copy (SSC) region of 18,893 bp. The genome contains 127 unique genes, including 83 protein-coding genes (PCGs), 36 transfer RNA genes (tRNAs), and 8 ribosomal RNA genes (rRNAs). Besides, 19 genes possess a single intron, while another three genes (ycf3, rps12, and clpP) have a couple of introns. The GC content of entire C. camphora cp genome, LSC, SSC, and IR regions are 39.2, 38.0, 33.9, and 44.4%, respectively. Phylogenetic analysis based on the concatenated coding sequences of cp PCGs showed that C. camphora and Cinnamomum verum are closely related with each other within the genus of cinnamomum
In vitro propagation of Camellia fascicularis: a plant species with extremely small populations
Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary buds per nodal stem were obtained from C. fascicularis cultured on Murashige and Skoog (MS) medium containing 0.1 mg/L of indole-3-acetic acid (IAA) combined with 1.0 of mg/L 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to the modified woody plant medium (WPM) supplemented with 3.0 mg/L of BA in combination with 0.3 mg/L of IAA for multiplication, thereby resulting in the highest shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on 1/2 MS medium supplemented with 2.0 mg/L of indole-3-butyric acid (IBA) in combination with 0.3 mg/L of α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing root of nodal stems was 1.0 mg/L of IBA in combination with 2.0 mg/L of NAA, which produced 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
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Uncommon Behavior of Li Doping Suppresses Oxygen Redox in P2‐Type Manganese‐Rich Sodium Cathodes
Utilizing both cationic and anionic oxygen redox reactions is regarded as an important approach to exploit high-capacity layered cathode materials with earth abundant elements. It has been popular strategies to effectively elevate the oxygen redox activities by Li-doping to introduce unhybridized O 2p orbitals in Nax MnO2 -based chemistries or enabling high covalency transition metals in P2-Na0.66 Mnx TM1- x O2 (TM = Fe, Cu, Ni) materials. Here, the effect of Li doping on regulating the oxygen redox activities P2-structured Na0.66 Ni0.25 Mn0.75 O2 materials is investigated. Systematic X-ray characterizations and ab initio simulations have shown that the doped Li has uncommon behavior in modulating the density of states of the neighboring Ni, Mn, and O, leading to the suppression of the existing oxygen and Mn redox reactivities and the promotion of the Ni redox. The findings provide a complementary scenario to current oxygen redox mechanisms and shed lights on developing new routes for high-performance cathodes