Ability of Lactobacillus fermentum to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides

<p>Abstract</p> <p>Background</p> <p>Soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissular α-galactosidase in mammals. The passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. The aim of the work reported here was to assess the ability of α-galactosidase-producing lactobacilli to improve the digestibility of α-galacto-oligosaccharides <it>in situ</it>.</p> <p>Results</p> <p>Gnotobiotic rats were orally fed with soy milk and placed in respiratory chambers designed to monitor fermentative gas excretion. The validity of the animal model was first checked using gnotobiotic rats monoassociated with a <it>Clostridium butyricum </it>hydrogen (H₂)-producing strain. Ingestion of native soy milk by these rats caused significant H₂emission while ingestion of α-galacto-oligosaccharide-free soy milk did not, thus validating the experimental system. When native soy milk was fermented using the α-galactosidase-producing <it>Lactobacillus fermentum </it>CRL722 strain, the resulting product failed to induce H₂emission in rats thus validating the bacterial model. When <it>L. fermentum </it>CRL722 was coadministered with native soy milk, a significant reduction (50 %, <it>P </it>= 0.019) in H₂emission was observed, showing that α-galactosidase from <it>L. fermentum </it>CRL722 remained active <it>in situ</it>, in the gastrointestinal tract of rats monoassociated with <it>C. butyricum</it>. In human-microbiota associated rats, <it>L. fermentum </it>CRL722 also induced a significant reduction of H₂emission (70 %, <it>P </it>= 0.004).</p> <p>Conclusion</p> <p>These results strongly suggest that <it>L. fermentum </it>α-galactosidase is able to partially alleviate α-galactosidase deficiency in rats. This offers interesting perspectives in various applications in which lactic acid bacteria could be used as a vector for delivery of digestive enzymes in man and animals.</p

Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides-0

Olled with a magnetic valve; d, silica gel-containing cylinder to trap moisture; e, KOH-containing cylinder to trap CO; f, peristaltic pump; g, sampling port with syringe. Arrows indicate the direction of air flow through the system.<p><b>Copyright information:</b></p><p>Taken from "Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides"</p><p>http://www.biomedcentral.com/1471-2180/8/22</p><p>BMC Microbiology 2008;8():22-22.</p><p>Published online 29 Jan 2008</p><p>PMCID:PMC2270848.</p><p></p

Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides-1

<p><b>Copyright information:</b></p><p>Taken from "Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides"</p><p>http://www.biomedcentral.com/1471-2180/8/22</p><p>BMC Microbiology 2008;8():22-22.</p><p>Published online 29 Jan 2008</p><p>PMCID:PMC2270848.</p><p></p

Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides-4

Hambers were analyzed for Hconcentration using GC. Treatments successively applied to all rats (for more details, see Material and Methods section and Fig. 5) are boxed. Cross bars indicate standard error of the mean. MW, metabolic weight; *, indicates that values (Student-Newman-Keuls test) of the differences with the group receiving soy milk is < 0.01.<p><b>Copyright information:</b></p><p>Taken from "Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides"</p><p>http://www.biomedcentral.com/1471-2180/8/22</p><p>BMC Microbiology 2008;8():22-22.</p><p>Published online 29 Jan 2008</p><p>PMCID:PMC2270848.</p><p></p

Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides-2

water. , Rats associated with DSM10702; , rats associated with a human faecal microbiota; , α-GOS-enriched soy milk (see and ) was added with a 5 % (v/v) coffee bean α-Gal solution at 1 IU/ml and was incubated for 12 h at 37°C; , the concentrations of raffinose and stachyose in the α-GOS-enriched soy milk administered to Cb rats were 48 and 76 mM, respectively; , the concentrations of raffinose and stachyose in the α-GOS-enriched soy milk administered to HMA rats were 144 and 228 mM, respectively; , cultures were at 4 × 10CFU/ml; , α-GOS-enriched soy milk (see ) was added with a 2 % (v/v) inoculum of a 4 × 10CFU/ml CRL722 suspension and was allowed to ferment for 16 h at 37°C.<p><b>Copyright information:</b></p><p>Taken from "Ability of to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides"</p><p>http://www.biomedcentral.com/1471-2180/8/22</p><p>BMC Microbiology 2008;8():22-22.</p><p>Published online 29 Jan 2008</p><p>PMCID:PMC2270848.</p><p></p