Polydopamine-Decorated Microcomposites Promote Functional Recovery of an Injured Spinal Cord by Inhibiting Neuroinflammation

Neuroinflammation following spinal cord injury usually aggravates spinal cord damage. Many inflammatory cytokines are key players in neuroinflammation. Owing largely to the multiplicity of cytokine targets and the complexity of cytokine interactions, it is insufficient to suppress spinal cord damage progression by regulating only one or a few cytokines. Herein, we propose a two-pronged strategy to simultaneously capture the released cytokines and inhibit the synthesis of new ones in a broad-spectrum manner. To achieve this strategy, we designed a core/shell-structured microcomposite, which was composed of a methylprednisolone-incorporated polymer inner core and a biocompatible polydopamine outer shell. Thanks to the inherent adhesive nature of polydopamine, the obtained microcomposite (MP-PLGA@PDA) efficiently neutralized the excessive cytokines in a broad-spectrum manner within 1 day after spinal cord injury. Meanwhile, the controlled release of immunosuppressive methylprednisolone reduced the secretion of new inflammatory cytokines. Benefiting from its efficient and broad-spectrum capability in reducing the level of cytokines, this core/shell-structured microcomposite suppressed the recruitment of macrophages and protected the injured spinal cord, leading to an improved recovery of motor function. Overall, the designed microcomposite successfully achieved the two-pronged strategy in cytokine neutralization, providing an alternative approach to inhibit neuroinflammation in the injured spinal cord.Peer reviewe

Prevalence and Determinants of Metabolic Syndrome among Women in Chinese Rural Areas

BACKGROUND AND AIMS: Metabolic syndrome (MS) is prevalent in recent years but few data is reported in the rural areas in China. The aim of this study was to examine MS prevalence and its risk factors among women in rural China. METHODS AND RESULTS: The Nantong Metabolic Syndrome Study (NMSS), a population based cross-sectional study, was conducted during 2007-2008 in Nantong, China. In person interviews, blood glucose and lipid measurements were completed for 13,505 female participants aged 18-74 years. The International Diabetes Federation (IDF), the US Third Report of the National Cholesterol Education Program, the Adult Treatment Panel (ATPIII) and modified ATPIII for Asian population has determined three criteria of MS. These criteria for MS were used and compared in this study. The prevalence of MS was 22.0%, 16.9% and 23.3% according to IDF, ATPIII and ATPIII-modified criteria, respectively. Levels of agreement of these criteria for MS were above 0.75. We found that vigorous-intensity of occupational physical activity was associated with a low prevalence of MS with OR of 0.76 (95% confidence interval (CI): 0.63-0.91). Rice wine drinkers (alcohol >12.8 g/day) had about 34% low risks of developing MS with OR of 0.66 (95% CI: 0.48-0.91), compared with non-drinkers. Odds ratio of MS was 1.81 (95% CI: 1.15-2.84) in women who smoked more than 20 pack-years, compared to non-smokers. Odds ratio of MS was 1.56 (95% CI: 1.25-1.95) in women who had familial history of diseases, including hypertension, diabetes and stroke, compared to women without familial history of those diseases. CONCLUSION: MS is highly prevalent among women in rural China. Both physical activity and rice wine consumption play a protective role, while family history and smoking are risk factors in MS development. Educational programs should be established for promoting healthy lifestyles and appropriate interventions in rural China

Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in Fusarium verticillioides

Fusarium verticillioides is the major maize pathogen associated with ear rot and stalk rot worldwide. Fumonisin B1 (FB1) produced by F. verticillioides, poses a serious threat to human and animal health. However, our understanding of FB1 synthesis and virulence mechanism in this fungus is still very limited. Glycosylation catalyzed by glycosyltransferases (GTs) has been identified as contributing to fungal infection and secondary metabolism synthesis. In this study, a family 2 glycosyltransferase, FvCpsA, was identified and characterized in F. verticillioides. ΔFvcpsA exhibited significant defects in vegetative growth. Moreover, ΔFvcpsA also increased resistance to osmotic and cell wall stress agents. In addition, expression levels of FUM genes involved in FB1 production were greatly up-regulated in ΔFvcpsA. HPLC (high performance liquid chromatography) analysis revealed that ΔFvcpsA significantly increased FB1 production. Interestingly, we found that the deletion of FvCPSA showed penetration defects on cellophane membrane, and thus led to obvious defects in pathogenicity. Characterization of FvCpsA domain experiments showed that conserved DXD and QXXRW domains were vital for the biological functions of FvCpsA. Taken together, our results indicate that FvCpsA is critical for fungal growth, FB1 biosynthesis and virulence in F. verticillioides

Investigation on the mechanical and tribological properties of polyethylene wax/silicone rubber composites

As an auxiliary agent for rubber processing, polyethylene wax can be used to enhance the diffusion of filler in rubber, improve the extrusion speed of rubber compound and facilitate rubber demoulding, which has good development potential in the field of rubber processing and manufacturing. At present, there is no research on the optimum amount of polyethylene wax in silicone rubber. Therefore, in this paper, we studied the influence of different amount of polyethylene wax on the mechanical and friction properties of silicone rubber. Firstly, silicone rubber composites with different polyethylene wax content were prepared by mechanical mixing and hot pressing. Then, the mechanical properties and friction and wear properties of silicone rubber composites were tested by tensile testing machine and multifunctional comprehensive tester for surface properties of materials. Finally, combined with a series of characterization methods, such as three-dimensional (3D) morphology, scanning electron microscopy (SEM), microscopic observation and energy dispersive X-ray spectroscopy (EDS), the mechanism of polyethylene wax in silicone rubber was studied. The results show that the best addition amount of polyethylene wax in silicone rubber is 0.25 phr, and the friction coefficient of polyethylene wax/silicone rubber composite is the lowest and most stable, and the wear amount is the least. In the process of friction, polyethylene wax can be separated from silicone rubber matrix and distributed on the surface of wear marks, playing a better role in lubrication and wear reduction

Research on Methods of Active Steering Control Based on Receding Horizon Control

Active steering technology is a key technology for automatic driving vehicles to achieve route tracking and obstacle avoidance and risk avoidance, and its performance will affect the stability control of the vehicle. For solving the stability control issues of vehicles, which have uncertainty in model and robustness in system, this paper proposes an active steering control method based on the receding horizon control model. It calculates the optimal control law by this method by using the real-time vehicle state so that it can compensate for the uncertainty caused by model mismatch, interference, etc. The design of the controller is implemented by using the yaw rate deviation of the vehicle as the input of the receding horizon linear quadratic controller model and then inputting the calculated superposition angle into the vehicle model in real time. We built a Simulink control model to implement co-simulation with CarSim to verify the control effect of the controller. In addition, we built a steering hardware-in-the-loop platform based on the LabVIEW RT system. The experimental results show that the active steering system adopting a receding horizon control method had better system robustness and robust stability

EPS Current Tracking Method Research Based on Hybrid Sensitivity H∞ Control Algorithm

For electric power steering system (EPS), road interference, noise of the sensor, and the uncertainty of the steering system may make EPS control effect and the driver's road sense worse. EPS system which takes advantage of good current tracking ability, good anti-interference ability, and good operation stability is becoming more and more important in automotive research. The traditional H∞ control algorithm can solve the system uncertainty theoretically, but it cannot solve the contradiction between robustness and performance without considering the performance of the system. Therefore, this paper proposes a EPS current tracking method based on the hybrid sensitivity H∞ control algorithm, which takes the current tracking performance as one of the control objectives, so that the system can maximize the robustness and performance. Firstly, the dynamic model of EPS is established. Then, the two-degree-of-freedom vehicle model and tire model are introduced. The state space equation of the system is constructed on the basis of the system state space with random disturbance signals, the hybrid sensitivity H∞ controller is designed in the sensitivity index design, and the proposed algorithm can use weighting function to minimize the performance of the current tracking error as well as the robustness of the yaw rate error in response to robustness. Simulation analysis and experimental verification of EPS system are also carried out. The results show that the control method of the hybrid sensitivity H∞ can better achieve EPS target current tracking, effectively suppress the effect of external interference and noise, improve the system performance and robustness, ensure the driver get good road sense, and improve the system of steering stability

A Novel Reactive Power Optimization in Distribution Network Based on Typical Scenarios Partitioning and Load Distribution Matching Method

This paper proposed an entropy weight optimum seeking method (EWOSM) based on the typical scenarios partitioning and load distribution matching, to solve the reactive power optimization problem in distribution network under the background of big data. Firstly, the mathematic model of reactive power optimization is provided to analyze the relationship between the data source and the optimization schemes in distribution network, which illustrate the feasibility of using large amount of historical data to solve reactive power optimization. Then, the typical scenarios partitioning method and load distribution matching method are presented, which can select out some loads that have the same or similar distributions with the load to be optimized from historical database rapidly, and the corresponding historical optimization schemes are used as the alternatives. As the reactive power optimization is a multi-objective problem, the multi-attribute decision making method based on entropy weight method is used to select out the optimal scheme from the alternatives. The objective weights of evaluation indexes are determined by entropy weight method, and then the multi-attribute decision making problem is transformed to a single attribute decision making problem. Finally, the proposed method is tested on several systems with different scales and compared with existing methods to prove the validity and superiority

Study of Semi-Dry High Target Solidification/Stabilization of Harmful Impurities in Phosphogypsum by Modification

Phosphogypsum (PG) treatment is one of the research hotspots in the field of environmental protection. Many researchers both at home and abroad have devoted themselves to studies on harmless resource treatment of PG, but the treatment technology is unable to meet the demand of PG consumption due to the huge production and storage demands. In order to solve the problem of PG pollution, this study explored the different solidified effects of various modification formulations on the hazardous components in PG, using industrial solid waste calcium carbide slag (CCS) as an alkaline regulator; Portland cement (PC), polyaluminum chloride (PAC) and CaCl2 as the main raw materials of the solidification and stabilization formula and the water content in PG as the reaction medium. The results showed that CCS (0.5%), PC (0.4%) and PAC (0.3%) had a more significant solidified effect on phosphorus (P) and fluoride (F). PAC was added in two steps and reacted under normal temperature and pressure, and its leaching toxicity meets the requirements of relevant standards, which laid an excellent foundation for PG-based ecological restoration materials and filling materials, with low economic cost, simple process and strong feasibility. This will provide great convenience for the later mining and metallurgy

Liquid–liquid phase separation of H3K27me3 reader BP1 regulates transcriptional repression

Abstract Background Bromo-adjacent homology-plant homeodomain domain containing protein 1 (BP1) is a reader of histone post-translational modifications in fungi. BP1 recognizes trimethylation of lysine 27 in histone H3 (H3K27me3), an epigenetic hallmark of gene silencing. However, whether and how BP1 participates in transcriptional repression remains poorly understood. Results We report that BP1 forms phase-separated liquid condensates to modulate its biological function in Fusarium graminearum. Deletion assays reveal that intrinsically disordered region 2 (IDR2) of BP1 mediates its liquid–liquid phase separation. The phase separation of BP1 is indispensable for its interaction with suppressor of Zeste 12, a component of polycomb repressive complex 2. Furthermore, IDR2 deletion abolishes BP1-H3K27me3 binding and alleviates the transcriptional repression of secondary metabolism-related genes, especially deoxynivalenol mycotoxin biosynthesis genes. Conclusions BP1 maintains transcriptional repression by forming liquid–liquid phase-separated condensates, expanding our understanding of the relationship between post-translational modifications and liquid–liquid phase separation