Towards the identification of the soil microbiome community associated with Longleaf Pine

This project is part of a larger study looking at the restoration of the Long-leaf Pine ecosystem in certain Wildlife Management Areas (WMA) in Northwest Georgia. As part of this larger study another group of researchers has been looking at changes in the plant community in the same plots used for this soil microbiome research. Our long-term aim is to look for potential associations between the above and below-ground community structures. Although research has shown that the health and composition of the microbiome surrounding the roots of plants has a significant impact on the ability of plants to fight and survive various stressors (Wei et al. 2019), the full extent of the complex system of feedback mechanisms between the rhizosphere and soil microbiome, and the above ground plant communities is not yet understood. We collected soil samples from six plots located in the Sheffield WMA located in Paulding County, GA. Except for two plots at the savanna site, with slopes south or west facing; soil samples were collected from either north or south facing hillsides. Within each plot, samples were collected in sterile plastic tubes at the center of the plot and 10 m above and below from the center. Five random sub samples were taken from each tube for DNA extraction using a commercially available kit. Given that plant communities appear to differ between north and south facing slopes, we hypothesize that the soil microbiome will also differ significantly in north versus south facing areas. We also hypothesize that the microbiome in the savanna plots, which are actively being restored for Longleaf pine, will be distinct from all others

Prototype Biodiversity Digital Twin: honey bees in agricultural landscapes

Honey bees are vital to human well-being and are under multiple stresses. We need to be able to assess the viability and productivity of honey bee colonies in different landscapes and under different management and climate-change scenarios. We have developed a prototype digital twin, HONEYBEE-pDT, based on the BEEHAVE model, which simulates foraging, population dynamics and Varroa mite infestation of a single honey bee colony. The main input data are land-cover maps and daily weather data. We have developed the pDT for simulating large areas and have tested it for the whole of Germany. We have also developed a web-based GUI that users can use to run the pDT for specific sites. Hive weight data from hundreds of hives will be used for calibration and validation

Réseautage en Psychologie en Belgique

editorial reviewed4. Quality educatio

The Lantern Vol. 1, No. 1, May 1933

• Remember: Translation of Rappelle-toi by Alfred de Musset • Lighting the Lantern • Footfalls • To a Lovely Lady • The Sons of Martha • Strategy • Lumine Lunae • Poetry in Retrospect • Nirvana • A Domestic Episode • At Night • Haman and Hitler • This is What He Said • Bookocracy • Four Loves • Cities and Personalitieshttps://digitalcommons.ursinus.edu/lantern/1000/thumbnail.jp

Beyond bouncing back? Comparing and contesting urban resilience frames in US and Latin American contexts

Urban resilience has gained considerable popularity in planning and policy to address cities’ capacity to cope with climate change. While many studies discuss the different ways that academics define resilience, little attention has been given to how resilience is conceptualized across different urban contexts and among the actors that engage in building resilience ‘on the ground’. Given the implications that resilience frames can have for the solutions that are pursued (and who benefits from them), it is important to examine how transformative definitions of urban resilience are in practice. In this paper, we use data from a survey of nine US and Latin American and Caribbean cities to explore how the concept is framed across multiple governance sectors, including governmental, non-governmental, business, research, and hybrid organizations. We examine these framings in light of recent conceptual developments and tensions found in the literature. The results highlight that, in general across the nine cities, framings converge with definitions of resilience as the ability to resist, cope with, or bounce back to previous conditions, whereas sustainability, equity, and social-ecological-technological systems (SETS) perspectives are rarely associated with resilience. There are noticeable differences across cities and governance actors that point to geographic and political variation in the way resilience is conceptualized. We unpack these differences and discuss their implications for resilience research and practice moving forward. We argue that if resilience is going to remain a major goal for city policies into the future, it needs to be conceived in a more transformative, anticipatory, and equitable way, and acknowledge interconnected SETS

MapSplice: Accurate Mapping of RNA-Seq Reads for Splice Junction Discovery

The accurate mapping of reads that span splice junctions is a critical component of all analytic techniques that work with RNA-seq data. We introduce a second generation splice detection algorithm, MapSplice, whose focus is high sensitivity and specificity in the detection of splices as well as CPU and memory efficiency. MapSplice can be applied to both short (\u3c75 bp) and long reads (≥75 bp). MapSplice is not dependent on splice site features or intron length, consequently it can detect novel canonical as well as non-canonical splices. MapSplice leverages the quality and diversity of read alignments of a given splice to increase accuracy. We demonstrate that MapSplice achieves higher sensitivity and specificity than TopHat and SpliceMap on a set of simulated RNA-seq data. Experimental studies also support the accuracy of the algorithm. Splice junctions derived from eight breast cancer RNA-seq datasets recapitulated the extensiveness of alternative splicing on a global level as well as the differences between molecular subtypes of breast cancer. These combined results indicate that MapSplice is a highly accurate algorithm for the alignment of RNA-seq reads to splice junctions. Software download URL: http://www.netlab.uky.edu/p/bioinfo/MapSplice

GLIS1 regulates trabecular meshwork function and intraocular pressure and is associated with glaucoma in humans.

Chronically elevated intraocular pressure (IOP) is the major risk factor of primary open-angle glaucoma, a leading cause of blindness. Dysfunction of the trabecular meshwork (TM), which controls the outflow of aqueous humor (AqH) from the anterior chamber, is the major cause of elevated IOP. Here, we demonstrate that mice deficient in the Krüppel-like zinc finger transcriptional factor GLI-similar-1 (GLIS1) develop chronically elevated IOP. Magnetic resonance imaging and histopathological analysis reveal that deficiency in GLIS1 expression induces progressive degeneration of the TM, leading to inefficient AqH drainage from the anterior chamber and elevated IOP. Transcriptome and cistrome analyses identified several glaucoma- and extracellular matrix-associated genes as direct transcriptional targets of GLIS1. We also identified a significant association between GLIS1 variant rs941125 and glaucoma in humans (P = 4.73 × 1