Foreign Languages in Poland - a Shift in Perspectives and Motivations

As a result of the fall of communism, opening to the West and the recent inauguration to the European Union, Polish people’s perspectives and consequently motivations to learn foreign languages have changed. After World War II, because of Soviet superintendence, Russian dominated as the primary second language in Poland. Learners had little or no say in their choices of language education. Now, two decades later, the situation is quite different. The following is a brief report on the current state of foreign languages in Poland, with a focus on the rise of a new first foreign language: English.特集 海外の語学教育事

コミュニケーションが元で 団結的な英語カリキュラムへ

The English curriculum at Muroran Institute of Technology has gone through many changes over the last few years. Many improvements have been seen but there are still parts of it that need refinement. This paper looks at a number of areas that will be researched in the hope that the curriculum can continue to develop and progress. In particular we look at four areas: reasoning behind the materials we use; how we assess materials; creation of an e-learning system; and the Common European Framework of Reference.教育改善報

HSP60 possesses a GTPase activity and mediates protein folding with HSP10

The mammalian molecular chaperone, HSP60, plays an essential role in protein homeostasis through mediating protein folding and assembly. The structure and ATP-dependent function of HSP60 has been well established in recent studies. After ATP, GTP is the major cellular nucleotide. In this paper, we have investigated the role of GTP in the activity of HSP60. It was found that HSP60 has different properties with respect to allostery, complex formation and protein folding activity depending on the nucleoside triphosphate present. The presence of GTP slightly affected the ATPase activity of HSP60 during protein folding. These results provide clues as to the functional mechanism of the HSP60-HSP10 complex

Geranylgeranylacetone selectively binds to the HSP70 of Helicobacter pylori and alters its coccoid morphology

Geranylgeranylacetone (GGA) is used to treat patients suffering from peptic ulcers and gastritis. We examined the effect of GGA on Helicobacter pylori, which is a causative factor of gastrointestinal diseases. Previously, we have reported that GGA binds specifically to the molecular chaperone HSP70. In this paper, we report that GGA bounds to H. pylori HSP70 (product of the DnaK gene) with 26-times higher affinity than to human HSP70, and induced large conformational changes as observed from surface plasmon resonance and circular dichroism. Binding of GGA suppressed the activity of the H. pylori chaperone. GGA also altered several characteristics of H. pylori cells. GGA-treated cells elicited enhanced interleukin-8 production by gastric cancer cell lines and potentiated susceptibility to complement as compared to untreated cells. GGA also caused morphological alterations in H. pylori as reflected in fewer coccoid-like cells, suggesting that GGA converts H. pylori to an actively dividing, spiral state (vegetative form) from a non-growing, coccoid state. This morphological conversion by GGA resulted in accelerated growth of H. pylori. These results suggest a model in which GGA sensitizes H. pylori to antibiotic treatment by converting the cells to an actively growing state

Physicochemical Properties of the Mammalian Molecular Chaperone HSP60

The E. coli GroEL/GroES chaperonin complex acts as a folding cage by producing a bullet-like asymmetric complex, and GroEL exists as double rings regardless of the presence of adenosine triphosphate (ATP). Its mammalian chaperonin homolog, heat shock protein, HSP60, and co-chaperonin, HSP10, play an essential role in protein folding by capturing unfolded proteins in the HSP60/HSP10 complex. However, the structural transition in ATPase-dependent reaction cycle has remained unclear. We found nucleotide-dependent association and dissociation of the HSP60/HSP10 complex using various analytical techniques under near physiological conditions. Our results showed that HSP60 exist as a significant number of double-ring complexes (football- and bullet-type complexes) and a small number of single-ring complexes in the presence of ATP and HSP10. HSP10 binds to HSP60 in the presence of ATP, which increased the HSP60 double-ring formation. After ATP is hydrolyzed to Adenosine diphosphate (ADP), HSP60 released the HSP10 and the dissociation of the double-ring to single-rings occurred. These results indicated that HSP60/HSP10 undergoes an ATP-dependent transition between the single- and double-rings in their system that is highly distinctive from the GroEL/GroES system particularly in the manner of complex formation and the roles of ATP binding and hydrolysis in the reaction cycle

The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90

The aryl hydrocarbon receptor is a member of the nuclear receptor superfamily that associates with the molecular chaperone HSP90 in the cytoplasm. The activation mechanism of the AhR is not yet fully understood. It has been proposed that after binding of ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3methylcholanthrene (3-MC), or β-naphthoflavone (β-NF), the AhR dissociates from HSP90 and translocates to the nucleus. It has also been hypothesized that the AhR translocates to the nucleus and forms a complex with HSP90 and other co-chaperones. There are a few reports about the direct association or dissociation of AhR and HSP90 due to difficulties in purifying AhR. We constructed and purified the PAS domain from AhR. Binding of the AhR-PAS domain to β-NF affinity resin suggested that it possesses ligand-binding affinity. We demonstrated that the AhR-PAS domain binds to HSP90 and the association is not affected by ligand binding. The ligand 17-DMAG inhibited binding of HSP90 to GST-PAS. In an immunoprecipitation assay, HSP90 was co-immunoprecipitated with AhR both in the presence or absence of ligand. Endogenous AhR decreased in the cytoplasm and increased in the nucleus of HeLa cells 15. min after treatment with ligand. These results suggested that the ligand-bound AhR is translocated to nucleus while in complex with HSP90.We used an in situ proximity ligation assay to confirm whether AhR was translocated to the nucleus alone or together with HSP90. HSP90 was co-localized with AhR after the nuclear translocation. It has been suggested that the ligand-bound AhR was translocated to the nucleus with HSP90. Activated AhR acts as a transcription factor, as shown by the transcription induction of the gene CYP1A1 8. h after treatment with β-NF

Historicism and constructionism: rival ideas of historical change

Simon ZB. Historicism and constructionism: rival ideas of historical change. History of European Ideas. 2019;45(8):1171-1190.A seemingly unitary appeal to history might evoke today two incompatible operations of historicization that yield contradictory results. This article attempts to understand two co-existing senses of historicity as conflicting ideas of historical change and rival practices of temporal comparison: historicism and constructionism. At their respective births, both claimed to make sense of the world and ourselves as changing over time. Historicism, dominating nineteenth-century Western thought and overseeing the professionalization of historical studies, advocated an understanding of the present condition of the human world as developing out of past conditions. Constructionism, dominating the second half of the twentieth century, understood the present condition as the recent invention of certain ‘historical’ environments, without prior existence. As competing ideas of historical change, they both entail a comparison between past and present conditions of their investigated subjects, but their practices of temporal comparison are irreconcilable and represent two distinct ways of historicization

A 10-step Analysis of Cultural Encounters - a tool to broaden the cultural understanding of Japanese students

This article proposes a 10-step analysis of an encounter with another culture as a tool to guide Japanese students to better awareness or understanding of cultural differences, the first step in the process of becoming a truly international person. The analysis focuses on one specific event, in which students’ expectations of particular behavior or response were not met because their interlocutor was from a different culture. The procedure prevents stereotyping and judgements of what is right or wrong, promotes self-awareness of cultural rules that govern their own behavior and finally instills understanding why people from other cultures do the things the way they do