Getting to Common Ground: A Comparison of Ontario, Canada's Provincial Policy Statement and the Auckland Council Regional Policy Statement with Respect to Indigenous Peoples

Indigenous rights are crucial to contemporary land use planning and policy in settler states. This article comparatively analyzes the manifest and latent content of the 2014 Provincial Policy Statement of Ontario, Canada (PPS) and the 1999 Auckland Council Regional Policy Statement of Aotearoa New Zealand (ACRPS) in order to evaluate their relative capacity to recognize the rights of Indigenous peoples. While the results show that jurisdiction is an impediment to fostering common ground between Indigenous peoples and settler states, the authors conclude that the PPS and the ACRPS serve vital roles in building dialogue and equitable planning outcomes

Getting to Common Ground: A Comparison of Ontario, Canada’s Provincial Policy Statement and the Auckland Council Regional Policy Statement with Respect to Indigenous Peoples

Indigenous rights are crucial to contemporary land use planning and policy in settler states. This article comparatively analyzes the manifest and latent content of the 2014 Provincial Policy Statement of Ontario, Canada (PPS) and the 1999 Auckland Council Regional Policy Statement of Aotearoa New Zealand (ACRPS) in order to evaluate their relative capacity to recognize the rights of Indigenous peoples. While the results show that jurisdiction is an impediment to fostering common ground between Indigenous peoples and settler states, the authors conclude that the PPS and the ACRPS serve vital roles in building dialogue and equitable planning outcomes

Derivation and Characterization of Induced Pluripotent Stem Cells from Equine Fibroblasts

Pluripotent stem cells offer unprecedented potential not only for human medicine but also for veterinary medicine, particularly in relation to the horse. Induced pluripotent stem cells (iPSCs) are particularly promising, as they are functionally similar to embryonic stem cells and can be generated in vitro in a patient-specific manner. In this study, we report the generation of equine iPSCs from skin fibroblasts obtained from a foal and reprogrammed using viral vectors coding for murine Oct4, Sox2, c-Myc, and Klf4 sequences. The reprogrammed cell lines were morphologically similar to iPSCs reported from other species and could be stably maintained over more than 30 passages. Immunostaining and polymerase chain reaction analyses revealed that these cell lines expressed an array of endogenous markers associated with pluripotency, including OCT4, SOX2, NANOG, REX1, LIN28, SSEA1, SSEA4, and TRA1-60. Furthermore, under the appropriate conditions, the equine iPSCs readily formed embryoid bodies and differentiated in vitro into cells expressing markers of ectoderm, mesoderm, and endoderm, and when injected into immunodeficient mice, gave raise to tumors containing differentiated derivatives of the 3 germ layers. Finally, we also reprogrammed fibroblasts from a 2-year-old horse. The reprogrammed cells were similar to iPSCs derived from neonatal fibroblasts in terms of morphology, expression of pluripotency markers, and differentiation ability. The generation of these novel cell lines constitutes an important step toward the understanding of pluripotency in the horse, and paves the way for iPSC technology to potentially become a powerful research and clinical tool in veterinary biomedicine

Improved detection of Pneumocystis jirovecii in upper and lower respiratory tract specimens from children with suspected pneumocystis pneumonia using real-time PCR: a prospective study

<p>Abstract</p> <p>Background</p> <p><it>Pneumocystis </it>pneumonia (PCP) is a major cause of hospitalization and mortality in HIV-infected African children. Microbiologic diagnosis relies predominantly on silver or immunofluorescent staining of a lower respiratory tract (LRT) specimens which are difficult to obtain in children. Diagnosis on upper respiratory tract (URT) specimens using PCR has been reported useful in adults, but data in children are limited. The main objectives of the study was (1) to compare the diagnostic yield of PCR with immunofluorescence (IF) and (2) to investigate the usefulness of upper compared to lower respiratory tract samples for diagnosing PCP in children.</p> <p>Methods</p> <p>Children hospitalised at an academic hospital with suspected PCP were prospectively enrolled. An upper respiratory sample (nasopharyngeal aspirate, NPA) and a lower respiratory sample (induced sputum, IS or bronchoalveolar lavage, BAL) were submitted for real-time PCR and direct IF for the detection of <it>Pneumocystis </it><it>jirovecii</it>. A control group of children with viral lower respiratory tract infections were investigated with PCR for PCP.</p> <p>Results</p> <p>202 children (median age 3.3 [inter-quartile range, IQR 2.2 - 4.6] months) were enrolled. The overall detection rate by PCR was higher than by IF [180/349 (52%) vs. 26/349 (7%) respectively; p < 0.0001]. PCR detected more infections compared to IF in lower respiratory tract samples [93/166 (56%) vs. 22/166 (13%); p < 0.0001] and in NPAs [87/183 (48%) vs. 4/183 (2%); p < 0.0001]. Detection rates by PCR on upper (87/183; 48%) compared with lower respiratory tract samples (93/166; 56%) were similar (OR, 0.71; 95% CI, 0.46 - 1.11). Only 2/30 (6.6%) controls were PCR positive.</p> <p>Conclusion</p> <p>Real-time PCR is more sensitive than IF for the detection of <it>P. jirovecii </it>in children with PCP. NPA samples may be used for diagnostic purposes when PCR is utilised. Wider implementation of PCR on NPA samples is warranted for diagnosing PCP in children.</p

Germline Transgenic Pigs by Sleeping Beauty Transposition in Porcine Zygotes and Targeted Integration in the Pig Genome

Genetic engineering can expand the utility of pigs for modeling human diseases, and for developing advanced therapeutic approaches. However, the inefficient production of transgenic pigs represents a technological bottleneck. Here, we assessed the hyperactive Sleeping Beauty (SB100X) transposon system for enzyme-catalyzed transgene integration into the embryonic porcine genome. The components of the transposon vector system were microinjected as circular plasmids into the cytoplasm of porcine zygotes, resulting in high frequencies of transgenic fetuses and piglets. The transgenic animals showed normal development and persistent reporter gene expression for >12 months. Molecular hallmarks of transposition were confirmed by analysis of 25 genomic insertion sites. We demonstrate germ-line transmission, segregation of individual transposons, and continued, copy number-dependent transgene expression in F1-offspring. In addition, we demonstrate target-selected gene insertion into transposon-tagged genomic loci by Cre-loxP-based cassette exchange in somatic cells followed by nuclear transfer. Transposase-catalyzed transgenesis in a large mammalian species expands the arsenal of transgenic technologies for use in domestic animals and will facilitate the development of large animal models for human diseases

A Critical Systems Approach to Social Learning: Building Adaptive Capacity in Social, Ecological, Epistemological (SEE) Systems

This paper presents a conceptual tool, or heuristic, for describing the epistemological context for social learning within complex social-ecological systems. The heuristic integrates several definitions of social learning that emphasize the importance of critical reflection and its collaborative nature and that it is rooted in and oriented toward practice through social interactions. The conceptual tool is useful in identifying and conceptually mapping different perspectives based on types of learning described along three dimensions: typology of knowledge; different levels of critical reflection; and scale. The heuristic was originally developed in the context of an environmental planning process in southern Ontario, Canada, and is applied to identifying barriers and bridges to social learning in the case of flood damage reduction in a remote First Nations community in northern Ontario, Canada

Finding Common Ground: A Critical Review of Land Use and Resource Management Policies in Ontario, Canada and their Intersection with First Nations

This article provides an in-depth analysis of selective land use and resource management policies in the Province of Ontario, Canada. It examines their relative capacity to recognize the rights of First Nations and Aboriginal peoples and their treaty rights, as well as their embodiment of past Crown–First Nations relationships. An analytical framework was developed to evaluate the manifest and latent content of 337 provincial texts, including 32 provincial acts, 269 regulatory documents, 16 policy statements, and 5 provincial plans. This comprehensive document analysis classified and assessed how current provincial policies address First Nation issues and identified common trends and areas of improvement. The authors conclude that there is an immediate need for guidance on how provincial authorities can improve policy to make relationship-building a priority to enhance and sustain relationships between First Nations and other jurisdictions

The Far North Act (2010) Consultative Process: A New Beginning or the Reinforcement of an Unacceptable Relationship in Northern Ontario, Canada?

In northern Ontario, Canada, there have been two “negotiated” documents that required consultation between First Nations and the federated government of the land: Treaty No. 9 signed in 1905-1906 (Dominion of Canada, with the concurrence of the Province of Ontario) and Ontario’s Far North Act (2010). Treaty No. 9 has defined the relationship between First Nations and Canada; while, the Far North Act will define the relationship with Ontario. This article evaluated whether the Far North Act marked a new beginning or the reinforcement of an unacceptable relationship, using primary and secondary data analyses. Analyses revealed that the passing of the Far North Act was not a new beginning, but the continuation of an unacceptable relationship

A First Nations-led social innovation: a moose, a gold mining company, and a policy window

A recent focusing event involving the death of a moose in the Dome open-pit mine in the community of Timmins, in northern Ontario, Canada has triggered the opening of a policy window related to the governance of resource extraction by indigenous peoples. This event, combined with several other factors, including: (1) the high price of gold; (2) a mining company seeking to demonstrate corporate social responsibility to repair its international reputation with indigenous peoples by supporting an innovative, local Indigenous-led initiative; and (3) a new policy context, including Supreme Court of Canada decisions and provisions in the updated Ontario Mining Act, that require meaningful consultation with indigenous peoples has led to the emergence of a indigenous peoples-led collaborative, social innovation. This policy window allowed for the formation of an unprecedented council of indigenous knowledge holders (elders) and traditional practitioners to help inform mine restoration and practice as well as to foster the resurgence of traditional language and culture in local indigenous communities. Here, we document this unprecedented social change opportunity