Model updating using uncertain experimental modal data

The propagation of parameter uncertainty in structural dynamics has become a feasible method to determine the probabilistic description of the vibration response of industrial scale �nite element models. Though methods for uncertainty propagation have been developed extensively, the quanti�cation of parameter uncertainty has been neglected in the past. But a correct assumption for the parameter variability is essential for the estimation of the uncertain vibration response. This paper shows how to identify model parameter means and covariance matrix from uncertain experimental modal test data. The common gradient based approach from deterministic computational model updating was extended by an equation that accounts for the stochastic part. In detail an inverse approach for the identi�cation of statistical parametric properties will be presented which will be applied on a numerical model of a replica of the GARTEUR SM-AG19 benchmark structure. The uncertain eigenfrequencies and mode shapes have been determined in an extensive experimental modal test campaign where the aircraft structure was tested repeatedly while it was 130 times dis- and reassembled in between each experimental modal analysis

Unraveling the senses of Phytophthora; leads to novel control strategies?

Oomycetes cause devastating diseases on plants and animals. They cause major yield losses in many crop plants and their control heavily depends on agrochemicals. This is certainly true for the potato late blight pathogen Phytophthora infestans. Strong concerns about adverse effects of agrochemicals on food safety and environment are incentives for the development of novel, environmental friendly control strategies preferably based on natural products. Cyclic lipopeptides (CLPs) were recently discovered as a new class of natural compounds with strong activities against oomycetes including Phytophthora. CLPs lyse zoospores, inhibit mycelial growth and effectively reduce late blight disease. In order to unravel how Phytophthora senses CLPs and other environmental signals we follow two approaches. On the one hand, we monitor genome wide changes in gene expression induced by CLPs with the aim to identify the cellular pathways targeted by CLPs. On the other hand, we analyse components of ubiquitous signal transduction pathways with the aim to identify features that are unique for Phytophthora or oomycetes and, hence, could be suitable targets for novel anti-oomycete agents. Mining and comparing whole genome sequences have revealed that Phytophthora harbours many novel phospholipid modifying enzymes, unique for oomycetes. They have aberrant combinations of catalytic and regulatory domains occasionally combined with transmembrane domains. The latter resemble receptors that might be activated by extracellular ligands. Phospholipids, the substrates of these enzymes, are structural membrane components that also function in signalling. Together these findings open new avenues of research aimed at target-discovery in oomycetes

Avirulentiegenen in Phytophthora infestans, de veroorzaker van de aardappelziekte

Een proefschrift van Wageningen Universiteit beschrijft het karteren en kloneren van Avr-genen in Phytophthora infestans. Ook is de fenotypische en genotypische diversiteit in P. infestans-veldisolaten in Noord China onderzoch

Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling

In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other nuclei within heterokaryotic cells of the mycelium. Here we report experiments exploring the mechanism of internuclear gene silencing in P. infestans. The DNA methylation inhibitor 5-azacytidine induced reversion of the inf1-silenced state. Also, the histone deacetylase inhibitor trichostatin-A was able to reverse inf1 silencing. inf1-expression levels returned to the silenced state when the inhibitors were removed except in non-transgenic inf1-silenced strains that were generated via internuclear gene silencing, where inf1 expression was restored permanently. Therefore, inf1-transgenic sequences are required to maintain the silenced state. Prolonged culture of non-transgenic inf1-silenced strains resulted in gradual reactivation of inf1 gene expression. Nuclease digestion of inf1-silenced and non-silenced nuclei showed that inf1 sequences in silenced nuclei were less rapidly degraded than non-silenced inf1 sequences. Bisulfite sequencing of the endogenous inf1 locus did not result in detection of any cytosine methylation. Our findings suggest that the inf1-silenced state is based on chromatin remodelling

Biologically active Phytophthora mating hormone prepared by catalytic asymmetric total synthesis

A Phytophthora mating hormone with an array of 1,5-stereogenic centers has been synthesized by using our recently developed methodology of catalytic enantioselective conjugate addition of Grignard reagents. We applied this methodology in a diastereo- and enantioselective iterative route and obtained two of the 16 possible stereoisomers of Phytophthora hormone α1. These synthetic stereoisomers induced the formation of sexual spores (oospores) in A2 mating type strains of three heterothallic Phytophthora species, P. infestans, P. capsici, and P. nicotianae but not in A1 mating type strains. The response was concentration-dependent, and the oospores were viable. These results demonstrate that the biological activity of the synthetic hormone resembles that of the natural hormone α1. Mating hormones are essential components in the sexual life cycle of a variety of organisms. For plant pathogens like Phytophthora, sexual reproduction is important as a source of genetic variation. Moreover, the thick-walled oospores are the most durable propagules that can survive harsh environmental conditions. Sexual reproduction can thus greatly affect disease epidemics. The availability of synthetic compounds mimicking the activity of Phytophthora mating hormone will be instrumental for further unravelling sexual reproduction in this important group of plant pathogens.

Dynamische ziekteverwekkers; wat we (willen) weten over oömyceten

Inaugurele rede bij de aanvaarding van het ambt van persoonlijk hoogleraar bij het Laboratorium voor Fytopathologie aan Wageningen Universiteit op 11 juni 2009: "Dynamische ziekteverwekkers : Wat we (willen) weten over oömyceten". In deze rede over oömyceten wordt ingegaan op de plaats van deze ziekteverwekker in de evolutie. Daarna op de oömoceet Phytophthora infestans, de verwekker van de aardappelziekte, ziektebestrijding en resistentieveredeling, de diversiteit van Phytophthora en zijn gastheren en de genetische blauwdruk: het DNA, de genen, de effectoren en de resistentiegene

Pan-European Soil Erosion Risk Assessment: The PESEA Map verson 1, October 2003

Abstract not availableJRC.H-Institute for environment and sustainability (Ispra