1,487 research outputs found
Genetic stability of pneumococcal isolates during 35 days of human experimental carriage
Background
Pneumococcal carriage is a reservoir for transmission and a precursor to pneumococcal disease. The experimental human pneumococcal carriage model provides a useful tool to aid vaccine licensure through the measurement of vaccine efficacy against carriage (VEcol). Documentation of the genetic stability of the experimental human pneumococcal carriage model is important to further strengthen confidence in its safety and conclusions, enabling it to further facilitate vaccine licensure through providing evidence of VEcol.
Methods
229 isolates were sequenced from 10 volunteers in whom experimental human pneumococcal carriage was established, sampled over a period of 35 days. Multiple isolates from within a single volunteer at a single time provided a deep resolution for detecting variation. HiSeq data from the isolates were mapped against a PacBio reference of the inoculum to call variable sites.
Results
The observed variation between experimental carriage isolates was minimal with the maximum SNP distance between any isolate and the reference being 3 SNPs.
Conclusion
The low-level variation described provides evidence for the stability of the experimental human pneumococcal carriage model over 35 days, which can be reliably and confidently used to measure VEcol and aid future progression of pneumococcal vaccination
Magnetic ordering of Mn sublattice, dense Kondo lattice behavior of Ce in (RPd3)8Mn (R = La, Ce)
We have synthesized two new interstitial compounds (RPd3)8Mn (R = La and Ce).
The Mn ions present in "dilute" concentration of just 3 molar percent form a
sublattice with an unusually large Mn-Mn near neighbor distance of ~ 85 nm.
While the existence of (RPd3)8M (where M is a p-block element) is already
documented in the literature, the present work reports for the first time the
formation of this phase with M being a 3d element. In (LaPd3)8Mn, the Mn
sub-lattice orders antiferromagnetically as inferred from the peaks in
low-field magnetization at 48 K and 23 K. The latter peak progressively shifts
towards lower temperatures in increasing magnetic field and disappears below
1.8 K in a field of ~ 8 kOe. On the other hand in (CePd3)8Mn the Mn sublattice
undergoes a ferromagnetic transition around 35 K. The Ce ions form a dense
Kondo-lattice and are in a paramagnetic state at least down to 1.5 K. A
strongly correlated electronic ground state arising from Kondo effect is
inferred from the large extrapolated value of C/T = 275 mJ/Ce-mol K^2 at T = 0
K. In contrast, the interstitial alloys RPd3Mnx (x = 0.03 and 0.06), also
synthesized for the first time, have a spin glass ground state due to the
random distribution of the Mn ions over the available "1b" sites in the parent
RPd3 crystal lattice.Comment: 18 figures and 20 pages of text documen
A comparison of braking and differential control of road vehicle yaw-sideslip dynamics
Two actuation mechanisms are considered for the comparison of performance capabilities
in improving the yaw–sideslip handling characteristics of a road vehicle. Yaw moments are generated
either by the use of single-wheel braking or via driveline torque distribution using an overdriven active
rear differential. For consistency, a fixed reference vehicle system is used, and the two controllers are
synthesized via a single design methodology. Performance measures relate to both open-loop and
closed-loop driving demands, and include both on-centre and limit handling manoeuvres
The embedding method beyond the single-channel case: Two-mode and Hubbard chains
We investigate the relationship between persistent currents in multi-channel
rings containing an embedded scatterer and the conductance through the same
scatterer attached to leads. The case of two uncoupled channels corresponds to
a Hubbard chain, for which the one-dimensional embedding method is readily
generalized. Various tests are carried out to validate this new procedure, and
the conductance of short one-dimensional Hubbard chains attached to perfect
leads is computed for different system sizes and interaction strengths. In the
case of two coupled channels the conductance can be obtained from a statistical
analysis of the persistent current or by reducing the multi-channel scattering
problem to several single-channel setups.Comment: 14 pages, 13 figures, submitted for publicatio
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Toward Common Data Elements for International Research in Long-term Care Homes: Advancing Person-Centered Care
To support person-centered, residential long-term care internationally, a consortium of researchers in medicine, nursing, behavioral, and social sciences from 21 geographically and economically diverse countries have launched the WE-THRIVE consortium to develop a common data infrastructure. WE-THRIVE aims to identify measurement domains that are internationally relevant, including in low-, middle-, and high-income countries, prioritize concepts to operationalize domains, and specify a set of data elements to measure concepts that can be used across studies for data sharing and comparisons. This article reports findings from consortium meetings at the 2016 meeting of the Gerontological Society of America and the 2017 meeting of the International Association of Gerontology and Geriatrics, to identify domains and prioritize concepts, following best practices to identify common data elements (CDEs) that were developed through the US National Institutes of Health/National Institute of Nursing Research's CDEs initiative. Four domains were identified, including organizational context, workforce and staffing, person-centered care, and care outcomes. Using a nominal group process, WE-THRIVE prioritized 21 concepts across the 4 domains. Several concepts showed similarity to existing measurement structures, whereas others differed. Conceptual similarity (convergence; eg, concepts in the care outcomes domain of functional level and harm-free care) provides further support of the critical foundational work in LTC measurement endorsed and implemented by regulatory bodies. Different concepts (divergence; eg, concepts in the person-centered care domain of knowing the person and what matters most to the person) highlights current gaps in measurement efforts and is consistent with WE-THRIVE's focus on supporting resilience and thriving for residents, family, and staff. In alignment with the World Health Organization's call for comparative measurement work for health systems change, WE-THRIVE's work to date highlights the benefits of engaging with diverse LTC researchers, including those in low-, middle-, and high-income countries, to develop a measurement infrastructure that integrates the aspirations of person-centered LTC
Suppression of inhomogeneous broadening in rf spectroscopy of optically trapped atoms
We present a novel method for reducing the inhomogeneous frequency broadening
in the hyperfine splitting of the ground state of optically trapped atoms. This
reduction is achieved by the addition of a weak light field, spatially
mode-matched with the trapping field and whose frequency is tuned in-between
the two hyperfine levels. We experimentally demonstrate the new scheme with Rb
85 atoms, and report a 50-fold narrowing of the rf spectrum
Fano Resonances in Electronic Transport through a Single Electron Transistor
We have observed asymmetric Fano resonances in the conductance of a single
electron transistor resulting from interference between a resonant and a
nonresonant path through the system. The resonant component shows all the
features typical of quantum dots, but the origin of the non-resonant path is
unclear. A unique feature of this experimental system, compared to others that
show Fano line shapes, is that changing the voltages on various gates allows
one to alter the interference between the two paths.Comment: 8 pages, 6 figures. Submitted to PR
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