The autoregulatory translational control element of poly(A)-binding protein mRNA forms a heteromeric ribonucleoprotein complex

Repression of poly(A)-binding protein (PABP) mRNA translation involves the binding of PABP to the adenine-rich autoregulatory sequence (ARS) in the 5′-untranslated region of its own mRNA. In this report, we show that the ARS forms a complex in vitro with PABP, and two additional polypeptides of 63 and 105 kDa. The 63 and 105 kDa polypeptides were identified, as IMP1, an ortholog of chicken zip-code binding polypeptide, and UNR, a PABP binding polypeptide, respectively, by mass spectrometry of the ARS RNA affinity purified samples. Using a modified ribonucleoprotein (RNP) immunoprecipitation procedure we further show that indeed, both IMP1 and UNR bind to the ARS containing reporter RNA in vivo. Although both IMP1 and UNR could bind independently to the ARS RNA in vitro, their RNA-binding ability was stimulated by PABP. Mutational analyses of the ARS show that the presence of four of the six oligo(A) regions of the ARS was sufficient to repress translation and the length of the conserved pyrimidine spacers between the oligo(A) sequences was important for ARS function. The ability of mutant ARS RNAs to form the PABP, IMP1 and UNR containing RNP complex correlates well with the translational repressor activity of the ARS. There is also a direct relationship between the length of the poly(A) RNAs and their ability to form a trimeric complex with PABP, and to repress mRNA translation. UV crosslinking studies suggest that the ARS is less efficient than a poly(A) RNA of similar length, to bind to PABP. We show here that the ARS cannot efficiently form a trimeric complex with PABP; therefore, additional interactions with IMP1 and UNR to form a heteromeric RNP complex may be required for maximal repression of PABP mRNA translation under physiological conditions

Global Management Effectiveness Study: Integrated Social and Ecological Report for Non-node and Node Sites

The purpose of this study is to provide a critical assessment of the implementation, impact, and performance of Marine Managed Area (MMA) projects to serve as a basis for improved planning and implementation of new MMA projects worldwide. The specific objectives of the study are (1) to determine the socioeconomic, governance and ecological effects of MMAs; (2) to determine the critical factors influencing MMA effects, as well as the impact of the timing of those factors on the effects of the MMA; and (3) to provide tools for predicting MMA effects based on ecological, socioeconomic and governance variable

Factors influencing the work performance of Mitanins (ASHA) in Bilaspur district, Chhattisgarh, India: a cross sectional study

Background: The Mitanin programme is a community health volunteer programme which was initiated by the Government of Chhattisgarh, India in 2002. The Mitanin programme further progressed to lay foundation for the Accredited Social Health Activists (ASHA) programme by the National Rural Health Mission. There is robust indication that the Community health workers could significantly contribute towards the betterment of health outcomes. The objective of the study was to study the factors influencing the work performance of Mitanins in Bilaspur district, Chhattisgarh, India.Methods: A community based cross sectional study was carried out among 180 Mitanins selected through multistage random sampling in Bilaspur district, Chhattisgarh, India during March to August 2015. A predesigned, pretested questionnaire was used for data collection.Results: Regarding counselling to pregnant mother on maternal and child health about (65.6%) Mitanins were having knowledge and out of these prevalence of its practice was found to be (75.4%). Their less knowledge for content of responsibility significantly affected their practices in community. Other major factors influencing their work performance are better training sessions (37.8%), support from health staff (65.6%), drug kit refilling (80%), and fewer hurdles in receiving incentives (85.6%.Conclusions: Less knowledge of the work component, less cooperation from health staff, delay refilling of drug kit, incentive oriented practices, delayed and inadequate payment of incentives for Mitanins influences the work performance

Spatial analysis of malaria incidence at the village level in areas with unstable transmission in Ethiopia

<p>Abstract</p> <p>Background</p> <p>Malaria is the leading cause of morbidity and mortality in Ethiopia, accounting for over five million cases and thousands of deaths annually. The risks of morbidity and mortality associated with malaria are characterized by spatial and temporal variation across the country. This study examines the spatial and temporal patterns of malaria transmission at the local level and implements a risk mapping tool to aid in monitoring and disease control activities.</p> <p>Methods</p> <p>In this study, we examine the global and local patterns of malaria distribution in 543 villages in East Shoa, central Ethiopia using individual-level morbidity data collected from six laboratory and treatment centers between September 2002 and August 2006.</p> <p>Results</p> <p>Statistical analysis of malaria incidence by sex, age, and village through time reveal the presence of significant spatio-temporal variations. Poisson regression analysis shows a decrease in malaria incidence with increasing age. A significant difference in the malaria incidence density ratio (IDRs) is detected in males but not in females. A significant decrease in the malaria IDRs with increasing age is captured by a quadratic model. Local spatial statistics reveals clustering or hot spots within a 5 and 10 km distance of most villages in the study area. In addition, there are temporal variations in malaria incidence.</p> <p>Conclusion</p> <p>Malaria incidence varies according to gender and age, with males age 5 and above showing a statistically higher incidence. Significant local clustering of malaria incidence occurs between pairs of villages within 1–10 km distance lags. Malaria incidence was higher in 2002–2003 than in other periods of observation. Malaria hot spots are displayed as risk maps that are useful for monitoring and spatial targeting of prevention and control measures against the disease.</p

Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020.

Severe acute respiratory syndrome coronavirus 2 viral load in the upper respiratory tract peaks around symptom onset and infectious virus persists for 10 days in mild-to-moderate coronavirus disease (n = 324 samples analysed). RT-PCR cycle threshold (Ct) values correlate strongly with cultivable virus. Probability of culturing virus declines to 8% in samples with Ct > 35 and to 6% 10 days after onset; it is similar in asymptomatic and symptomatic persons. Asymptomatic persons represent a source of transmissible virus

PbS-ZnO Solar Cell: A Numerical Simulation

Nanoscale PbS, especially quantum dots (QDs) are of interest in applications, such as, solar cells and photodetectors because of tunability of band gap from 0.5 to 3 eV. Recently, ZnO/PbS solar cells with 8.55 % conversion efficiency have been reported with films made deposited from ligand exchanged PbS QDs. However, nanocrystalline PbS is easier to fabricate than QDs. This paper reports theoretical investigation into the use of nanocrystalline PbS in place of QDs as solar cell absorber. Solar cells with a structure of SLG/ITO/ZnO or CdS/PbS/Al was simulated using SCAPS software. We have used two n-type materials one is ZnO and second is CdS. The comparative simulated device performance was studied by currentvoltage (I-V) characteristics and quantum efficiency (QE). The final results reveal a power conversion efficiency of 18.5 % for solar cells with p-PbS as absorber and n-ZnO as buffer and 16.8 % for n-CdS buffer layer

Real-Time Mobile Teleophthalmology for the Detection of Eye Disease in Minorities and Low Socioeconomics At-Risk Populations

Purpose: To examine the benefits and feasibility of a mobile, real-time, community-based, teleophthalmology program for detecting eye diseases in the New York metro area. Design: Single site, nonrandomized, cross-sectional, teleophthalmologic study. Methods: Participants underwent a comprehensive evaluation in a Wi-Fi-equipped teleophthalmology mobile unit. The evaluation consisted of a basic anamnesis with a questionnaire form, brief systemic evaluations and an ophthalmologic evaluation that included visual field, intraocular pressure, pachymetry, anterior segment optical coherence tomography, posterior segment optical coherence tomography, and nonmydriatic fundus photography. The results were evaluated in real-time and follow-up calls were scheduled to complete a secondary questionnaire form. Risk factors were calculated for different types of ophthalmological referrals. Results: A total of 957 participants were screened. Out of 458 (48%) participants that have been referred, 305 (32%) had glaucoma, 136 (14%) had narrow-angle, 124 (13%) had cataract, 29 had (3%) diabetic retinopathy, 9 (1%) had macular degeneration, and 97 (10%) had other eye disease findings. Significant risk factors for ophthalmological referral consisted of older age, history of high blood pressure, diabetes mellitus, Hemoglobin A1c measurement of ≥6.5, and stage 2 hypertension. As for the ocular parameters, all but central corneal thickness were found to be significant, including having an intraocular pressure \u3e21 mm Hg, vertical cup-to-disc ratio ≥0.5, visual field abnormalities, and retinal nerve fiber layer thinning. Conclusions: Mobile, real-time teleophthalmology is both workable and effective in increasing access to care and identifying the most common causes of blindness and their risk factors