Pierre Hadot: el cuidado de sí y la mayéutica socrática como ejercicio espiritual

Se presenta otro rostro del conceptode la mayéutica de Sócrates, toda vezque aquí no se acentúa su acepción epistemológica,sino su connotación en tanto ejercicioespiritual. Se parte de las consideraciones queha realizado el estudioso de origen francés,Pierre Hadot, quien se ha preocupado por eltema de los ejercicios espirituales en la filosofíaantigua. Conectamos, pues, la noción demayéutica socrática, en tanto ejercicio espiritualcon la noción de cuidado de sí. Consideramosque esta manera de interpretar el pensamientodel antiguo Sócrates representa un aporteimportante para reavivar y continuar el diálogofilosófico con los antiguos.Se presenta otro rostro del conceptode la mayéutica de Sócrates, toda vezque aquí no se acentúa su acepción epistemológica,sino su connotación en tanto ejercicioespiritual. Se parte de las consideraciones queha realizado el estudioso de origen francés,Pierre Hadot, quien se ha preocupado por eltema de los ejercicios espirituales en la filosofíaantigua. Conectamos, pues, la noción demayéutica socrática, en tanto ejercicio espiritualcon la noción de cuidado de sí. Consideramosque esta manera de interpretar el pensamientodel antiguo Sócrates representa un aporteimportante para reavivar y continuar el diálogofilosófico con los antiguos

Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids

The study of fallopian tube (FT) function in health and disease has been hampered by limited knowledge of FT stem cells and lack of in vitro models of stem cell renewal and differentiation. Using optimized organoid culture conditions to address these limitations, we find that FT stem cell renewal is highly dependent on WNT/β-catenin signaling and engineer endogenous WNT/β-catenin signaling reporter organoids to biomark, isolate, and characterize these cells. Using functional approaches, as well as bulk and single-cell transcriptomics analyses, we show that an endogenous hormonally regulated WNT7A-FZD5 signaling axis is critical for stem cell renewal and that WNT/β-catenin pathway-activated cells form a distinct transcriptomic cluster of FT cells enriched in extracellular matrix (ECM) remodeling and integrin signaling pathways. Overall, we provide a deep characterization of FT stem cells and their molecular requirements for self-renewal, paving the way for mechanistic work investigating the role of stem cells in FT health and disease

Estudio de los clientes segmento personal plus de Bancolombia frente al uso de transacciones electrónicas en Cúcuta

61 Páginas.El crecimiento de los servicios bancarios por medios electrónicos ha tenido un importante crecimiento, impulsado principalmente por el aumento en la cobertura de computadores y el acceso a Internet. Aún así, se siguen presentando dificultades para que las personas accedan con la confianza y seguridad esperada al portafolio que se ofrece de forma virtual. El presente estudio se centra en estas barreras de uso de los servicios online para el pago y transacciones de usuarios de la banca. Para esto, el presente proyecto tiene como objetivo general estudiar los clientes del segmento personal plus de Bancolombia Cúcuta, frente al uso de las transacciones electrónicas en el primer semestre de 2013

Weighing Risk Factors for Female Victimization in the Context of Romantic Relationship Initiation

Research has paid little attention to the link between the characteristics of the relational context where adolescents are likely to initiate their romantic relationships and teen dating violence (TDV). Hence, the findings are still scattered. This study examined different risks in the female teenagers’ relational context (peer group characteristics, participants’ risky activities, and pressure to start dating) and their TDV victimization, which had not been previously studied in the Spanish population. The moderating role of parental monitoring strategies was also analyzed. Participants were 1248 Spanish female teenagers who completed measures of the aforementioned factors. Highly victimized girls reported having more deviant and older male peers, receiving more pressure to start dating, and using more alcohol and drugs than participants with low victimization did. High parental monitoring was only effective to prevent TDV victimization in low-risk relational contexts. The findings extend prior research by providing evidence of the risk of pressure to start dating and low effectiveness of parental monitoring against high-risk peers. They also highlight the need to reduce specific risks for TDV in the adolescent relational context

The Repertoire of Serous Ovarian Cancer Non-genetic Heterogeneity Revealed by Single-Cell Sequencing of Normal Fallopian Tube Epithelial Cells

The inter-differentiation between cell states promotes cancer cell survival under stress and fosters non-genetic heterogeneity (NGH). NGH is, therefore, a surrogate of tumor resilience but its quantification is confounded by genetic heterogeneity. Here we show that NGH in serous ovarian cancer (SOC) can be accurately measured when informed by the molecular signatures of the normal fallopian tube epithelium (FTE) cells, the cells of origin of SOC. Surveying the transcriptomes of ∼6,000 FTE cells, predominantly from non-ovarian cancer patients, identified 6 FTE subtypes. We used subtype signatures to deconvolute SOC expression data and found substantial intra-tumor NGH. Importantly, NGH-based stratification of ∼1,700 tumors robustly correlated with survival. Our findings lay the foundation for accurate prognostic and therapeutic stratification of SOC. Using single-cell RNA sequencing, Hu et al. identify six subtypes of fallopian tube epithelium (FTE) cells in normal human fallopian tube tissues. The FTE cellular subtypes reveal intra-tumoral heterogeneity in serous ovarian cancer (SOC) and define SOC subtypes that correlate with patient prognosis.</p

Development of a Panel of Genome-Wide Ancestry Informative Markers to Study Admixture Throughout the Americas

Most individuals throughout the Americas are admixed descendants of Native American, European, and African ancestors. Complex historical factors have resulted in varying proportions of ancestral contributions between individuals within and among ethnic groups. We developed a panel of 446 ancestry informative markers (AIMs) optimized to estimate ancestral proportions in individuals and populations throughout Latin America. We used genome-wide data from 953 individuals from diverse African, European, and Native American populations to select AIMs optimized for each of the three main continental populations that form the basis of modern Latin American populations. We selected markers on the basis of locus-specific branch length to be informative, well distributed throughout the genome, capable of being genotyped on widely available commercial platforms, and applicable throughout the Americas by minimizing within-continent heterogeneity. We then validated the panel in samples from four admixed populations by comparing ancestry estimates based on the AIMs panel to estimates based on genome-wide association study (GWAS) data. The panel provided balanced discriminatory power among the three ancestral populations and accurate estimates of individual ancestry proportions (R2>0.9 for ancestral components with significant between-subject variance). Finally, we genotyped samples from 18 populations from Latin America using the AIMs panel and estimated variability in ancestry within and between these populations. This panel and its reference genotype information will be useful resources to explore population history of admixture in Latin America and to correct for the potential effects of population stratification in admixed samples in the region

Promises and challenges of adoptive T-cell therapies for solid tumours

From Springer Nature via Jisc Publications RouterHistory: received 2020-11-09, rev-recd 2021-02-22, accepted 2021-03-04, registration 2021-03-04, pub-electronic 2021-03-29, online 2021-03-29, pub-print 2021-05-25Publication status: PublishedFunder: DH | National Institute for Health Research (NIHR); doi: https://doi.org/10.13039/501100000272; Grant(s): RCF18/046Funder: Ovarian Cancer Action; doi: https://doi.org/10.13039/501100000299; Grant(s): HER000762Abstract: Cancer is a leading cause of death worldwide and, despite new targeted therapies and immunotherapies, many patients with advanced-stage- or high-risk cancers still die, owing to metastatic disease. Adoptive T-cell therapy, involving the autologous or allogeneic transplant of tumour-infiltrating lymphocytes or genetically modified T cells expressing novel T-cell receptors or chimeric antigen receptors, has shown promise in the treatment of cancer patients, leading to durable responses and, in some cases, cure. Technological advances in genomics, computational biology, immunology and cell manufacturing have brought the aspiration of individualised therapies for cancer patients closer to reality. This new era of cell-based individualised therapeutics challenges the traditional standards of therapeutic interventions and provides opportunities for a paradigm shift in our approach to cancer therapy. Invited speakers at a 2020 symposium discussed three areas—cancer genomics, cancer immunology and cell-therapy manufacturing—that are essential to the effective translation of T-cell therapies in the treatment of solid malignancies. Key advances have been made in understanding genetic intratumour heterogeneity, and strategies to accurately identify neoantigens, overcome T-cell exhaustion and circumvent tumour immunosuppression after cell-therapy infusion are being developed. Advances are being made in cell-manufacturing approaches that have the potential to establish cell-therapies as credible therapeutic options. T-cell therapies face many challenges but hold great promise for improving clinical outcomes for patients with solid tumours