Teacher training in logo programming by using preconstructed reusable microworlds

This paper suggests an alternative training approach and relative training material for introductory courses to Logo programming by using a series of activities and preconstructed reusable microworlds developed in the multimedia programming environment MicroWorlds Pro. The proposed approach and material aims to scaffold the gradual familiarization of the trainees with Logo and the programming environment by applying in action the constructionist reasoning. The training material is characterized by a gradual increase in complexity and difficulty and exploits the gradually acquired experience of the trainees by providing them with tools that they are in position to use. In this paper the design principles of the training material, the activities and the preconstructed microworlds are described and some key points of implementation, pilot and experimental study are reported. Finally, conclusions are given and special issues of interest and future research plans are discussed. © Common Ground, Katerina Glezou, Maria Grigoriadou

Educational online social networking in Greece: A case study of a Greek educational online social network

Over the past few years, the rate of Web 2.0 diffusion has been dramatic. Social media and emerging technologies such as wikis, blogging, online gaming, tagging and social networking have spread widely and rapidly and are gaining increased attention for use in education. Finding coherence in the midst of rapid changes is very difficult. This study attempts to contribute to an on-going dialogue about the trends and implications of online social networking in education and especially in lifelong learning. The paper provides an overview of the current status of educational online social networking in Greece and presents a case study of the Greek Educational Online Social Network (EOSN) "Logo in Education: a learning community of practice" (http://logogreekworld.ning.com/). The specific EOSN has been created in late May 2009 by an independent initiative of an educator with the purpose to facilitate communications and interactions, exchange of information, ideas and educational material and to promote co-operation and collaboration between the members of the educational community mostly interested in Logo programming language and philosophy. In this paper we describe main features of the EOSN; discuss key aspects, general implementation details, changes and developments and conclude with considerations for future work. © Common Ground, Katerina Glezou, Maria Grigoriadou, Maria Samarakou

MicroRNA-155-5p Overexpression in Peripheral Blood Mononuclear Cells of Chronic Lymphocytic Leukemia Patients Is a Novel, Independent Molecular Biomarker of Poor Prognosis

MicroRNA-155-5p (miR-155-5p) is a proinflammatory, oncogenic miRNA, involved in various physiological processes, including hematopoiesis, immunity, inflammation, and cell lineage differentiation. It regulates important transcription factors, such as E2F2, hypoxia-inducible factor 1 (HIF1), and FOXO3. Recently, the dysregulation of miR-155-5p expression has been linked to chronic lymphocytic leukemia (CLL) pathogenesis. In this research study, we investigated the potential diagnostic and prognostic value of miR-155-5p in CLL. To achieve our goal, we isolated total RNA from peripheral blood mononuclear cells (PBMCs) collected from 88 CLL patients and 36 nonleukemic blood donors and performed polyadenylation of total RNA and reverse transcription. Next, we quantified miR-155-5p levels using an in-house-developed real-time quantitative PCR method, before proceeding to extensive biostatistical analysis. Thus, it appears that miR-155-5p is significantly overexpressed in PBMCs of CLL patients and can distinguish them from nonleukemic population. Kaplan-Meier OS analysis and bootstrap univariate Cox regression showed that high miR-155-5p expression predicts inferior OS for CLL patients (p<0.001). Interestingly, miR-155-5p overexpression retains its unfavorable prognostic role in CLL patients stratified according to established prognostic factors [CD38 expression and mutational status of the immunoglobulin heavy chain variable region (IGHV)]. Thus, miR-155-5p appears as a promising, independent molecular biomarker of unfavorable prognosis in CLL

mRNA overexpression of the hypoxia inducible factor 1 alpha subunit gene (HIF1A): An independent predictor of poor overall survival in chronic lymphocytic leukemia

The hypoxia inducible factor 1 (HIF1) is a heterodimeric transcription factor that ultimately regulates cellular responses to changes in oxygen tension. In this study, we examined the potential diagnostic and prognostic potential of the mRNA expression of HIF1 regulatory α-subunit (HIF1A) in chronic lymphocytic leukemia (CLL). For this purpose, total RNA was isolated from peripheral blood mononuclear cells collected from 88 CLL patients and 33 non-leukemic blood donors, and poly(A)-RNA was reversely transcribed. HIF1A mRNA levels were quantified using real-time PCR. Kaplan-Meier survival analysis showed that high HIF1A mRNA expression predicts inferior overall survival for CLL patients (p = 0.001). Bootstrap univariate Cox regression analysis confirmed that HIF1A mRNA overexpression is a significant unfavorable prognosticator in CLL (hazard ratio = 3.75, bias-corrected and accelerated 95% confidence interval = 1.43–24.36, bootstrap p &lt; 0.001), independent of other established prognostic factors, including CD38 expression, the mutational status of the immunoglobulin heavy chain variable region (IGHV), and the clinical stage (Binet or Rai stage) or risk group (p &lt; 0.001 in all cases). Interestingly, HIF1A mRNA positivity retains its unfavorable prognostic value in distinct subgroups of patients, stratified according to established prognostic factors. Thus, HIF1A mRNA overexpression can be regarded as a promising, independent molecular biomarker of unfavorable prognosis in CLL. © 2016 Elsevier Lt

Combination therapy as a potential risk factor for the development of type 2 diabetes in patients with schizophrenia: The GOMAP study

Background: Schizophrenia (SCZ) is associated with increased risk of type 2 diabetes (T2D). The potential diabetogenic effect of concomitant application of psychotropic treatment classes in patients with SCZ has not yet been evaluated. The overarching goal of the Genetic Overlap between Metabolic and Psychiatric disease (GOMAP) study is to assess the effect of pharmacological, anthropometric, lifestyle and clinical measurements, helping elucidate the mechanisms underlying the aetiology of T2D. Methods: The GOMAP case-control study (Genetic Overlap between Metabolic and Psychiatric disease) includes hospitalized patients with SCZ, some of whom have T2D. We enrolled 1653 patients with SCZ; 611 with T2D and 1042 patients without T2D. This is the first study of SCZ and T2D comorbidity at this scale in the Greek population. We retrieved detailed information on first- and second-generation antipsychotics (FGA, SGA), antidepressants and mood stabilizers, applied as monotherapy, 2-drug combination, or as 3- or more drug combination. We assessed the effects of psychotropic medication, body mass index, duration of schizophrenia, number of hospitalizations and physical activity on risk of T2D. Using logistic regression, we calculated crude and adjusted odds ratios (OR) to identify associations between demographic factors and the psychiatric medications. Results: Patients with SCZ on a combination of at least three different classes of psychiatric drugs had a higher risk of T2D [OR 1.81 (95% CI 1.22-2.69); p=0.003] compared to FGA alone therapy, after adjustment for age, BMI, sex, duration of SCZ and number of hospitalizations. We did not find evidence for an association of SGA use or the combination of drugs belonging to two different classes of psychiatric medications with increased risk of T2D [1.27 (0.84-1.93), p=0.259 and 0.98 (0.71-1.35), p=0.885, respectively] compared to FGA use. Conclusions: We find an increased risk of T2D in patients with SCZ who take a combination of at least three different psychotropic medication classes compared to patients whose medication consists only of one or two classes of drugs. © 2018 The Author(s)

Upregulated hypoxia inducible factor 1α signaling pathway in high risk myelodysplastic syndrome and acute myeloid leukemia patients is associated with better response to 5-azacytidine—data from the Hellenic myelodysplastic syndrome study group

5-azacytidine (5-AZA) is considered the standard of care for patients with high-risk myelodysplastic syndromes (MDS) and patients with acute myeloid leukemia (AML) not candidate for intensive chemotherapy. However, even after an initial favorable response, almost all patients relapse, with the exact mechanisms underlying primary or secondary 5-AZA resistance remaining largely unknown. Several reports have previously demonstrated the significance of hypoxia in the regulation of both physiological and malignant hematopoiesis. In MDS, high hypoxia inducible factor 1α (Hif-1α) expression has been correlated with poor overall survival and disease progression, while its involvement in the disease&apos;s pathogenesis was recently reported. We herein investigated the possible association of the Hif-1α signaling pathway with response to 5-AZA therapy in MDS/AML patients. Our data demonstrated that 5-AZA-responders present with higher Hif-1α mRNA and protein expression compared to 5-AZA-non-responders/stable disease patients, before the initiation of therapy, while, interestingly, no significant differences in Hif-1α mRNA expression at the 6-month follow-up were observed. Moreover, we found that 5-AZA-responders exhibited elevated mRNA levels of the Hif-1α downstream targets lactate dehydrogenase a (LDHa) and BCL2 interacting protein 3 like (BNIP3L), a further indication of an overactivated Hif-1a signaling pathway in these patients. Kaplan–Meier survival analysis revealed a significant correlation between high Hif-1α mRNA expression and better survival rates, while logistic regression analysis showed that Hif-1α mRNA expression is an independent predictor of response to 5-AZA therapy. From the clinical point of view, apart from proposing Hif-1α mRNA expression as a significant predictive factor for response to 5-AZA, our data offer new perspectives on MDS combinational therapies, suggesting a potential synergistic activity of 5-AZA and Hif-1α inducers, such as propyl hydroxylases inhibitors (PHDi). © 2020 John Wiley &amp; Sons Ltd

Quantitative and qualitative analysis of regulatory T cells in B cell chronic lymphocytic leukemia

Accumulated data indicate a significant role of T cell dysfunction in the pathogenesis of chronic lymphocytic leukemia. In CLL, regulatory T cells are significantly higher and show lower apoptotic levels compared to healthy donors. We demonstrate that CLL derived CD4+CD25−CD127− and CD4+CD25lowCD127− subpopulations share a common immunophenotypic profile with conventional Tregs and are associated with advanced stage disease. We further provide evidence that the increased number of Tregs contributes indirectly to the proliferation of the CLL clone, by suppressing the proliferation of Teffs which in turn suppress CLL cells. These data are further supported by our observations that CLL derived Tregs appear rather incapable of inducing apoptosis of both normal B cells and CLL cells, in contrast to normal Tregs, suggesting an immunoediting effect of CLL cells on Tregs which negatively affects the functionality of the latter and contributes to the failure of Tregs in CLL to efficiently eliminate the abnormal clone. © 2017 Elsevier Lt

Bringing Research into the Classroom – The Citizen Science approach in schools.

The way science is approached in the classroom can be instrumental in dispelling negative stereotypes about science and scientific research in future generations. The present report looks at the Citizen Science approach as an opportunity to connect schools with the world of research to foster a better command of scientific processes in the young, raise their awareness of current issues faced in certain sectors and geographical regions, and help them make sense of the surrounding world. The purpose of this report is to provide a baseline for understanding the key conditions of successfully implementing citizen science activities in schools. This report is based on three main sources of information: (1) review of recent literature on Citizen Science and its applications in schools; (2) a collection of citizen science case studies selected by educational organisations in four countries (Belgium, Greece, Poland and Spain) and from the Scientix repository of resources; and (3) the discussions between project managers, project representatives and science educators participating in the 14th Science Projects Networking Event (SPNE14), organisedbyScientixwiththecollaborationoffour other organisations and projects – GFOSS, Jet Propulsion Theatre, EDU-ARCTIC and ERIS. The report includes three main sections. The first explores the current literature on citizen science, guided by three main questions: (1) how do we define citizen science, (2) what are the main actors involved in citizen science projects and how do they contribute, and (3) what are the particularities of citizen science activities run in the context of formal education. The second section illustrates 20 citizen science projects, indicating the target audiences and main areas of research covered, as well as a description of the activities, outlining which part of the scientific method is carried out by volunteers, and, when such information is available, descriptions of the roles and interactions between the researchers and citizen scientists. Finally, the third section presents a discussion on the case studies included in the report, with a focus on the main challenges and opportunities of bringing citizen science in schools. The main challenge of running citizen science activities in school which transpired from the literature review and the review of the case studies is the issue of balancing research and educational outcomes. For educational outcomes to be achieved, citizen science projects in schools need to go beyond engaging pupils only in data collection and simple analysis, and look to involving them in meaningful research practices, which will give them the opportunity to develop scientific inquiry skills. On the same line, if genuine science outcomes are to be met, the ‘novice’ scientists (teachers and their students) need to interact with the researchers in order to be exposed to the requirements of the scientific method, and be supported in implementing it. Research suggests that carefully designed projects, created in dialogue between schools (teachers) and researchers which take into consideration the needs and constraints of both groups can successfully achieve both goals.status: Published onlin