Buenas Prácticas Fitosanitarias

Buenas Prácticas FitosanitariasFil: Bulacio, Liliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argenti

Identifying the primary types of accidents that occur to horticultural workers in Argentina

Existen antecedentes sobre el uso incorrecto de agroquímicos y las precarias condiciones de vida de los horticultores argentinos. El objetivo de este estudio fue identificar los principales tipos de accidentes en ese grupo de trabajadores. Se tomó como zona testigo el cinturón hortícola del Gran La Plata. A través de encuestas a productores y entrevistas con informantes calificados se valoró: tipos de accidentes, situaciones en que se producen, zonas del cuerpo afectadas y gravedad de las lesiones. Se indagó sobre quién recomienda los tratamientos con agroquímicos y la disponibilidad de elementos de protección personal (EPP). De las encuestas realizadas surge que 52 % de los accidentes se produjeron por intoxicaciones por el uso de agroquímicos. La mayoría de las zonas del cuerpo se vieron afectadas. En 19 % de los casos se requirió internación. En el uso de agroquímicos, 35 % de los encuestados tienen asesoramiento técnico y el resto consulta principalmente a los vendedores. Sólo 20 % tenía los EPP completos, y 35% indicó que no los utiliza. Existe coincidencia entre los informantes calificados de la mayor probabilidad de accidentes durante la aplicación de agroquímicos, la realización de labores manuales y el uso del tractor.There are precedents regarding the incorrect use of agrichemicals and the precarious life conditions of Argentine horticultural workers. The objective of this study was to identify the primary types of accidents in this group of workers. The horticultural belt in Gran La Plata was used as witness area. Through surveys carried out with producers and interviews done with qualified informants, the following was evaluated: types of accidents, situations where they occur, body areas affected, and seriousness of the lesions. We investigated who recommends the treatments with agrichemicals, and the availability of personal protection elements (PPE). From the surveys carried out, data show that 52 % of the accidents were produced by intoxication from the use of agrichemicals. Most areas of the body were affected. In 19 % of the cases, hospitalization was necessary. In the use of agrichemicals, 35 % of those polled received technical advice and the rest consulted mainly the sellers. Only 20 % had complete PPE, and 35 % indicated they don't use them. There is an agreement among qualified informants regarding the greater probability of accidents while applying agrichemicals, performing manual labor and using the tractor.EEA San PedroFil: Paunero, Ignacio Eugenio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFerratto, Jorge. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Giuliani, Susana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Bulacio, Liliana. Universidad de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Panelo, Marta. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Amoia, Rita Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural Gran Buenos Aires; ArgentinaFil: Strassera, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural Gran Buenos Aires; ArgentinaFil: Granitto, Georgina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; La PlataFil: Pino, Mariana del. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; La PlataFil: Martinez, Susana. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Fortunato, Nicolás. Gobierno de la Provincia de Buenos Aires. Organismo Provincial para el Desarrollo Sostenible; ArgentinaFil: Fortunato, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Cambio Rural; ArgentinaFil: Tangorra, Mariana. Gobierno de la Provincia de Buenos Aires. Organismo Provincial para el Desarrollo Sostenible; ArgentinaFil: Andreau, Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Garbi, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales, ArgentinaFil: Martinez Quintana, Oscar Régulo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Area Metropolitana de Buenos Aires. Agencia de Extensión Rural Gran Buenos Aires; Argentin

Expansion of Adult Human Pancreatic Tissue Yields Organoids Harboring Progenitor Cells with Endocrine Differentiation Potential.

Generating an unlimited source of human insulin-producing cells is a prerequisite to advance β cell replacement therapy for diabetes. Here, we describe a 3D culture system that supports the expansion of adult human pancreatic tissue and the generation of a cell subpopulation with progenitor characteristics. These cells display high aldehyde dehydrogenase activity (ALDHhi), express pancreatic progenitors markers (PDX1, PTF1A, CPA1, and MYC), and can form new organoids in contrast to ALDHlo cells. Interestingly, gene expression profiling revealed that ALDHhi cells are closer to human fetal pancreatic tissue compared with adult pancreatic tissue. Endocrine lineage markers were detected upon in vitro differentiation. Engrafted organoids differentiated toward insulin-positive (INS+) cells, and circulating human C-peptide was detected upon glucose challenge 1 month after transplantation. Engrafted ALDHhi cells formed INS+ cells. We conclude that adult human pancreatic tissue has potential for expansion into 3D structures harboring progenitor cells with endocrine differentiation potential

Infected pancreatic necrosis: outcomes and clinical predictors of mortality. A post hoc analysis of the MANCTRA-1 international study

: The identification of high-risk patients in the early stages of infected pancreatic necrosis (IPN) is critical, because it could help the clinicians to adopt more effective management strategies. We conducted a post hoc analysis of the MANCTRA-1 international study to assess the association between clinical risk factors and mortality among adult patients with IPN. Univariable and multivariable logistic regression models were used to identify prognostic factors of mortality. We identified 247 consecutive patients with IPN hospitalised between January 2019 and December 2020. History of uncontrolled arterial hypertension (p = 0.032; 95% CI 1.135-15.882; aOR 4.245), qSOFA (p = 0.005; 95% CI 1.359-5.879; aOR 2.828), renal failure (p = 0.022; 95% CI 1.138-5.442; aOR 2.489), and haemodynamic failure (p = 0.018; 95% CI 1.184-5.978; aOR 2.661), were identified as independent predictors of mortality in IPN patients. Cholangitis (p = 0.003; 95% CI 1.598-9.930; aOR 3.983), abdominal compartment syndrome (p = 0.032; 95% CI 1.090-6.967; aOR 2.735), and gastrointestinal/intra-abdominal bleeding (p = 0.009; 95% CI 1.286-5.712; aOR 2.710) were independently associated with the risk of mortality. Upfront open surgical necrosectomy was strongly associated with the risk of mortality (p < 0.001; 95% CI 1.912-7.442; aOR 3.772), whereas endoscopic drainage of pancreatic necrosis (p = 0.018; 95% CI 0.138-0.834; aOR 0.339) and enteral nutrition (p = 0.003; 95% CI 0.143-0.716; aOR 0.320) were found as protective factors. Organ failure, acute cholangitis, and upfront open surgical necrosectomy were the most significant predictors of mortality. Our study confirmed that, even in a subgroup of particularly ill patients such as those with IPN, upfront open surgery should be avoided as much as possible. Study protocol registered in ClinicalTrials.Gov (I.D. Number NCT04747990)

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements