A Membrane Fusion Protein αSNAP Is a Novel Regulator of Epithelial Apical Junctions

Tight junctions (TJs) and adherens junctions (AJs) are key determinants of the structure and permeability of epithelial barriers. Although exocytic delivery to the cell surface is crucial for junctional assembly, little is known about the mechanisms controlling TJ and AJ exocytosis. This study was aimed at investigating whether a key mediator of exocytosis, soluble N-ethylmaleimide sensitive factor (NSF) attachment protein alpha (αSNAP), regulates epithelial junctions. αSNAP was enriched at apical junctions in SK-CO15 and T84 colonic epithelial cells and in normal human intestinal mucosa. siRNA-mediated knockdown of αSNAP inhibited AJ/TJ assembly and establishment of the paracellular barrier in SK-CO15 cells, which was accompanied by a significant down-regulation of p120-catenin and E-cadherin expression. A selective depletion of p120 catenin effectively disrupted AJ and TJ structure and compromised the epithelial barrier. However, overexpression of p120 catenin did not rescue the defects of junctional structure and permeability caused by αSNAP knockdown thereby suggesting the involvement of additional mechanisms. Such mechanisms did not depend on NSF functions or induction of cell death, but were associated with disruption of the Golgi complex and down-regulation of a Golgi-associated guanidine nucleotide exchange factor, GBF1. These findings suggest novel roles for αSNAP in promoting the formation of epithelial AJs and TJs by controlling Golgi-dependent expression and trafficking of junctional proteins

Creating and curating an archive: Bury St Edmunds and its Anglo-Saxon past

This contribution explores the mechanisms by which the Benedictine foundation of Bury St Edmunds sought to legitimise and preserve their spurious pre-Conquest privileges and holdings throughout the Middle Ages. The archive is extraordinary in terms of the large number of surviving registers and cartularies which contain copies of Anglo-Saxon charters, many of which are wholly or partly in Old English. The essay charts the changing use to which these ancient documents were put in response to threats to the foundation's continued enjoyment of its liberties. The focus throughout the essay is to demonstrate how pragmatic considerations at every stage affects the development of the archive and the ways in which these linguistically challenging texts were presented, re-presented, and represented during the Abbey’s history

The Transmission of two Secular Latin Songs

In the 15th and 16th centuries secular medieval Latin songs attracted the attention of musicians. Two examples (Vinum bonum et suave, and Potatores exquisiti), in their medieval and renaissance manifestations, highlight the differences of approach to music in the two period. Both are drinking songs but have associations with sacred repertoire; and seem to be connected to monastic culture; and the two seem to have migrated to disparate regions of Europe

Conductus as Analgesic

One of the difficulties in creating an adequate picture of the contextual situation for music, other than that clearly associated with the liturgy, in the Middle Ages, is the paucity of accounts describing performance circumstances. We know little about the social milieu and purposes attending genres marginal to the liturgy such as the conductus and thirteenth-century motet. A manuscript which seems to redress this problem, albeit for one very specific instance, is Vat. lat. 2854 in the Vatican library in Rome.This manuscript is unusual in that it contains not only music but a detailed account of why the music was written. The author, Bonaiutus de Casentino, active in the circle of Pope Boniface VIII, prepared the manuscript in the last decade of the thirteenth century at Rome. The document includes various poems, sacred and secular, as well as two Latin songs written in late Franconian notation. One of the pieces is a two-voice conductus (Hec medela corporalis) which was written, according to the account of Bonaiutus himself, in order to cure the maladies of an ailing pontif. The pontifical complaints seemed to be both psychological and intestinal in nature. It was the hope of Bonaiutus not only to provoke laughter (always a curative), but also to cleanse the papal bowels through his composition. Although one cannot generalize on the basis of this single incident, it does yield a fascinating glimpse into a possible venue for the conductus