Sleep Quality: A Mediator in the Pathway of Stress and Cold Symptom Severity

Stress is a known contributor to immune system suppression associated with higher illness susceptibility, including acute infectious respiratory illness or the common cold. Sleep quality is an additional mechanism that may underlie the association between stress and cold symptomatology. Although the associations between stress and sleep and cold symptomatology have been examined separately, little is known about the mechanistic role of sleep in these associations. The present study fills that void by examining archival data from the Common Cold Project (Pittsburgh Cold Study 3). The results indicate sleep quality surfaced as an indirect pathway linking stress to changes in cold severity. Additionally, better sleep was associated with greater changes in cold severity above perceived stress. These findings suggest that better sleep may be associated with less severe symptomatology. Future research should address mechanisms underlying the associations between stress, sleep, and cold symptomatology

The Association Between Discrimination and Sleep is Exacerbated in Individuals with Comorbid Chronic Health Conditions

Introduction: The consequences of recurrent, stressful daily experiences for sleep health appear intensified in individuals with pre-existing health conditions. Although discrimination has been associated with sleep outcomes, the role of comorbid chronic health conditions (CCHCs), and impact of perceived discrimination, remains unclear. The present study investigated (1) the associations between daily discrimination and sleep and (2) moderating roles of CCHCs and daily life interference and hardship. Methods: The current study utilized archival data from the Midlife in the United States (MIDUS) Study II. Participants, 174 adults (51% female, Mage=57 yrs., SD=11.5 yrs.), completed 7 days of actigraphy, sleep diary, PSQI, and CCHC-reporting measures. Models examined the moderating effects of CCHCs, daily interference, and hardship on the association between discrimination and sleep. Results: Daily discriminatory experiences predicted numerous poor sleep outcomes, exacerbated for persons with higher CCHCs. Higher comorbidity (95% CI=5.40, 68.75) exacerbated the association between discrimination and TSTactigraphy, further strengthened by perceived hardship (95% CI=-3.75, -.40) and interference (95% CI=-3.65, -.30). Number of CCHCs, qualified by perceived hardship (95% CI=.00, .04) and interference (95% CI=.01, .05), predicted diary sleep quality above discrimination. The interaction between CCHCs and hardship predicted global PSQI scores (95% CI=-.91, -.12) beyond discrimination. Conclusion: Daily experiences of discrimination are associated with decreased sleep duration and quality. These associations were stronger for individuals with multiple CCHCs. Exacerbating CCHC effects were perpetuated by perceived interference and hardships, suggesting individual emotion regulation (ER) differences. Future research should attend to sleep-related consequences of differential discrimination-informed ER by persons with CCHCs.https://scholarscompass.vcu.edu/gradposters/1042/thumbnail.jp

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The Moderating Role of Emotion Regulation in the Relationship Between Stress and Inflammatory Bowel Disease Severity Among Diagnosed Individuals

This study examined whether both perceived and objectively rated chronic stress are contributing external factors to altered Inflammatory Bowel Disease (IBD) disease severity among diagnosed individuals. This study further examined whether emotion regulation (ER) acts as an ameliorative factor within this relationship. Of additional interest to this investigation was whether objectively rated stress (acquired via the UCLA Life Stress Interview) provided a unique contribution to this relationship. Recent investigations suggest that higher levels of perceived stress may result in increased IBD severity. Further, ER deficits may be associated with increased IBD disease activity in response to both chronic and perceived stress. Participants (N=30) completed measures of perceived and chronic stress, ER, and disease severity. Multiple moderation models were examined to determine the moderating role of ER (both adaptive and maladaptive) within relationships between both perceived and chronic stress and disease severity (measured by the Harvey Bradshaw Index). No ER moderation effect was observed in the relationship(s) between objective and perceived stress ratings and IBD disease severity. However, results do suggest that adaptive and maladaptive ER significantly influence disease severity (decreased and increased respectively) independent of both objective and perceived stress ratings. These findings suggest the utility of ER-informed psychoeducation and interventions in the treatment and maintenance of IBD

Examining and Promoting Sleep Health in the Undergraduate Classroom: A Mixed-Methods Approach

Objective: Although college students are at heightened risk for sleep disturbances, healthy sleep is associated with positive physical, cognitive, psychological, and academic benefits for this group. The goals of the current study were to (1) describe sleep health in an undergraduate college sample and (2) examine the role of a class activity using self-determination theory to promote better sleep health in this group. Methods: A cohort study was conducted using data drawn from class activities conducted in two undergraduate Introduction to Psychology courses. Students were undergraduates at a mid-Atlantic public university in the United States. Total sample size was N = 224 (intervention class [n = 98], and the control class [n = 127]). Both the intervention and control classes completed the RU SATED sleep health questionnaire at the beginning and the end of the semester. The intervention class also completed a self-determination activity focused on sleep health mid-semester. Both the RU SATED questionnaires and the self-determination activities were completed via in-class responder technology. Data were de-identified and downloaded from the responder technology at the end of the semester. Mixed methods were used for data analysis including quantitative analyses and a qualitative approach using a phenomenological, inductive, and reflexive qualitative method whereby themes were allowed to emerge from the data. Results: Overall, almost 25% of the students reported never or rarely obtaining healthy sleep on average. The majority (76%) said they sometimes have healthy sleep and no students reported usually or always obtaining healthy sleep. The components of sleep health the entire sample scored highest on were timing (sleeping between 2 and 4 AM), sleep duration (between 7 and 9 h), and staying awake during the day. The areas they scored the lowest on were maintaining regular bed and wake times, spending less than 30 min awake at night, and feeling satisfied with their sleep. Qualitatively, the most frequently obtained sleep health behaviors of the intervention class were rhythmicity, prioritizing sleep, timing of sleep, and tech hygiene. The intervention class had significantly better sleep health across the entire semester and significantly better daytime alertness post-intervention. The most commonly chosen sleep health behaviors to change were sleep hygiene, tech hygiene, and stimulus control. Conclusion: We examined the classroom environment as a venue for promoting sleep health among college students. Given the popularity of Introduction to Psychology courses, this class is a promising avenue to deliver sleep health promotions to a large number of students. The implementation of a self-determination framework, as part of sleep health promotion, shows potential for creating a person-centered, strengths-based approach to health behavior change within this population

Immunochemotherapy of human colon carcinoma xenografts in nude mice using combinations of idarubicin-monoclonal antibody conjugates.

Tumour cell heterogeneity is probably a principal cause of treatment failure and represents a formidable barrier for effective antibody-targeted chemotherapy. Idarubicin (Ida), a more potent and less cardiotoxic analogue of daunomycin, has been demonstrated to specifically target and eradicate homogeneous, cloned, murine tumour cell populations in vitro and in vivo when coupled to monoclonal antibodies (MoAb); however, the antitumour activity of Ida-MoAb conjugates against human tumour xenografts remains to be established. In this study, the value of cotargeting conjugates to different human tumour-associated antigens within a solid tumour has been assessed by comparing the effects of combinations of Ida-anti-colon carcinoma MoAb conjugates with any one Ida-anti-colon carcinoma MoAb conjugate used alone. Individual Ida-MoAb conjugates have previously been evaluated for their specific binding and cytotoxicity to one of two different human colon carcinoma xenografts (Colo 205 or LIM2210) in vitro, although their efficacy alone or in combination required assessment in vivo. Combinations of the most effective Ida-MoAb conjugates were demonstrated to enable a greater number of complete tumour regressions than the most efficacious Ida-MoAb conjugate administered alone in vivo; some combinations inhibited control tumour growth by up to 95%. This study suggests that Ida-MoAb conjugates can be effective against subcutaneous human tumours in nude mice, although it is unlikely that any single conjugate will eradicate all the tumour cells in a solid tumour, and the value of 'cocktails' of drug-MoAb conjugates against some xenografts (i.e. LIM2210) appears to be limited