Sludge Management Using PLC.

The PLC is programmable logical controller used for controlling robotic arm through controlling the pneumatic operated valves. The PLC is having the output as well as input module the input module is having signals from sensors and power supply. The output module is gives the signals to pneumatic cylinders. The control panel is a panel in which all the buttons and series of relay, power supply section. Buttons are like start and stop is used to control operational cycle

Childhood tuberculosis is associated with decreased abundance of T cell gene transcripts and impaired T cell function

The WHO estimates around a million children contract tuberculosis (TB) annually with over 80 000 deaths from dissemination of infection outside of the lungs. The insidious onset and association with skin test anergy suggests failure of the immune system to both recognise and respond to infection. To understand the immune mechanisms, we studied genome-wide whole blood RNA expression in children with TB meningitis (TBM). Findings were validated in a second cohort of children with TBM and pulmonary TB (PTB), and functional T-cell responses studied in a third cohort of children with TBM, other extrapulmonary TB (EPTB) and PTB. The predominant RNA transcriptional response in children with TBM was decreased abundance of multiple genes, with 140/204 (68%) of all differentially regulated genes showing reduced abundance compared to healthy controls. Findings were validated in a second cohort with concordance of the direction of differential expression in both TBM (r2 = 0.78 p = 2x10-16) and PTB patients (r2 = 0.71 p = 2x10-16) when compared to a second group of healthy controls. Although the direction of expression of these significant genes was similar in the PTB patients, the magnitude of differential transcript abundance was less in PTB than in TBM. The majority of genes were involved in activation of leucocytes (p = 2.67E-11) and T-cell receptor signalling (p = 6.56E-07). Less abundant gene expression in immune cells was associated with a functional defect in T-cell proliferation that recovered after full TB treatment (p<0.0003). Multiple genes involved in T-cell activation show decreased abundance in children with acute TB, who also have impaired functional T-cell responses. Our data suggest that childhood TB is associated with an acquired immune defect, potentially resulting in failure to contain the pathogen. Elucidation of the mechanism causing the immune paresis may identify new treatment and prevention strategies

Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells

<p>Abstract</p> <p>Background</p> <p>Microglia are one of the main cell types to be productively infected by HIV-1 in the central nervous system (CNS). Leukotriene B₄(LTB₄) and cysteinyl-leukotrienes such as LTC₄are some of the proinflammatory molecules produced in infected individuals that contribute to neuroinflammation. We therefore sought to investigate the role of leukotrienes (LTs) in HIV-1 infection of microglial cells.</p> <p>Methods</p> <p>To evaluate the role of LTs on HIV-1 infection in the CNS, monocyte-derived microglial-like cells (MDMis) were utilized in this study. Leukotriene-treated MDMis were infected with either fully replicative brain-derived HIV-1 isolates (YU2) or R5-tropic luciferase-encoding particles in order to assess viral production and expression. The efficacy of various steps of the replication cycle was evaluated by means of p24 quantification by ELISA, luciferase activity determination and quantitative real-time polymerase chain reaction (RT-PCR).</p> <p>Results</p> <p>We report in this study that virus replication is reduced upon treatment of MDMis with LTB₄and LTC₄. Additional experiments indicate that these proinflammatory molecules alter the pH-independent entry and early post-fusion events of the viral life cycle. Indeed, LT treatment induced a diminution in integrated proviral DNA while reverse-transcribed viral products remained unaffected. Furthermore, decreased C-C chemokine receptor type 5 (CCR5) surface expression was observed in LT-treated MDMis. Finally, the effect of LTs on HIV-1 infection in MDMis appears to be mediated partly via a signal transduction pathway involving protein kinase C.</p> <p>Conclusions</p> <p>These data show for the first time that LTs influence microglial cell infection by HIV-1, and may be a factor in the control of viral load in the CNS.</p

Soy Protein Isolate/Poly(ethylene oxide) Films

Films were prepared by casting and drying alkaline aqueous film-forming solutions of soy protein isolate (SPI). Four additional types of films were made by combining SPI with poly(ethylene oxide) (PEO) in SPI to PEO ratios of 19:1, 9:1, 4:1, and 1.5:1 (w/w). Glycerin was added to all film-forming solutions as a plasticizer at 60% of total solid weight. Addition of PEO decreased film tensile strength (TS) and increased elongation at break (E). TS values ranged from 1.4 to 3.9 MPa and E values ranged from 83 to 152%. Water vapor permeability of the films ranged from 3.0 x 10-9 to 4.0 x 10-9 g/m∙sec∙Pa. Scanning electron micrographs of film cross sections showed an increase in the inferior texture with increasing amounts of PEO in the films

Heat Curing of Soy Protein Films

Modification of soy protein film properties by heat-curing was studied. Glycerin-plasticized films were cast from alkaline aqueous solutions of soy protein isolate. Films were heated at 80 or 95°C for 2, 6, 14, or 24 h. Tensile strength (TS), elongation at break (E), moisture content (MC), water solubility (WS), water vapor permeability (WVP), and color of heated and control films were measured. Heated films had increased TS and +b (yellowness) Hunter color values and reduced E, MC, WS, and WVP values. These effects were enhanced as heating time and temperature increased

Meaningfulandunexpandedsharesforvisualsecretsharingschemes

In today's internet world it is very essential to secretly share biometric data stored in the central database. There are so many options to secretly share biometric data using cryptographic computation. This work reviews and applies a perfectly secure method to secretly share biometric data, for possible use in biometric authentication and protection based on concept of visual cryptography. The basic concept of proposed approach is to secretly share private image into two meaningful and unexpanded shares (sheets) that are stored in two separate database servers such that decryption can be performed only when both shares are simultaneously available; at the same time, the individual share do not open identity of the private image

Inhibition of Red Discoloration in Cooked, Vacuum Packaged Bratwurst

A red discoloration sometimes occurs in vacuum-packaged bratwurst during storage. We attempted to characterize the red pigment and determine the effect of pH, cooking temperature, and sodium lactate on its incidence. Myoglobin was the red pigment in discolored samples. Myoglobin levels were lower in samples at pH (5.5) or cooked to 74°C. Discoloration was associated with microbial growth. Frozen samples had no red discoloration after 4 wks. Discoloration was not observed in bratwurst containing 2% sodium lactate and cooked to 74°C. In preventing red discoloration cooking to 74°C without lactate was as effective as cooking to 68°C with lactate

Kinetics of oxidation of cuprous benzoate/toluate and cobaltous benzoate in benzoic/toluic acid melts; measurement of interfacial area in melts in agit

The kinetics of oxidation of cuprous and cobaltous benzoate in benzoic acid melt; and cuprous o-toluate and cuprous p-toluate in the correspond The oxidation of cobaltous benzoate in benzoic acid melt was found to be zero order with respect to oxygen and first order with respect to cobaltous co The values of a, obtained from oxidation of cuprous and cobaltous benzoate in melts of benzoic acid at 150°C, which exhibit very different kineti

Biuret: a Potential Burning Rate Suppressant in Ammonium Chlorate(VII) Based Composite Propellants

Several composite propellant compositions containing various concentrations of biuret, a new burning rate suppressant, were formulated and studied to optimize the concentration of biuret in the composite propellant. Biuret was used here for the first time in a composite propellant as a burning rate suppressant. The theoretical properties of the compositions containing different concentrations of biuret were computed by using the NASA CEC-71 programme and the burning rate performances were evaluated. In addition, the sensitivity, thermal and mechanical properties of the compositions were also evaluated. The composition containing ammonium chlorate(VII) (AP) 65%, Al 15%, binder 20% and biuret 0-6% over the batch were prepared. The composition containing 6% biuret over the batch was insensitive to friction and impact. As the amount of biuret was increased, the energy, burning rate and sensitivity decreased, whilst the auto ignition temperature increased. The formulation containing 4% biuret over the batch was found to be the optimum with respect to energy, burning rate, pressure index, and sensitivity