The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium

Research Areas: Agriculture ; Veterinary Sciences ; ZoologyAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium. Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and gelatinolytic activity, as well as the potential inhibitory e ect of sivelestat on ELA-induced COL1 in equine endometrium. Endometrial explants from follicular (FP) and mid-luteal (MLP) phases were treated for 24 or 48 h with ELA, sivelestat, and their combination. Transcripts of COL1A2, MMP2, and MMP9 were evaluated by qPCR; COL1 protein relative abundance by Western blot, and MMP-2 and MMP-9 gelatinolytic activity by zymography. In response to ELA treatment, there was an increase in MMP2 mRNA transcription (24 h) in active MMP-2 (48 h), both in FP, and in MMP9 transcripts in FP (48 h) and MLP (24 h) (p < 0.05). Sivelestat inhibited ELA-induced COL1A2 transcripts in FP (24 h) and MLP (24 h, 48 h) (p < 0.05). The sivelestat inhibitory e ect was detected in MMP9 transcripts in FP at 48 h (p < 0.05), but proteases activity was unchanged. Thus, MMP-2 and MMP-9 might be implicated in endometrium fibrotic response to ELA. In mare endometrium, sivelestat may decrease ELA-induced COL1 deposition and hinder endometrosis development.info:eu-repo/semantics/publishedVersio

Wplyw podczyszczonej gnojowicy trzody chlewnej na zawartosc i sklad prochnicy w glebie lekkie

Po 15 latach stosowania gnojowicy trzody chlewnej, podczyszczonej poprzez składowanie na lagunach, określono zawartość i skład związków próchnicznych w glebie brunatnej kwaśnej wytworzonej z piasku słabogliniastego. Stwierdzono, że intensywne nawożenie gnojowicą podczyszczoną o wąskim stosunku C:N (0.87) i wysokim udziale N-NH₄⁺ w N ogółem (72%) powoduje obniżenie zawartości próchnicy w warstwie ornej (próchnicznej) i zwiększenie ilości związków próchnicznych w poziomie brunatnienia. Gnojowica powodowała zwiększenie zawartości frakcji kwasów huminowych a zmniejszenie zawartości humin w poziomie próchnicznym gleb.The humus content and composition of humus in acid brown soil developed with weakly loamy sand after 15 years of applying of the cleared pig slurry by the lagoon storage were determined. There was found that intensive fertilization of the cleared pig slurry with the narrow relation of C:N (0.87) and the high participation of N-NH₄ in total-N (72%) decreased humus content in the arable layer and increased humus compounds content in the brown horizon. The slurry increased humus acids content and decreased humins content in the humus horizon