Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration.

Collective cell migration is fundamental for life and a hallmark of cancer. Neural crest (NC) cells migrate collectively, but the mechanisms governing this process remain controversial. Previous analyses in Xenopus indicate that cranial NC (CNC) cells are a homogeneous population relying on cell-cell interactions for directional migration, while chick embryo analyses suggest a heterogeneous population with leader cells instructing directionality. Our data in chick and zebrafish embryos show that CNC cells do not require leader cells for migration and all cells present similar migratory capacities. In contrast, laser ablation of trunk NC (TNC) cells shows that leader cells direct movement and cell-cell contacts are required for migration. Moreover, leader and follower identities are acquired before the initiation of migration and remain fixed thereafter. Thus, two distinct mechanisms establish the directionality of CNC cells and TNC cells. This implies the existence of multiple molecular mechanisms for collective cell migration.D11I1096 Fondo de Fomento al Desarrollo Científico y TecnológicoThis is the final version of the article. It first appeared from Cell Press via httsp://doi.org/10.1016/j.celrep.2016.04.06

Parental magma and emplacement of the stratiform Uitkomst Complex, South Africa

The Uitkomst Complex, a Ni–Cu–PGE–Cr-mineralized layered basic intrusion situated approximately 200 km due east of Pretoria, South Africa, hosts commercially viable magmatic base-metal sulfide deposits. The Complex has a concordant 207Pb/206Pb zircon age of 2044 ± 8 Ma, suggesting that it is coeval with the Rustenburg Layered Suite (RLS) of the Bushveld Complex. Chemical modeling, using summation and least-squares optimization techniques, provides evidence that the boninitic Bushveld B1 magma is parental to both the lower ultrabasic and upper basic layered series of the Uitkomst Complex. The layered series crystallized in two stages, i.e., a lower conduit and an upper closed-system stage. The tubular shape of the Uitkomst Complex is the result of the intersection of a near-horizontal bedding plane fault with an existing vertical fracture zone under tensional conditions. During the conduit stage, a combination of magma mixing, contamination and flow dynamics may have facilitated sulfide formation and segregation. The identification of Bushveld B1 magma as the major parental magma of the Uitkomst Complex has significance in the exploration for similarly mineralized sub-RLS intrusive bodies