Cougars (Puma concolor) in The Northwest Territories and Wood Buffalo National Park

Extralimital reports of cougars (Puma concolor) at the northern limits of their range are rare. We documented at least 21 individual occurrences of cougars from the Northwest Territories and the Wood Buffalo National Park area between the years 1983 and 2000. Our evidence suggests that, at a minimum, transient cougars are regular visitors to northern Alberta and the Northwest Territories.La présence de couguars (Puma concolor) est rarement rapportée en dehors des limites septentrionales de territoire. On a documenté au moins 21 cas individuels de la présence de couguars dans les Territoires du Nord-Ouest et le parc national Wood Buffalo entre les années 1983 et 2000. Les éléments de preuve que nous apportons suggèrent que, à tout le moins, les couguars de passage sont des visiteurs réguliers du nord de l'Alberta et des Territoires du Nord-Ouest

Feeding Patterns of Barren-ground Grizzly Bears in the Central Canadian Arctic

We collected 169 grizzly bear scats between 1994 and 1997 to determine the dietary habits of barren-ground grizzly bears (Ursus arctos) inhabiting Canada's central Arctic. From personal observations and fecal analysis, we concluded that barren-ground grizzly bears lead a predominantly carnivorous lifestyle and are effective predators of caribou (Rangifer tarandus). Caribou was a predominant diet item during spring, mid-summer, and fall. During early summer, grizzly bears foraged primarily on green vegetation. Berries increased in dietary importance in late summer. Declines in the caribou population of our study area or long-term absences of caribou may threaten the local grizzly bear population.On a prélevé 169 excréments de grizzli entre 1994 et 1997 afin de déterminer les habitudes alimentaires du grizzli de Richardson (Ursus arctos) qui vit dans le centre de l'Arctique canadien. En s'appuyant sur des observations personnelles et un examen coproscopique, on conclut que le grizzli de Richardson est un animal largement carnassier et qu'il est un prédateur efficace du caribou (Rangifer tarandus). Ce dernier constituait un aliment prédominant au printemps, au milieu de l'été et en automne. Au début de l'été, le grizzli se nourrissait surtout de végétation verte. À la fin de l'été, les baies prenaient plus d'importance dans son alimentation. Le déclin de la population du caribou dans la zone d'étude ou son absence prolongée peut constituer une menace pour la population locale de grizzlis

Movements of Subadult Male Grizzly Bears, Ursus arctos, in the Central Canadian Arctic

Between May 1995 and June 1999, we equipped eight subadult male (3-5 yrs old) Grizzly Bears (Ursus arctos) with satellite radio-collars within a study area of 235,000 km2, centred 400 km northeast of Yellowknife, Northwest Territories, Canada. Subadult male annual home ranges were extraordinarily large (average = 11,407 km2, SE = 3849) due, in part, to their movement's occasional linear directionality. We believe their long-range linear movements may reflect some individuals tracking the migration of Caribou (Rangifer tarandus). Seasonal daily movement patterns were similar to adult males that were previously reported. The areas used by these bears are the largest ranges reported for any Grizzly Bears and the scale of their movements may put individual bears in contact with humans even when developments are hundreds of kilometres from the central home range of an animal

Population Viability of Barren-ground Grizzly Bears in Nunavut and the Northwest Territories

We modelled probabilities of population decline as a function of annual kill for a population of barren-ground grizzly bears (Ursus arctos) inhabiting Nunavut and the Northwest Territories, Canada. Our results suggest that the population is at risk of decline, especially if annual removal rates increase from the 42-year mean of 13.4 bears per year. Adding six bears to the mean annual kill results in a greater than 40% chance of a decrease by one-quarter in population size over the next 50 years, compared to a 10% chance with the current level of human-caused mortality. Additional mortalities may result from increased problem behaviour by bears at mine sites or hunt and exploration camps, given recent increases in human activity in the region, and may already be present as unreported mortality. We believe any increase in current harvest quotas would considerably lessen conservation prospects for the population.On a simulé les probabilités de baisse de la population en fonction du prélèvement annuel dans le cadre de la chasse pour une population de grizzlis de la toundra (Ursus arctos) habitant le Nunavut et les Territoires du Nord-Ouest, au Canada. Nos résultats suggèrent que la population risque de décliner, surtout si les taux de prélèvement augmentent par rapport à la moyenne établie sur 42 ans qui est de 13,4 ours par an. Le fait d'ajouter 6 ours au prélèvement de chasse annuel augmente à plus de 40 % le risque que la population décline d'un quart au cours des prochains 50 ans, par rapport à 10 % dans le cas du niveau actuel de mortalité provoquée par les humains. Vu l'augmentation récente de l'activité anthropique dans la région, d'autres individus pourraient être abattus à cause du nombre croissant de comportements problématiques des ours résidant à des sites miniers et à des campements d'exploration, et il est possible que ce phénomène existe déjà mais que les morts ne soient pas rapportées. Notre opinion est que toute augmentation des quotas actuels de prélèvement réduirait considérablement les perspectives de conservation pour la population

Population Viability of Barren-ground Grizzly Bears in Nunavut and the Northwest Territories

ABSTRACT. We modelled probabilities of population decline as a function of annual kill for a population of barren-ground grizzly bears (Ursus arctos) inhabiting Nunavut and the Northwest Territories, Canada. Our results suggest that the population is at risk of decline, especially if annual removal rates increase from the 42-year mean of 13.4 bears per year. Adding six bears to the mean annual kill results in a greater than 40 % chance of a decrease by one-quarter in population size over the next 50 years, compared to a 10 % chance with the current level of human-caused mortality. Additional mortalities may result from increased problem behaviour by bears at mine sites or hunt and exploration camps, given recent increases in human activity in the region, and may already be present as unreported mortality. We believe any increase in current harvest quotas would considerably lessen conservation prospects for the population. Key words: Arctic, demography, grizzly bear, harvest, Northwest Territories, Nunavut, population viability, PVA, Ursus arctos RÉSUMÉ. On a simulé les probabilités de baisse de la population en fonction du prélèvement annuel dans le cadre de la chasse pour une population de grizzlis de la toundra (Ursus arctos) habitant le Nunavut et les Territoires du Nord-Ouest, au Canada. Nos résultats suggèrent que la population risque de décliner, surtout si les taux de prélèvement augmentent par rapport à la moyenne établie sur 42 ans qui est de 13,4 ours par an. Le fait d’ajouter 6 ours au prélèvement de chasse annuel augmente à plus de 40 % le risque que la population décline d’un quart au cours des prochains 50 ans, par rapport à 10 % dans le cas du niveau actuel de mortalité provoquée par les humains. Vu l’augmentation récente de l’activité anthropique dans la région, d’autres individu

PET-BIDS, an extension to the brain imaging data structure for positron emission tomography

The Brain Imaging Data Structure (BIDS) is a standard for organizing and describing neuroimaging datasets, serving not only to facilitate the process of data sharing and aggregation, but also to simplify the application and development of new methods and software for working with neuroimaging data. Here, we present an extension of BIDS to include positron emission tomography (PET) data, also known as PET-BIDS, and share several open-access datasets curated following PET-BIDS along with tools for conversion, validation and analysis of PET-BIDS datasets

Microscopy-BIDS: An Extension to the Brain Imaging Data Structure for Microscopy Data

The Brain Imaging Data Structure (BIDS) is a specification for organizing, sharing, and archiving neuroimaging data and metadata in a reusable way. First developed for magnetic resonance imaging (MRI) datasets, the community-led specification evolved rapidly to include other modalities such as magnetoencephalography, positron emission tomography, and quantitative MRI (qMRI). In this work, we present an extension to BIDS for microscopy imaging data, along with example datasets. Microscopy-BIDS supports common imaging methods, including 2D/3D, ex/in vivo, micro-CT, and optical and electron microscopy. Microscopy-BIDS also includes comprehensible metadata definitions for hardware, image acquisition, and sample properties. This extension will facilitate future harmonization efforts in the context of multi-modal, multi-scale imaging such as the characterization of tissue microstructure with qMRI

RERG Is a Novel ras -related, Estrogen-regulated and Growth-inhibitory Gene in Breast Cancer

Using microarray analysis, we identified a unique ras superfamily gene, termed RERG (ras-related and estrogen-regulated growth inhibitor), whose expression was decreased or lost in a significant percentage of primary human breast tumors that show a poor clinical prognosis. Importantly, high RERG expression correlated with expression of a set of genes that define a breast tumor subtype that is estrogen receptor-positive and associated with a slow rate of tumor cell proliferation and a favorable prognosis for these cancer patients. RERG mRNA expression was induced rapidly in MCF-7 cells stimulated by beta-estradiol and repressed by tamoxifen treatment. Like Ras, RERG protein exhibited intrinsic GDP/GTP binding and GTP hydrolysis activity. Unlike Ras proteins, RERG lacks a known recognition signal for COOH-terminal prenylation and was localized primarily in the cytoplasm. Expression of RERG protein in MCF-7 breast carcinoma cells resulted in a significant inhibition of both anchorage-dependent and anchorage-independent growth in vitro and inhibited tumor formation in nude mice. These features of RERG are strikingly different from most Ras superfamily GTP-binding pro-teins and suggest that the loss of RERG expression may contribute to breast tumorigenesis

Regulation of mTORC1 Signaling by pH

BACKGROUND: Acidification of the cytoplasm and the extracellular environment is associated with many physiological and pathological conditions, such as intense exercise, hypoxia and tumourigenesis. Acidification affects important cellular functions including protein synthesis, growth, and proliferation. Many of these vital functions are controlled by mTORC1, a master regulator protein kinase that is activated by various growth-stimulating signals and inactivated by starvation conditions. Whether mTORC1 can also respond to changes in extracellular or cytoplasmic pH and play a role in limiting anabolic processes in acidic conditions is not known. METHODOLOGY/FINDINGS: We examined the effects of acidifying the extracellular medium from pH 7.4 to 6.4 on human breast carcinoma MCF-7 cells and immortalized mouse embryo fibroblasts. Decreasing the extracellular pH caused intracellular acidification and rapid, graded and reversible inhibition of mTORC1, assessed by measuring the phosphorylation of the mTORC1 substrate S6K. Fibroblasts deleted of the tuberous sclerosis complex TSC2 gene, a major negative regulator of mTORC1, were unable to inhibit mTORC1 in acidic extracellular conditions, showing that the TSC1-TSC2 complex is required for this response. Examination of the major upstream pathways converging on the TSC1-TSC2 complex showed that Akt signaling was unaffected by pH but that the Raf/MEK/ERK pathway was inhibited. Inhibition of MEK with drugs caused only modest mTORC1 inhibition, implying that other unidentified pathways also play major roles. CONCLUSIONS: This study reveals a novel role for the TSC1/TSC2 complex and mTORC1 in sensing variations in ambient pH. As a common feature of low tissue perfusion, low glucose availability and high energy expenditure, acidic pH may serve as a signal for mTORC1 to downregulate energy-consuming anabolic processes such as protein synthesis as an adaptive response to metabolically stressful conditions