Letter, 1979 February 26, from John C. Gaston to Gene DeGruson

1 page, Gaston worked in the Department of Minority Studies at Wichita State University. DeGruson was a Curator for the Special Collections at the Leonard H. Axe Library

Mapping biodiversity value worldwide: combining higher-taxon richness from different groups

Maps of large-scale biodiversity are urgently needed to guide conservation, and yet complete enumeration of organisms is impractical at present. One indirect approach is to measure richness at higher taxonomic ranks, such as families. The difficulty is how to combine information from different groups on numbers of higher taxa, when these taxa may in effect have been defined in different ways, particularly for more distantly related major groups. In this paper, the regional family richness of terrestrial and freshwater seed plants, amphibians, reptiles and mammals is mapped worldwide by combining: (i) absolute family richness; (ii) proportional family richness; and (iii) proportional family richness weighted for the total species richness in each major group. The assumptions of the three methods and their effects on the results are discussed, although for these data the broad pattern is surprisingly robust with respect to the method of combination. Scores from each of the methods of combining families are used to rank the top five richness hotspots and complementary areas, and hotspots of endemism are mapped by unweighted combination of range-size rarity scores

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers

Protein self-organization is essential for the establishment and maintenance of nuclear architecture and for the regulation of gene expression. We have shown previously that the Proline-Rich Homeodomain protein (PRH/Hex) self-assembles to form oligomeric complexes that bind to arrays of PRH binding sites with high affinity and specificity. We have also shown that many PRH target genes contain suitably spaced arrays of PRH sites that allow this protein to bind and regulate transcription. Here, we use analytical ultracentrifugation and electron microscopy to further characterize PRH oligomers. We use the same techniques to show that PRH oligomers bound to long DNA fragments self-associate to form highly ordered assemblies. Electron microscopy and linear dichroism reveal that PRH oligomers can form protein–DNA fibres and that PRH is able to compact DNA in the absence of other proteins. Finally, we show that DNA compaction is not sufficient for the repression of PRH target genes in cells. We conclude that DNA compaction is a consequence of the binding of large PRH oligomers to arrays of binding sites and that PRH is functionally and structurally related to the Lrp/AsnC family of proteins from bacteria and archaea, a group of proteins formerly thought to be without eukaryotic equivalents

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers

Protein self-organization is essential for the establishment and maintenance of nuclear architecture and for the regulation of gene expression. We have shown previously that the Proline-Rich Homeodomain protein (PRH/Hex) self-assembles to form oligomeric complexes that bind to arrays of PRH binding sites with high affinity and specificity. We have also shown that many PRH target genes contain suitably spaced arrays of PRH sites that allow this protein to bind and regulate transcription. Here, we use analytical ultracentrifugation and electron microscopy to further characterize PRH oligomers. We use the same techniques to show that PRH oligomers bound to long DNA fragments self-associate to form highly ordered assemblies. Electron microscopy and linear dichroism reveal that PRH oligomers can form protein–DNA fibres and that PRH is able to compact DNA in the absence of other proteins. Finally, we show that DNA compaction is not sufficient for the repression of PRH target genes in cells. We conclude that DNA compaction is a consequence of the binding of large PRH oligomers to arrays of binding sites and that PRH is functionally and structurally related to the Lrp/AsnC family of proteins from bacteria and archaea, a group of proteins formerly thought to be without eukaryotic equivalents

Structural transformations and disordering in zirconolite (CaZrTi2O7) at high pressure

There is interest in identifying novel materials for use in radioactive waste applications and studying their behavior under high pressure conditions. The mineral zirconolite (CaZrTi2O7) exists naturally in trace amounts in diamond-bearing deep-seated metamorphic/igneous environments, and it is also identified as a potential ceramic phase for radionuclide sequestration. However, it has been shown to undergo radiation-induced metamictization resulting in amorphous forms. In this study we probed the high pressure structural properties of this pyrochlore-like structure to study its phase transformations and possible amorphization behavior. Combined synchrotron X-ray diffraction and Raman spectroscopy studies reveal a series of high pressure phase transformations. Starting from the ambient pressure monoclinic structure, an intermediate phase with P21/m symmetry is produced above 15.6 GPa via a first order transformation resulting in a wide coexistence range. Upon compression to above 56 GPa a disordered metastable phase III with a cotunnite-related structure appears that is recoverable to ambient conditions. We examine the similarity between the zirconolite behavior and the structural evolution of analogous pyrochlore systems under pressure.<br/

Promotional benefits of 99-ending prices: the moderating role of intuitive and analytical decision style

Evidence suggests that retailers use 99-ending prices as a promotional technique. This paper explores the consumer perceived benefits of such promotional practices and the information processing conditions under which hedonic benefits are higher (lower) than utilitarian ones. In line with the benefit framework of sales promotion proposed by Chandon, Wansink, and Laurent (2000), 99-ends are not expected to be strictly associated to monetary savings benefit but also to a more hedonic benefit, fulfilling consumer’s needs for exploration. In addition, the analytic or intuitive consumer decision style (Mantel & Kardes, 1999) is likely to activate either the utilitarian benefit of savings or the hedonic benefit of exploration with more strength. The analysis of an on-line survey among 317 shoppers shows that both perceived savings and exploration benefits have a positive impact on proneness to buy 99-ends products with the saving benefits being stronger (weaker) for analytical decision makers (intuitive decision makers). The findings contribute to the better understanding of the image effect of 99-ends underlying its individual differences and help retailers and also pricing managers in their use of 99-ends as a promotional technique

Behavioral Ecology of Two Teal Species (Blue-Winged Teal, Anas discors, and Green-Winged Teal, Anas crecca) Overwintering in Marshes of Coastal Louisiana, USA

Feeding and other dominant activities of Blue-winged Teal (BWT, Anas discors) and Green-winged Teal (GWT, Anas crecca) were compared from October 1987 to March 1988 in southwestern Louisiana, USA. Three observation towers were constructed near similar intermediate marsh habitats in areas where BWT and GWT concentrated for feeding. These observation towers allowed activities of the two species to be compared throughout the nonbreeding season. Although BWT and GWT often fed together, time spent in various activities differed. Feeding was the most frequent activity of both BWT(64.5%) and GWT (55.3%), but BWT spent more time feeding (P \u3c 0.01) and alert (P \u3c 0.05), but spent less (P \u3c 0.01) time resting than GWT. Within each species there were differences in activity budgets among daily time blocks and among months, but few differences among the three habitats studied. Temperature and light intensity were correlated with resting (+), feeding (-), locomotion (-), and preening (+). Daily and monthly activity budgets of BWT and GWT were similar, as were ingested foods, suggesting that these two species used the study areas primarily for foraging, and left the areas for other activities. Predation and diminished resources during late winter may have affected activities of BWT and GWT as well

Behavioral Ecology of Two Teal Species (Blue-Winged Teal, Anas discors, and Green-Winged Teal, Anas crecca) Overwintering in Marshes of Coastal Louisiana, USA

Feeding and other dominant activities of Blue-winged Teal (BWT, Anas discors) and Green-winged Teal (GWT, Anas crecca) were compared from October 1987 to March 1988 in southwestern Louisiana, USA. Three observation towers were constructed near similar intermediate marsh habitats in areas where BWT and GWT concentrated for feeding. These observation towers allowed activities of the two species to be compared throughout the nonbreeding season. Although BWT and GWT often fed together, time spent in various activities differed. Feeding was the most frequent activity of both BWT(64.5%) and GWT (55.3%), but BWT spent more time feeding (P \u3c 0.01) and alert (P \u3c 0.05), but spent less (P \u3c 0.01) time resting than GWT. Within each species there were differences in activity budgets among daily time blocks and among months, but few differences among the three habitats studied. Temperature and light intensity were correlated with resting (+), feeding (-), locomotion (-), and preening (+). Daily and monthly activity budgets of BWT and GWT were similar, as were ingested foods, suggesting that these two species used the study areas primarily for foraging, and left the areas for other activities. Predation and diminished resources during late winter may have affected activities of BWT and GWT as well

Beam hardening artifact reduction using dual energy computed tomography: implications for myocardial perfusion studies

Background: Myocardial perfusion computed tomography (CTP) using conventional single energy (SE) imaging is influenced by the presence of beam hardening artifacts (BHA), occasionally resembling perfusion defects and commonly observed at the left ventricular posterobasal wall (PB). We therefore sought to explore the ability of dual energy (DE) CTP to attenuate the presence of BHA. Methods: Consecutive patients without history of coronary artery disease who were referred for computed tomography coronary angiography due to atypical chest pain and a normal stress-rest SPECT and had absence or mild coronary atherosclerosis constituted the study population. The study group was acquired using DE and the control group using SE imaging. Results: Demographical characteristics were similar between groups, as well as the heart rate and the effective radiation dose. Myocardial signal density (SD) levels were evaluated in 280 basal segments among the DE group (140 PB segments for each energy level from 40 keV to 100 keV; and 140 reference segments), and in 40 basal segments (at the same locations) among the SE group. Among the DE group, myocardial SD levels and myocardial SD ratio evaluated at the reference segment were higher at low energy levels, with significantly lower SD levels at increasing energy levels. Myocardial signal-to-noise ratio was not significantly influenced by the energy level applied, although 70 keV was identified as the energy level with the best overall signal-to-noise ratio. Significant differences were identified between the PB segment and the reference segment among the lower energy levels, whereas at ≥ 70 keV myocardial SD levels were similar. Compared to DE reconstructions at the best energy level (70 keV), SE acquisitions showed no significant differences overall regarding myocardial SD levels among the reference segments. Conclusions: Beam hardening artifacts that influence the assessment of myocardial perfusion can be attenuated using DE at 70 keV or higher.Fil: Rodriguez Granillo, Gaston Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Diagnóstico Maipú; ArgentinaFil: Carrascosa, Patricia. Diagnóstico Maipú; ArgentinaFil: Cipriano, Silvia. Diagnóstico Maipú; ArgentinaFil: De Zan, Macarena. Diagnóstico Maipú; ArgentinaFil: Deviggiano, Alejandro. Diagnóstico Maipú; ArgentinaFil: Capunay, Carlos. Diagnóstico Maipú; ArgentinaFil: Cury, Ricardo C.. Miami Cardiac and Vascular Institute and Baptist Health; Estados Unido