Radiative Corrections to πl2\pi_{l2} and Kl2K_{l2} Decays

We reexamine radiative corrections to $\pi_{l2}$ and $K_{l2}$ decays. We perform a matching calculation, including vector and axial vector resonances as explicit degrees of freedom in the long distance part. By considering the dependence on the matching scale and on the hadronic parameters, and by comparing with model independent estimates, we scrutinize the model dependence of the results. For the pseudoscalar meson decay constants, we extract the values f_pi = (92.1 \pm 0.3) MeV and f_K = (112.4 \pm 0.9) MeV. For the ratios R_pi and R_K of the electronic and muonic decay modes, we predict R_pi = (1.2354 \pm 0.0002) 10^{-4} and R_K = (2.472 \pm 0.001) 10^{-5}.Comment: 8 pages, Latex, uses epsfig. Revised version: Major changes in the presentaion, but no changes in the results. To appear in Phys. Lett.

From the lab to the industrial scale: EBC thermal spray powders

The quality and performance of the feedstock material plays a key role in achieving a robust Environmental Barrier Coating (EBC) for Ceramic Matrix Composites (CMCs). As CMCs become commercialized there is a great need to implement the industrial-scale manufacture of EBC thermal spray powders from its current position as a laboratory-scale process. The challenges and the necessary development required to design a manufacturing process route from the raw materials to the RE-silicates thermal spray powder will be discussed. The fused and crushed (F&C) manufacturing process, including the new morphology of the agglomerated and sintered (A&S) RE-silicate powders, will be presented. The results of the influences of the process parameters on the evolved phases, particle size distribution, homogeneity, chemistry, purity and morphology will be discussed in detail. We will show that the selection of the best-performing material can be made based on the results from the correlations between flowability, apparent density, mechanical strength and morphology

How Is CYP17A1 Activity Altered in Autism? A Pilot Study to Identify Potential Pharmacological Targets.

Background: Increasing evidence exists that higher levels of androgens can be found in individuals with autism. Evidence yields to a susceptible role of Cytochrome P450 17A1 (CYP17A1) with its catalyzation of the two distinct types of substrate oxidation by a hydroxylase activity (17-alpha hydroxylase) and C17/20 lyase activity. However, to what extent steps are altered in affected children with autism versus healthy controls remains to be elucidated. Methods: Urine samples from 48 boys with autism (BMI 19.1 ± 0.6 kg/m2, age 14.2 ± 0.5 years) and a matched cohort of 48 healthy boys (BMI 18.6 ± 0.3 kg/m2, 14.3 ± 0.5 years) as well as 16 girls with autism (BMI 17.5 ± 0.7 kg/m2, age 13.8 ± 1.0 years) and a matched cohort of 16 healthy girls (BMI 17.2 ± 0.8 kg/m2, age 13.2 ± 0.8 years) were analyzed for steroid hormone metabolites by gas chromatography-mass spectrometry. Results: The activity of 17-alpha Hydroxylase increased by almost 50%, whereas activity of 17/20 Lyase activity increased by around 150% in affected children with autism. Furthermore, the concentration of Cortisol was higher as compared to the average increase of the three metabolites TH-Corticosterone, 5α-TH-Corticosterone and TH-11β-DH-Corticosterone, indicating, in addition, a stimulation by the CRH-ACTH system despite a higher enzymatic activity. Discussion: As it was shown that oxidative stress increases the 17/20-lyase activity via p38α, a link between higher steroid hormone levels and oxidative stress can be established. However, as glucocorticoid as well as androgen metabolites showed higher values in subjects affected with autism as compared to healthy controls, the data indicate, despite higher CYP17A1 activity, the presence of increased substrate availability in line with the Cholesterol theory of autism

Slow growth rate triggered transition to a pseudohyphal lifestyle of the protein production host Pichia pastoris

Specific growth rate is an important process control parameter for industrial protein production. In the widely used yeast protein production host Pichia pastoris, growth rate is known to significantly impact protein expression and secretion [1]. In that regard, glucose-limited chemostat cultivations carried out over a wide range of specific growth rates have revealed that slow growth rates can trigger a pseudohyphal phenotype in P. pastoris [2]. Such phenotypes are undesirable during large-scale protein production processes since they can lead to foam production. In Saccharomyces cerevisiae pseudohyphal growth is controlled by FLO11, a member of the FLO gene family, which is a group of genes encoding cell surface proteins responsible for conferring a diverse array of adhesion-related phenotypes and reported to be controlled by epigenetic mechanisms. P. pastoris also carries a number of FLO genes but their functions and regulatory patterns are yet unknown. Thus, we set out to investigate this gene family to shed some light on how pseudohyphal growth and other adhesion phenotypes are triggered and regulated in P. pastoris. Please click Additional Files below to see the full abstract

Androgens Tend to Be Higher, but What about Altered Progesterone Metabolites in Boys and Girls with Autism?

BACKGROUND Evidence exists that steroid hormones are altered in individuals with autism, especially androgens. Despite lower prevalence in girls than boys, evidence of potential alterations in progesterone metabolites is sparse, so the aim of this study was to elucidate different progesterone metabolites in affected children with autism versus healthy controls. MATERIAL AND METHODS Circadian urine samples from 48 boys and 16 girls with autism spectrum disorders and a matched case-control group were analysed for progesterone metabolites by gas chromatography-mass spectrometry and normalised for creatinine excretion. RESULTS In boys with autism, the majority of progesterone metabolites were reduced, such as progesterone, 6a-OH-3a5b-TH-progesterone, or 20a-DH-progesterone (p < 0.01 for all). In girls with autism, a similar pattern of reduction in progesterone metabolites was detected; however, potentially due to the relatively small sample, this pattern was only detectable on the level of a trend. DISCUSSION As stated, androgen levels are higher in boys and girls with autism, but evidence for progesterone metabolites is much sparser. The pattern of a decrease in progesterone metabolites suggests the existence of an altered routing of steroid metabolites, probably in combination with a dysregulation of the HPAG axis. As, recently, increased CYP17A1 activity has been suggested, the stronger routing towards androgens is further implied in line with our findings of lower progesterone concentrations in boys and girls with autism than healthy controls

The Brain at High Altitude: From Molecular Signaling to Cognitive Performance

The brain requires over one-fifth of the total body oxygen demand for normal functioning. At high altitude (HA), the lower atmospheric oxygen pressure inevitably challenges the brain, affecting voluntary spatial attention, cognitive processing, and attention speed after short-term, long-term, or lifespan exposure. Molecular responses to HA are controlled mainly by hypoxia-inducible factors. This review aims to summarize the cellular, metabolic, and functional alterations in the brain at HA with a focus on the role of hypoxia-inducible factors in controlling the hypoxic ventilatory response, neuronal survival, metabolism, neurogenesis, synaptogenesis, and plasticity

Holzreste von spätglazialen Kiefern aus der tiefgründigen und tonreichen Permanentrutschung ‚Spiegelberg’, Kanton Schwyz (Schweiz)

Die Hauptstrasse Nr. 8 zwischen Schwyz und Sattel verläuft durch aktive und tiefgründige Permanentrutschungen. Im lehmigen, matrix-gestützten Gehängeschutt der Rutschung ‚Spiegelberg’ wurde zwischen 1979–81 die Gütschbrücke erstellt (LK: 690.314/211.943; 670 m ü.M.) und unter Anwendung von Gründungsschutzschächten im unterlagernden Fels fundiert. Beim Aushub des Schutzschachtes für den Pfeiler WL-Nord wurden in der Tiefe von 25 m bzw. 38 m unter Oberkante Terrain zwei Nadelbaumfragmente gefunden. Letzterer Holzfund lag wenige Meter über der Felsoberfläche. Die 14C-Altersdatierung der Holzfunde (beide Pinus sylvestris) ergaben kalibrierte Altersspannen zwischen 11.690–11.270 cal. a BP (2s) am Übergang vom Grönland Stadial 1 (GS-1; ‚Jüngere Dryas’) zum Holozän bzw. 13.830–13.640 cal. a BP (2s) zu Beginn der spätglazialen Wärmeschwankung GI-1c (Grönland Interstadial 1c; ‚Allerød’). Die vorliegenden Daten zeigen, dass die Hanginstabilitäten bei ‚Spiegelberg’ nach dem Zerfall des letzteiszeitlichen Muota/Reussgletschers zu Beginn des Spätglazials eingesetzt haben mussten, und die Waldkiefer schon kurz nach den Kälterückschlägen des GI-1d (‚Aegelsee-Schwankung’) bzw. des GS-1 am nördlichen Alpenrand präsent war.researc

Microbial Cell Factories / Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Background: The methylotrophic yeast Pichia pastoris is frequently used for the production of recombinant proteins. However, expression levels can vary depending on the target protein. Allowing for simultaneous regulation of many genes, which may elicit a desired phenotype like increased protein production, overexpression of transcription factors can be used to overcome expression bottlenecks. Here, we present a novel P. pastoris transcription factor currently annotated as Aft1, activator of ferrous transport. Results: The promoter regions of key secretory P. pastoris genes were screened for fungal transcription factor binding sites, revealing Aft1 as an interesting candidate for improving secretion. Genome wide analysis of transcription factor binding sites suggested Aft1 to be involved in the regulation of many secretory genes, but also indicated possible novel functions in carbohydrate metabolism. No Aft binding sites were found in promoters of characteristic iron homeostasis genes in P. pastoris. Microarrays were used to study the Aft1 regulon in detail, confirming Aft1 involvement in the regulation of carbon-responsive genes, and showing that iron regulation is dependent on FEP1, but not AFT1 expression levels. The positive effect of AFT1 overexpression on recombinant protein secretion was demonstrated for a carboxylesterase from Sphingopyxis sp. MTA144, for which secretion was improved 2.5-fold in fed batch bioreactor cultivations. Conclusion: This study demonstrates that the transcription factor Aft1 can be used to improve recombinant protein secretion in P. pastoris. Furthermore, we discovered possible novel functions of Aft1 in carbohydrate metabolism and provide evidence arguing against a direct role of Aft1 in P. pastoris iron regulation

Radiative Tau Decays with One Pseudoscalar Meson

We have calculated the decay $\tau \rightarrow \nu \pi(K) \gamma$. We present the photon energy spectrum, the meson-photon invariant mass spectrum and the integrated rate as a function of a photon energy cut or an invariant mass cut. Both the internal bremsstrahlung and the structure dependent radiation have been taken into account. To this aim we have parametrized the form factors $F_V$ and $F_A$, which determine the structure dependent radiation. Observables especially suited for the measurement of the structure dependent form factors are found and implications on the width of the $a_1$ discussed.Comment: p.20, TTP93-1, LaTe