Exopolysaccharide-producing Lacticaseibacillus paracasei strains isolated from kefir as starter for functional dairy products

Exopolysaccharides (EPS) produced by lactic acid bacteria are molecules of great interest for the dairy food industry. Lacticaseibacillus paracasei CIDCA 8339, CIDCA 83123, and CIDCA 83124 are potentially probiotic strains isolated from kefir grains whose EPS-production on MRS broth is dependent on incubation temperature. The aim of the present work is to evaluate the effect of fermentation temperature on the characteristics of EPS produced in milk by L. paracasei strains and the consequent impact on the rheological properties of the fermented products. Additionally, the protective effect of these EPS against Salmonella infection was evaluated in vitro. Acid gels with each strain were obtained by milk fermentation at 20°C, 30°C, and 37°C evidencing for all the strains a reduction in growth and acidification rate at lower temperature. Lacticaseibacillus paracasei CIDCA 83123 showed low fermentation rate at all temperatures requiring between 3 and 8 days to obtain acids gels, whereas CIDCA 8339 and 83124 needed between 24 and 48 h even when the temperature was 20°C. Fermentation temperature led to changes in crude EPS characteristics of the three strains, observing an increase in the relative amount of the high molecular weight fraction when the fermentation temperature diminished. Additionally, EPS83124 and EPS83123 presented modifications in monosaccharide composition, with a reduction of rhamnose and an increase of amino-sugars as temperature rise. These changes in the structure of EPS83124 resulted in an increase of the apparent viscosity of milks fermented at 20°C (223 mPa.s) and 30°C (217 mPa.s) with respect to acid gels obtained at 37°C (167 mPa.s). In order to deepen the knowledge on EPS characteristics, monosaccharide composition of low and high molecular weight EPS fractions were evaluated. Finally, it was evidenced that the preincubation of intestinal epithelial cells Caco-2/TC-7 with EPS8339 and EPS83124 partially inhibit the association and invasion of Salmonella. In light of these results, it can be concluded that the selection of the EPS-producing strain along with the appropriate fermentation conditions could be an interesting strategy to improve the technological properties of these L. paracasei fermented milks with potential protective effects against intestinal pathogens.The present work was supported by CONICET (PIP 2786), Universidad Nacional de La Plata (UNLP 18/X813), ANPCyT (PICT 2020-03973 and PICT 2020-3239), and the Basque Government (IT1662-22 and PIBA 2020_1_0032)

Shell Evolution towards Ni 78: Low-Lying States in Cu 77

The level structure of the neutron-rich Cu77 nucleus is investigated through β-delayed γ-ray spectroscopy at the Radioactive Isotope Beam Factory of the RIKEN Nishina Center. Ions of Ni77 are produced by in-flight fission, separated and identified in the BigRIPS fragment separator, and implanted in the WAS3ABi silicon detector array, surrounded by Ge cluster detectors of the EURICA array. A large number of excited states in Cu77 are identified for the first time by correlating γ rays with the β decay of Ni77, and a level scheme is constructed by utilizing their coincidence relationships. The good agreement between large-scale Monte Carlo shell model calculations and experimental results allows for the evaluation of the single-particle structure near Ni78 and suggests a single-particle nature for both the 5/21- and 3/21- states in Cu77, leading to doubly magic Ni78. © 2017 American Physical Society

Completing the nuclear reaction puzzle of the nucleosynthesis of 92Mo

One of the greatest questions for modern physics to address is how elements heavier than iron are created in extreme, astrophysical environments. A particularly challenging part of that question is the creation of the so-called p-nuclei, which are believed to be mainly produced in some types of supernovae. The lack of needed nuclear data presents an obstacle in nailing down the precise site and astrophysical conditions. In this work, we present for the first time measurements on the nuclear level density and average strength function of $^{92}$Mo. State-of-the-art p-process calculations systematically underestimate the observed solar abundance of this isotope. Our data provide stringent constraints on the $^{91}$Nb$(p,{\gamma})^{92}$Mo reaction rate, which is the last unmeasured reaction in the nucleosynthesis puzzle of $^{92}$Mo. Based on our results, we conclude that the $^{92}$Mo abundance anomaly is not due to the nuclear physics input to astrophysical model calculations.Comment: Submitted to PR

Nuclear level densities and γ−\gamma-ray strength functions of 111,112,113^{111,112,113}Sn isotopes studied with the Oslo method

The $^{111,112,113}$Sn isotopes have been studied with ($p,d \gamma$), ($p,p^{\prime} \gamma$), and ($d,p \gamma$) reactions to extract the nuclear level densities (NLDs) and $\gamma$-ray strength functions (GSFs) of these nuclei below the neutron separation energy by means of the Oslo method. The experimental NLDs for all three nuclei demonstrate a trend compatible with the constant-temperature model below the neutron separation energy while also being in good agreement with the NLDs of neighboring Sn isotopes, obtained previously with the Oslo-type and neutron evaporation experiments. The extracted microcanonical entropies yield $\approx 1.5$ $k_B$ entropy of a valence neutron in both $^{111}$Sn and $^{113}$Sn. Moreover, the deduced microcanonical temperatures indeed suggest a clear constant-temperature behavior above $\approx$ 3 MeV in $^{111,113}$Sn and above $\approx$ 4.5 MeV in $^{112}$Sn. We observe signatures for the first broken neutron pairs between 2 and 4 MeV in all three nuclei. The GSFs obtained with the Oslo method are found to be in good agreement below the neutron threshold with the strengths of $^{112,114}$Sn extracted in the ($p,p^{\prime}$) Coulomb excitation experiments.Comment: 13 pages, 9 figure

Statistical properties of the well deformed 153,155^{153,155}Sm nuclei and the scissors resonance

The Nuclear Level Densities (NLDs) and the $\gamma$-ray Strength Functions ($\gamma$SFs) of $^{153,155}$Sm have been extracted from (d,p$\gamma$) coincidences using the Oslo method. The experimental NLD of $^{153}$Sm is higher than the NLD of $^{155}$Sm, in accordance with microscopic calculations. The $\gamma$SFs of $^{153,155}$Sm are in fair agreement with QRPA calculations based on the D1M Gogny interaction. An enhancement is observed in the $\gamma$SF for both $^{153,155}$Sm nuclei around 3 MeV in excitation energy and is attributed to the M1 Scissors Resonance (SR). Their integrated strengths were found to be in the range 1.3 - 2.1 and 4.4 - 6.4 $\mu^{2}_{N}$ for $^{153}$Sm and $^{155}$Sm, respectively. The strength of the SR for $^{155}$Sm is comparable to those for deformed even-even Sm isotopes from nuclear resonance fluorescence measurements, while that of $^{153}$Sm is lower than expected